New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1161986	34 (0.780)	3 (0.070)	3/324	NT	8.3%	coding (949/1155 nt)	gtsD	mannose/glucose ABC transporter ‑ ATP binding subunit
	?	AE015451	= 1162087	34 (0.820)					coding (1050/1155 nt)	gtsD	mannose/glucose ABC transporter ‑ ATP binding subunit

CCAGGCAGGATGGCCGCTTGCTGGCGCTGCTCGACAGCGGCCAGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGA  >  AE015451/1161783‑1162214                                                                                                                                                                                                            |                                                                                                                                                                                                                                     ccAGGCAGGATGGCCGCTTGCTGGCGCTGCTCGACAGCGGCCAGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGa                                                                                                                                                                                                        <  2:442036/234‑1 (MQ=255)   aggcaggATGGCCGCTTGCTGGCGCTGCTCGACAGCGGCCAGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCaa                                                                                                                                                                                                    <  1:526171/236‑1 (MQ=255)                            tgctCGACAGCGGCCAGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTGGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGGGCAGATCGCCCTGGGCGCTGCTGCCGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTc                                                                                                                                                                                                               <  1:364135/200‑1 (MQ=255)                                      cGGCCAGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCa                                                                                                                                                                                                                    <  2:22225/185‑1 (MQ=255)                                           aGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACctgctg                                                                                                                                                                                                                              >  2:544572/1‑170 (MQ=255)                                           aGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCCGCTGgtgt                                                                                                                                                                                                                          >  2:186930/1‑174 (MQ=255)                                                               ttGGGGGAGGCTGCTGCCGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCt                                                                                                                                                                                     <  2:186787/191‑1 (MQ=255)                                                                         ctgctgACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTg                                                                                                                                                                                    >  2:393431/1‑182 (MQ=255)                                                                          tgctgACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCgggtgggg                                                                                                                                                         >  1:165835/1‑207 (MQ=255)                                                                                        aGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGtt                                                                                                                                                                                                                         <  1:703477/130‑1 (MQ=255)                                                                                                  gaTCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAAc                                                                                                                                             <  2:666279/196‑1 (MQ=255)                                                                                                  gaTCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAAc                                                                                                                                             >  1:666281/1‑196 (MQ=255)                                                                                                         gtCGGCATCCTCCCTGAGCAGAGCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCGATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCAc                                                                                                                                                                                                                   <  2:200670/118‑1 (MQ=255)                                                                                                         cTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACccggcccgg                                                                                                                        >  2:755733‑M2/1‑201 (MQ=255)                                                                                                                tCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCa                                                                                                                                                                                                                    <  2:739343/111‑1 (MQ=255)                                                                                                                          gCAGATCGCCCTGCGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACcc                                                                                                                               >  2:465668‑M2/1‑184 (MQ=255)                                                                                                                           cAGATCGCCCTGGGCGCTGCGGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGgcgc                                                                                                                                                                                <  2:6705/136‑1 (MQ=255)                                                                                                                            aGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGCTCGTCACGCTCAACCAGACCAAGGAGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGAGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTgctgct                                                                                                                >  1:188208/1‑199 (MQ=255)                                                                                                                                     tGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCATCCAGAcc                                                                                                                                                                                                      <  2:174460/104‑1 (MQ=255)                                                                                                                                      gggCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTc                                                                                                                                    <  2:418578/169‑1 (MQ=255)                                                                                                                                      gggCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTg                                                                    >  2:697803/1‑233 (MQ=255)                                                                                                                                      gggCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGCGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGGGCTGCTGTTCgccgcccc                                                                                                    >  1:219070/1‑199 (MQ=255)                                                                                                                                               tgACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGgtcgaattgcaggttgagggtgtcacccacc                                                                                                                                                                                                       <  1:378364‑M1/93‑32 (MQ=255)                                                                                                                                               tgACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGgtcgaattgcaggttgagggtgtcacccacc                                                                                                                                                                                                       >  2:378362‑M1/1‑62 (MQ=255)                                                                                                                                               tgACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGt                                                                        <  1:544574/220‑1 (MQ=255)                                                                                                                                                       aTGGTTGGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCgggtgg                                                                                                                                                           <  2:21096/129‑1 (MQ=255)                                                                                                                                                           tttGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCCCAACCGCAAAGGACAACGt                                              >  2:290723/1‑234 (MQ=255)                                                                                                                                                                     agccGCGCCGAGGTGCAGGTGACAGAGCCCCCCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGc                                                                                             <  2:85592/175‑1 (MQ=255)                                                                                                                                                                        cgcgCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACccggcccggtgggctctgtcacctgca                                                                                                      >  1:644647‑M2/1‑138 (MQ=255)                                                                                                                                                                            ccGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGt                           <  1:197533/236‑1 (MQ=255)                                                                                                                                                                                  tGCAGGTGACAGAGCCCACCGGGCCGGgtcgaattgcaggttgagggtgtcacccacccggcacgccacatctggcgccaggcggcagcacaccttggtctggttgagcgtgacgaacaccagcaggtccggcccggtgggctctgtcacctgcacctcggcgcg                                                                                            >  1:644647‑M1/1‑27 (MQ=255)                                                                                                                                                                                           cAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTaca              <  1:694826/234‑1 (MQ=255)                                                                                                                                                                                              agCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATc         >  1:58856/1‑236 (MQ=255)                                                                                                                                                                                               gCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCg                            >  1:738098/1‑216 (MQ=255)                                                                                                                                                                                                 ccACCGGGCCGGACCTGCTGGGGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAAtt                                                                                                                                     >  2:44212/1‑109 (MQ=255)                                                                                                                                                                                                  cACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCaaa                                                       <  1:366718/186‑1 (MQ=255)                                                                                                                                                                                                    ccgggccgggtcgaattgcaggttgagggtgtcacccacccggcacgccacatctggcgccaggcggcagcacaccttggtctggttgagcgtgacgaacaccagcaggtccggcccggtgggctctgtcacctgcacctcggcgcggatagccggcaaaccattgccgtcagcagcgcccagggcgatctgctcagggcggatgccgag                             >  2:755733‑M1/1‑9 (MQ=255)                                                                                                                                                                                                    ccGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGt                           >  1:715522/1‑212 (MQ=255)                                                                                                                                                                                                    ccGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGt                           <  2:715520/212‑1 (MQ=255)                                                                                                                                                                                                      gggCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAgg                                                     >  2:802028/1‑184 (MQ=255)                                                                                                                                                                                                      gggCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGa  >  2:367964/1‑235 (MQ=255)                                                                                                                                                                                                           gggtcgaattgcaggttgagggtgtcacccacccggcacgccacatctggcgccaggcggcagcacaccttggtctggttgagcgtgacgaacaccagcaggtccggcccggtgggctctgtcacctgcacctcggcgcggatagccggcaaaccattgccgtcagcagcgcgcagggcgatctgc                                              >  2:465668‑M1/1‑2 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                     CCAGGCAGGATGGCCGCTTGCTGGCGCTGCTCGACAGCGGCCAGGCGCGCTGCGAACTGCCCTTGGGGGAGGCTGCTGACGCGCTTGAGGGGCGCGAGATCATCCTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGA  >  AE015451/1161783‑1162214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.