New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 6041484	96 (1.120)	4 (0.050)	4/338	NT	4.0%	coding (440/723 nt)	rph	Ribonuclease PH
	?	AE015451	= 6041615	103 (1.260)					coding (309/723 nt)	rph	Ribonuclease PH
Rejected: Frequency below/above cutoff threshold.

ATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCTGGAACGGTGCGCCTTCGGCAGTACCCTGCACTTCGATGAAACCACCAGCGCTGGTCATGACCACATTCAGGTCGGTTTCGGCTGCGGAGTCTTCCAGGTAGTCCAGGTCGAGCACCGCTTCGCCCTGATACATGCCTACCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCGCCGCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/6041244‑6041484 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgcgctGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTGTGCGACGCTTTGGCAGTGATCAAGAAGCGCGGCGGCCTCAAGGGCGGCAACCCGCTCAAGCACATGATCGCTGCCGTGTCGGTAGGCATGTATCAGGGCGAAGCGGTGCTCGACCTGGAC  <  AE015451/6041615‑6041390                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     ATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCTGGAACGGTGCGCCTTCGGCAGTACCCTGCACTTCGATGAAACCACCAGCGCTGGTCATGACCACATTCAGGTCGGTTTCGGCTGCGGAGTCTTCCAGGTAGTCCAGGTCGAGCACCGCTTCGCCCTGATACATGCCTACCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCGCC                                                                                                                                                                                                                                         >  1:140716/1‑236                                                         GCCTTCGGCAGTACCCTGCACTTCGATGAAACCACCAGCGCTGGTCATGACCACATTCAGGTCGGTTTCGGCTGCGGAGTCTTCCAGGTAGTCCAGGTCGAGCACCGCTTCGCCCTGATACATGCCTACCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCGCCGCG                                                                                                                                                                                                                                      >  2:355840/1‑183                                                                            ACTTCGATGAAACCACCAGCGCTGGTCATGACCACATTCAGGTCGGTTTCGGCTGCGGAGTCTTCCAGGTAGTCCAGGTCGAGCACCGCTTCGCCCTGATACATGCCTACCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGCGGCC                                                                                                                                                                                                                                            >  2:178107/1‑158                                                                                                                   AGGTCGGTTTCGGCTGCGGAGTCTTCCAGGTAGTCCAGGTCGAGCACCGCTTCGCCCTGATACATGCCTACCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCGC                                                                                                                                                                                                                                          <  2:734753/121‑1                                                                                                                                                                 CCGCTTCGCCCTGATACATGCCTGCCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCG                                                                                                                                                                                                                                           <  2:1351056/74‑1                                                                                                                                                                            TGATACATGCCTACCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCGCCGCGCTGGACATGAGCAAGCTCGGTGA                                                                                                                                                                                                               >  2:496956/1‑91                                                                                                                                                                                           GACACGGCAGCGAGCATGTGCTTGATCGGGTTGCCGCCCTTGAGGCCGCCGCGCTGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTGTGCGACGCTTTGGCAGGGATCAAGAAGCGCGGCGGCCT                                                                                    >  1:787514/1‑199                                                                                                                                                                                                      GATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCGCCGCGCTGGACATTAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGG                                                                                                                                  >  2:541638/1‑142                                                                                                                                                                                                          ATGTGCTTGAGCGGGTTGCCGCCCTTGAGTCCGCCGCGCTGGACATGAGCAAGCTCGGTGACATCACCCGGTACGTCGATTGCGACGTGATCCAGGCCGATGGC                                                                                                                                                                    >  1:554562/1‑104                                                                                                                                                                                                                                          GCCGCGCTGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTGTGCGACGCTTTGGCAGTGATCAAGAAGCGCGGCGGCCTCAAGGGCGGCAACCCGCTCAAGCACATGATCGCTGCCGTGTCGGTAGGCATGTATCAGGGCGAAGCGGTGCTCGACCTGGAC  <  1:65464/234‑1                                                                                                                                                                                                                                          GCCGCGCTGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTGTGCGACGCTTTGGCAGTGATCAAGAAGCGCGGCGGCCTCAAGGGCGGCAACCCGCTCAAGCACATGATCGCTGCCGTGTCGGTAGGCATGTATCAGGGCGAAGCGGTGCTC           <  2:1441409/225‑1                                                                                                                                                                                                                                            CGCGCTGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGAT                                                                                                                                                                       <  2:742834/67‑1                                                                                                                                                                                                                                             GCGCTGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTGTGCGACGCTTTGGCAGTGATCAAGAAGCGCGGCGGCCTCAAGGGCGGCAACCCGCTCAAGCACATGATCGCTGCCGTGTCGGTAGGCATGTATCAGGGCGAAGCGG                <  1:1236930/217‑1                                                                                                                                                                                                                                              CGCTGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCAATGGTTGCCTTGTGCGACGCTTTGGCAGTGATCAAGAAGCGCGGCGGCCTCAAGGGCGGCAACCCGCTCAAGCACATGATCGCTGCCGTGTCGGTA                                      >  1:1127303/1‑194                                                                                                                                                                                                                                               GCTGGCCATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTG                                                                                                                          <  2:660942/109‑1                                                                                                                                                                                                                                               GCTGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTGTGCGACGCTTTGGCAGTGATCAAGAAGCGCGGCGGCCTCAAGGGCGGCAACCCGCTCAAGCACA                                                          >  2:305199/1‑173                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     ATGCCCTTCTGCGCCAAAGCGAGCATGGCGTTGAAATCTTCCGGCTGGAACGGTGCGCCTTCGGCAGTACCCTGCACTTCGATGAAACCACCAGCGCTGGTCATGACCACATTCAGGTCGGTTTCGGCTGCGGAGTCTTCCAGGTAGTCCAGGTCGAGCACCGCTTCGCCCTGATACATGCCTACCGACACGGCAGCGATCATGTGCTTGAGCGGGTTGCCGCCCTTGAGGCCGCCGCGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  AE015451/6041244‑6041484 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgccgcgctGGACATGAGCAAGCTCGGTGACATCACCCTGTACGTCGATTGCGACGTGATCCAGGCCGATGGCGGTACCCGTACCGCGTCCATCACCGGCGCCATGGTTGCCTTGTGCGACGCTTTGGCAGTGATCAAGAAGCGCGGCGGCCTCAAGGGCGGCAACCCGCTCAAGCACATGATCGCTGCCGTGTCGGTAGGCATGTATCAGGGCGAAGCGGTGCTCGACCTGGAC  <  AE015451/6041615‑6041390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.