New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	5432919 =	31 (0.620)	3 (0.060)	3/318	NT	10.7%	coding (2345/2529 nt)	hrpB	ATP‑dependent helicase HrpB
	?	AE015451	5433100 =	21 (0.450)					coding (2164/2529 nt)	hrpB	ATP‑dependent helicase HrpB

ATGATTTTTTCGGCCTGCCGCACTCGGTGGTGTGCATCAGCTCCCTCTCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGTTGCGGCAGCGGCCAGGGCAGCAGGTTGCGCAGCATCGACGGCAAGTCCAGCGCGGCAAAGTGGCTGAG  >  AE015451/5432699‑5433142                                                                                                                                                                                                                             |                                                                                                                                                                                                                                aTGATTTTTTCGGCCTGCCGCACTCGGTGGTGTGCATCAGCTCCCTCTCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCAt                                                                                                                                                                                                                  >  1:406423/1‑236 (MQ=255)                     cACTCGGTGGTGTGCATCAGCTCCCTCTCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCg                                                                                                                                                                                              <  1:896863/236‑1 (MQ=255)                              gtgtGCATCAGCTCCCTCTCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGcc                                                                                                                                                                                      <  1:346321/235‑1 (MQ=255)                               tgtgCATCAGCTCCCTCTCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGcc                                                                                                                                                                                      <  2:329358/234‑1 (MQ=255)                                   cgtCAGCTCCCTCGCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACctg                                                                                                                                                                                                                             <  2:181512/189‑1 (MQ=255)                                       ctggcaggcgcgtgtggcgctgctgcgtcagctcgacctgcagaaggatggccacagcgaatggcctgacctgggggacgaagccctgctggccagcctggaagactggttacagccttacctgagcaaagtatcgcgcctcagccactttgccgcgctggacttgccgtcgatgctgcgcaACctgctg                                                                                                                                                                                                                          >  2:192731‑M1/183‑190 (MQ=255)                                         cTCCCTCTCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTg                                                                                                                                                                          >  1:82538/1‑236 (MQ=255)                                              cctcGGCTTGGCCCTGGCGGTATCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAAc                                                                                                                                                                                  <  2:516468/222‑1 (MQ=255)                                                 ctgCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACctgc                                                                                                                                                                                                                            <  1:58258/176‑1 (MQ=255)                                                 cGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTc                                                                                                                                                                             <  2:767294/225‑1 (MQ=255)                                                 cGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGGGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGa                                                                                                                                                                                                            >  1:19707/1‑194 (MQ=255)                                                      ggcgctgctgcgtcagctcgacctgcagaaggatggccacagcgaatggcctgacctgggggacgaagccctgctggccagcctggaagactggttacagccttacctgagcaaagtatcgcgcctcagccactttgccgcgctggacttgccgtcgatgctgcgcaACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCAc                                                                                                                                                                 >  1:11862‑M1/168‑232 (MQ=255)                                                                ggTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGCCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCgg                                                                                                                                                                                                                      <  2:914983/169‑1 (MQ=255)                                                                 gTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATcctgt                                                                                                                                                   >  1:59869/1‑231 (MQ=255)                                                                         ggCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGc                                                                                                                                          <  1:834091/236‑1 (MQ=255)                                                                                  agGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGTTGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATc                                                                                                                                                       <  1:510512/214‑1 (MQ=255)                                                                                                  tagtGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCCCCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTc                                                                                                                                            <  1:150238/209‑1 (MQ=255)                                                                                                                     ccttCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGAc                                                                                                                                                                     <  2:59869/163‑1 (MQ=255)                                                                                                                      tttCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGaa                                                                                                                                                                                   >  2:671537/1‑150 (MQ=255)                                                                                                                      tttCAGGTCTTTCGTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCACGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAg                                                                                                                                                                       <  1:273328/162‑1 (MQ=255)                                                                                                                               ttgcttcACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCt                                                                                    <  1:297951/233‑1 (MQ=255)                                                                                                                                       ccTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTcc                                                                            <  2:82538/236‑1 (MQ=255)                                                                                                                                        cTCGGCATAGGTCGGGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGacg                                                                                                            <  1:313196/203‑1 (MQ=255)                                                                                                                                           ggCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGa                                                                                                                    >  2:782245/1‑192 (MQ=255)                                                                                                                                              aTAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGt                                                                      >  1:348205/1‑235 (MQ=255)                                                                                                                                                      tGCGCCAGAAACTGTCCAGGTCCTGGGTCCCCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGCCCATTGGCGATGCGCGGGGTGTCGGCa                                                                                                                                                                                           <  2:50313/110‑1 (MQ=255)                                                                                                                                                        cgcCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATcccg                                                                                                                                >  1:300749/1‑165 (MQ=255)                                                                                                                                                         gcCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCggg                                                                                                                                                 >  1:266715/1‑149 (MQ=255)                                                                                                                                                                            cTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGTTGCGGCAGCGGCCAGGGCAGCAGGTTGCgca                                       >  1:558921/1‑236 (MQ=255)                                                                                                                                                                                gcctcagccactttgccgcgctggacttgccgtcgatgctgcgcaACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGtt                                                                                                                       <  2:397745‑M1/107‑1 (MQ=255)                                                                                                                                                                                gcctcagccactttgccgcgctggacttgccgtcgatgctgcgcaACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGtt                                                                                                                       >  1:397748‑M1/46‑152 (MQ=255)                                                                                                                                                                                       gCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACCGCCAAATgcgc                                                                                            >  1:341368/1‑172 (MQ=255)                                                                                                                                                                                            ggCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGTTGCGGCAGCGGCCAGGGCAGCAGGTTg                                           <  2:682300/216‑1 (MQ=255)                                                                                                                                                                                                                 gggTGCAGCTTGACCTGTTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGTTGCGGCAGCGGCCAGGGCAGCAGGTTGCGCAGCATCGACGGCAAGTCCAGCGCGGCAAAGTGGCTGAg  <  2:868535/235‑1 (MQ=255)                                                                                                                                                                                                                   gTGCAGCTTGACCTGTTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCgggg                                                                                                                                                <  2:731749/92‑1 (MQ=255)                                                                                                                                                                                                                      cAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGGTTCCCTGTAATCCAgggg                                                                                                                             >  1:224343/1‑106 (MQ=255)                                                                                                                                                                                                                        gCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGTTGCGGCAGCGGCCAGGGCAGCAGGTTGCGCAGCATCGACGGCAAGTc                       <  2:674844/208‑1 (MQ=255)                                                                                                                                                                                                                         cTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTaa                                                                                                                                     <  1:287373/97‑1 (MQ=255)                                                                                                                                                                                                                         cTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGGTTCGCTGTaa                                                                                                                                     >  2:287370/1‑97 (MQ=255)                                                                                                                                                                                                                             ACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAGCAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGTTGCGGCAGCGGCCAGGGCAGCAGGTTGCgca                                       >  2:589063‑M1/1‑187 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                                                ATGATTTTTTCGGCCTGCCGCACTCGGTGGTGTGCATCAGCTCCCTCTCGGCTTGGCCCTGGCGGTAGCCTCGGCCACCAGAGGGTCGTCCGGCCAGTAGTGCTTCGGATATCGCCCTTTCAGGTCTTTCTTCACCTCGGCATAGGTCGTGCGCCAGAAACTGGCCAGGTCCTGGGTCACCTGCACCGGCCGACGCGCCGGTGACAGCAGGTGCAGCTTGACCTGCTGCCGGCCATTGGCGATGCGCGGGGTGTCGGCAAGGCCGAACAGTTCTTGCAGACGCACGGCAAGAATCGGCGGGGTTTCGCTGTAATCCAGGCGGATGTTCGAGCCGGACGGCACGGCCAAATGCGCCGGGGCCTGTTCTTCCAGGCGTTGCGGCAGCGGCCAGGGCAGCAGGTTGCGCAGCATCGACGGCAAGTCCAGCGCGGCAAAGTGGCTGAG  >  AE015451/5432699‑5433142

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.