New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	872790 =	28 (1.020)	5 (0.200)	5/196	1.7	17.3%	coding (366/423 nt)	ihfB	integration host factor beta subunit
	?	AM260479	872809 =	22 (0.880)					coding (385/423 nt)	ihfB	integration host factor beta subunit
Rejected: Frequency below/above cutoff threshold.

CAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATTGCTGCCGGCCTTGCGTGCGCACC  >  AM260479/872704‑872936                                                                                                          |                                                                                                                                cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                                      <  1:1054944/133‑1 (MQ=255) cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                                      <  1:579127/133‑1 (MQ=255) cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                                      >  2:579127/1‑133 (MQ=255) cagccGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTcc                                                                                                      >  2:1054944/1‑133 (MQ=255)       gcgcCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCTGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTc                                                                                               >  1:530662/1‑134 (MQ=255)            tCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGAt                                                                                          >  2:871322/1‑134 (MQ=255)                ggCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGcc                                                                                      >  2:620058/1‑134 (MQ=255)                  accacacaagcagcaggcaaaacccggcaatcaggaagagcggacaaaattcagcccaccctcgtgcaatcccgccgctgcggcgggaTTGCACGAGGGTTGGCTGAATTTTGTCCGCTCTTCCTGATTGCCgg                                                                                    <  2:936386‑M2/46‑1 (MQ=255)                        tGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTg                                                                              <  2:570055/134‑1 (MQ=255)                          agcagcaggcaaaacccggcaatcaggaagagcggacaaaattcagcccaccctcgtgcaatcccgccgctgcggcgggaTTGCACGAGGGTGGGCTGAATTTTGTCCGctct                                                                                                 >  2:704498‑M2/81‑113 (MQ=255)                          agcagcaggcaaaacccggcaatcaggaagagcggacaaaattcagcccaccctcgtgcaatcccgccgctgcggcgggaTTGCACGAGGGTGGGCTGAATTTTGTCCGctct                                                                                                 <  1:704498‑M2/33‑1 (MQ=255)                                 tCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCTGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTgctgct                                                                     <  2:530662/134‑1 (MQ=255)                                         ccggcaatcaggaagagcggacaaaattcagccaaccctcgtgcaaTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGt                                                             <  2:936386‑M1/88‑1 (MQ=255)                                              aCGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaa                                                        <  1:620058/134‑1 (MQ=255)                                                ggCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaaa                                                      <  1:871322/134‑1 (MQ=255)                                                      ggCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGcc                                                >  2:783260/1‑134 (MQ=255)                                                      agagcggacaaaattcagcccaccctcgtgcaaTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTgctgct                                                                     >  2:704498‑M1/34‑113 (MQ=255)                                                      agagcggacaaaattcagcccaccctcgtgcaaTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTgctgct                                                                     <  1:704498‑M1/80‑1 (MQ=255)                                                              cGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAgg                                        >  2:100270/1‑134 (MQ=255)                                                                ggCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGt                                                                                                                      >  1:736906/1‑54 (MQ=255)                                                                ggCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGt                                                                                                                      <  2:736906/54‑1 (MQ=255)                                                                ggCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAgggg                                      >  2:738303/1‑134 (MQ=255)                                                                     ggTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCtt                                 >  2:148375/1‑134 (MQ=255)                                                                         ggCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCttttt                              <  1:953992/133‑1 (MQ=255)                                                                             aGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTAtt                         <  2:972238/134‑1 (MQ=255)                                                                                  aGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATtgctgc                    <  1:783260/134‑1 (MQ=255)                                                                                   gcgcTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaa                                                       >  1:81028/1‑98 (MQ=255)                                                                                   gcgcTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaa                                                       <  2:81028/98‑1 (MQ=255)                                                                                        cccgccgctgcggcgggaTTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaaaa                                                     >  2:505345‑M2/19‑95 (MQ=255)                                                                                        cccgccgctgcggcgggaTTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGaaaaaa                                                     <  1:505345‑M2/77‑1 (MQ=255)                                                                                           GCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTTAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATTGCTGCCGGCCTtgc           <  1:205849‑M1/134‑1 (MQ=255)                                                                                                     cgggaTTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATTGCTGCCGGCCTTGCGTGCGCAcc  >  1:504876‑M2/6‑133 (MQ=255)                                                                                                          |                                                                                                                                CAGCCGGCGCCTCCGGGCGCCAATGGCAACGGTCATGCCGCCGGCACGGCCCAGGCCGTGCCGGGCAAGGTAGGCCAGGGCAGCGCTCCCGCCGCAGCGGCGGGATTGCACGAGGGTGGGCTGAATTTTGTCCGCTCTTCCTGATTGCCGGGTTTTGCCTGCTGCTTGTGTGGTTGAAAAAAGCGCCCCCTTAGGGGCGCTTTTTTTATTGCTGCCGGCCTTGCGTGCGCACC  >  AM260479/872704‑872936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.