| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | AM260479 | 3,399,144 | Δ1,321 bp | [leuS]–h16_A3140 | [leuS], h16_A3140 | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | AM260479 | 3399144–3400451 | 3400464 | 14–1321 | 24 [0] | [0] 24 | [leuS]–h16_A3140 | [leuS],h16_A3140 |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | AM260479 | = 3399143 | NA (NA) | 23 (0.910) | 20/198 | 0.3 | NA | noncoding (2/1313 nt) | ISAE1 | insertion sequence ISAE1 |
| ? | AM260479 | 3400465 = | NA (NA) | intergenic (+32/+53) | h16_A3140/dapB | Transposase ISAE1/dihydrodipicolinate reductase | |||||
GGTCGGGACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAGCCTCTTTTGAATTTCAAGTGGGTGGTATCGTGGCGGGTTTCAGGTAAAGAGCAACAAGAGCGATGCATAGCAAACTGTTTGAAGCAGCCCTTGGCATAAGCGATCCGTGGTTCGTCCGGGAAGTCGACT > AM260479/3399015‑3399273 | ggTCGGGACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgat > 1:1158586‑M1/1‑129 (MQ=255) gACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataacc > 2:128320‑M1/1‑123 (MQ=255) cagcagTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgt < 1:296190‑M1/134‑14 (MQ=255) agcagTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtg < 2:1158586‑M1/134‑15 (MQ=255) aGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcag > 2:77303‑M1/1‑113 (MQ=255) cAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggc > 2:388939‑M1/1‑109 (MQ=255) gcgcGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggc > 1:352174‑M1/1‑101 (MQ=255) gCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgcccc < 2:42700‑M1/134‑46 (MQ=255) gCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgcccc < 2:352174‑M1/134‑46 (MQ=255) cATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggca > 2:162654‑M1/1‑80 (MQ=255) cATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggca < 1:162654‑M1/100‑21 (MQ=255) aaTCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggc > 2:235654‑M1/1‑70 (MQ=255) aTCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcc < 1:77303‑M1/134‑66 (MQ=255) gTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggccca < 1:388939‑M1/133‑68 (MQ=255) gAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacg < 1:235654‑M1/134‑73 (MQ=255) gAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacg < 2:947773‑M1/134‑73 (MQ=255) gcgcGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcg < 1:608981‑M1/120‑85 (MQ=255) gcgcGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcg > 2:608981‑M1/1‑36 (MQ=255) cggcgCCCGCCGGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggctt > 2:427718‑M1/1‑33 (MQ=255) gccgccggGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggcttgtcgg > 2:730918‑M1/2‑28 (MQ=255) ccgccgGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgcctta < 2:1000579‑M1/83‑57 (MQ=255) ccgccgGGCGTGCAATGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgcctga > 1:1000579‑M1/1‑27 (MQ=255) aTGCGAAAGAAGAcgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggcttgtcggcgaggaaacgggcgg < 1:730918‑M1/134‑122 (MQ=255) agacgattataaccgtggcggcaggccagtttcggccctgcgcgccccgcggcgccttacttcaggcccagcacgtcctgcatgtcgaacaggccgttgggcttgtcggcgaggaaacgggcggcgcgcacgg > 1:383711‑M1/1‑3 (MQ=255) | GGTCGGGACAGCAGTCAGGACAACGTTTGCGCGGCAACTGGCCTGCCGGCATCCGGGGGAATCGTTGGAATGAGAGCAGCGGGCGCGCCGCTTGCGCGGCGCCCGCCGGGCGTGCAATGCGAAAGAAGAGCCTCTTTTGAATTTCAAGTGGGTGGTATCGTGGCGGGTTTCAGGTAAAGAGCAACAAGAGCGATGCATAGCAAACTGTTTGAAGCAGCCCTTGGCATAAGCGATCCGTGGTTCGTCCGGGAAGTCGACT > AM260479/3399015‑3399273 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |