New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AM260479	= 330490	16 (0.620)	4 (0.170)	4/196	1.8	26.1%	coding (1408/2553 nt)	nagF	Protein‑N(pi)‑phosphohistidine‑sugar phosphotransferase II ABC
	?	AM260479	= 330641	8 (0.340)					coding (1559/2553 nt)	nagF	Protein‑N(pi)‑phosphohistidine‑sugar phosphotransferase II ABC

GCCGACGGCGATGTCATCGTGCTGGCCGCCGGGGAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAGCGCGCTGGAGCGCCTGCAGGACGGCACGCCGGTCATCGCCGACGGCGACAGCGCCCGCCTGCACG  >  AM260479/330385‑330619                                                                                                          |                                                                                                                                  gccgACGGCGATGTCATCGTGCTGGCCGCCGGGGAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATcgcgcg                                                                                                       <  2:73400/134‑1 (MQ=255)  ccgACGGCGATGTCATCGTGCTGGCCGCCGGGGAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGTGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGt                                                                                                      >  2:579048/1‑134 (MQ=255)                          ccgccgGGGAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCtggtg                                                                              >  2:540453/1‑134 (MQ=255)                            gccgGGGAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCt                                                                                                                             >  2:138544/1‑85 (MQ=255)                            gccgGGGAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCt                                                                                                                             <  1:138544/85‑1 (MQ=255)                               ggggAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGctct                                                                         >  2:163690/1‑134 (MQ=255)                                         cGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAgcgcg                                                               <  1:579048/134‑1 (MQ=255)                                                                gACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATcgcgcg                                                                                                       <  1:305893/71‑1 (MQ=255)                                                                gACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATcgcgcg                                                                                                       >  2:1011469/1‑71 (MQ=255)                                                                gACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATcgcgcg                                                                                                       <  1:1011469/71‑1 (MQ=255)                                                                gACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATcgcgcg                                                                                                       >  2:305893/1‑71 (MQ=255)                                                                          gggTTGCCGCACTGGTGATGGCGCGCGGCGGCCagcaaatgcgcatcggg                                                                                                                  >  1:581217‑M1/1‑33 (MQ=255)                                                                          gggTTGCCGCACTGGTGATGGCGCGCGGCGGCCagcaaatgcgcatcggg                                                                                                                  <  2:581217‑M1/50‑18 (MQ=255)                                                                               gCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAGCGCGCTGGAGCGCCTGCAGGACGGCACGCCGGTCATCGCCGa                         >  2:443295/1‑134 (MQ=255)                                                                                    aCTGGTGATGGCGCGCGGCGGCCagcaaatgcgcatcggggcaggcgtgcaggcgggcgctgtcgccgtcggcgatgaccggcgtgccgtcctgcaggcgc                                                     <  1:204522‑M1/101‑79 (MQ=255)                                                                                    aCTGGTGATGGCGCGCGGCGGCCagcaaatgcgcatcggggcaggcgtgcaggcgggcgctgtcgccgtcggcgatgaccggcgtgccgtcctgcaggcgc                                                     >  2:204522‑M1/1‑23 (MQ=255)                                                                                              gcgcgcGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAGCGCGCTGGAGCGCCTGCAGGACGGCACGCCGGTCATCGCCGACGGCGACAGcgcccg          <  1:163690/134‑1 (MQ=255)                                                                                                      cggcCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAGCGCGCTGGAGCGCCTGCAGGACGGCACGCCGGTCATCGCCGACGGCGACAGCGCCCGCCTGCACg  >  2:410257/1‑134 (MQ=255)                                                                                                       ggcCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAGCGCGCTGGAGCGCCTGCAGGACGGCACGCCGGTCATCGCCGACGGCGACAGcgcccg          <  2:1022646/125‑1 (MQ=255)                                                                                                       ggcCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAGCGCGCTGGAGCGCCTGCAGGACGGCACGCCGGTCATCGCCGACGGCGACAGcgcccg          >  1:1022646/1‑125 (MQ=255)                                                                                                          |                                                                                                                                  GCCGACGGCGATGTCATCGTGCTGGCCGCCGGGGAATTCACGCCGTCCGACCTGACCGGACTGGACCGCACCCGGGTTGCCGCACTGGTGATGGCGCGCGGCGGCCCGACCTCGCACGCGGCCATCATCGCGCGTCAGCTCGGCATCCCTTGCCTGGTGGCTCTGGGCAGCGCGCTGGAGCGCCTGCAGGACGGCACGCCGGTCATCGCCGACGGCGACAGCGCCCGCCTGCACG  >  AM260479/330385‑330619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.