New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 1161986	34 (0.780)	3 (0.070)	3/324	NT	8.3%	coding (949/1155 nt)	gtsD	mannose/glucose ABC transporter ‑ ATP binding subunit
	?	AE015451	= 1162087	34 (0.820)					coding (1050/1155 nt)	gtsD	mannose/glucose ABC transporter ‑ ATP binding subunit

CTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCACTTGTAGGCAGCAGCGGTACACAGGCTGCCG  >  AE015451/1161887‑1162314                                                                                                                                                                                                         |                                                                                                                                                                                                                                    cTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACccggcccgg                                                                                                                                                                                                                            >  2:755733‑M2/1‑201 (MQ=255)                  gCAGATCGCCCTGCGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACcc                                                                                                                                                                                                                                   >  2:465668‑M2/1‑184 (MQ=255)                    aGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGCTCGTCACGCTCAACCAGACCAAGGAGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGAGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTgctgct                                                                                                                                                                                                                    >  1:188208/1‑199 (MQ=255)                              gggCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTg                                                                                                                                                                        >  2:697803/1‑233 (MQ=255)                              gggCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGCGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGGGCTGCTGTTCgccgcccc                                                                                                                                                                                                        >  1:219070/1‑199 (MQ=255)                                       tgACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGt                                                                                                                                                                            <  1:544574/220‑1 (MQ=255)                                                   tttGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCCCAACCGCAAAGGACAACGt                                                                                                                                                  >  2:290723/1‑234 (MQ=255)                                                             agccGCGCCGAGGTGCAGGTGACAGAGCCCCCCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGc                                                                                                                                                                                                 <  2:85592/175‑1 (MQ=255)                                                                cgcgCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACccggcccggtgggctctgtcacctgca                                                                                                                                                                                                          >  1:644647‑M2/1‑138 (MQ=255)                                                                    ccGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGt                                                                                                                               <  1:197533/236‑1 (MQ=255)                                                                                   cAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTaca                                                                                                                  <  1:694826/234‑1 (MQ=255)                                                                                      agCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATc                                                                                                             >  1:58856/1‑236 (MQ=255)                                                                                       gCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCg                                                                                                                                >  1:738098/1‑216 (MQ=255)                                                                                          cACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCaaa                                                                                                                                                           <  1:366718/186‑1 (MQ=255)                                                                                            ccGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGt                                                                                                                               >  1:715522/1‑212 (MQ=255)                                                                                            ccGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGt                                                                                                                               <  2:715520/212‑1 (MQ=255)                                                                                              gggCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAgg                                                                                                                                                         >  2:802028/1‑184 (MQ=255)                                                                                              gggCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGa                                                                                                      >  2:367964/1‑235 (MQ=255)                                                                                                        tgctgGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACg                                                                                           <  1:367966/236‑1 (MQ=255)                                                                                                        tgctgGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACg                                                                                           <  1:697805/236‑1 (MQ=255)                                                                                                            ggggtTCGTCACGCTCAACCAGCCCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACg                                                                                           <  2:339852/229‑1 (MQ=255)                                                                                                                  cGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTgg                                                                                                                                                >  2:299019/1‑173 (MQ=255)                                                                                                                       cGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAg                                                                                      >  2:731418/1‑226 (MQ=255)                                                                                                                          tCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGGGACACCCTCCACCTGCAATTCGACCCGGCCCGGGTGCTGCTGGTCGACGCCGCCAATGGCGGGCGccc                                                                                                                                                                                         >  2:107336/1‑123 (MQ=255)                                                                                                                          tCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGAGGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCCACCCGGCCCGGCTGCTGCTGTTCGGGGCCGCCACTAGCg                                                                                                                                                                                                >  2:291706/1‑117 (MQ=255)                                                                                                                            acccAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAgcgc                                                                        <  1:166154/233‑1 (MQ=255)                                                                                                                                         tgtgCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGa                                                           >  1:476584/1‑235 (MQ=255)                                                                                                                                               gccgccTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAAcc                                                                                                                                                                >  2:127583/1‑128 (MQ=255)                                                                                                                                               gccgccTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAAcc                                                                                                                                                                <  1:127585/128‑1 (MQ=255)                                                                                                                                                           cAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCctt                                        <  1:523262/236‑1 (MQ=255)                                                                                                                                                                        ccGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATgg                                                                                <  2:738096/183‑1 (MQ=255)                                                                                                                                                                          gggtggttGACACCCTCAACCTGCAATTCGCCCCGGCCCGGGTGCTGCTGTTCGCCGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAg                                                                                                                                    <  2:442579/129‑1 (MQ=255)                                                                                                                                                                           ggtgggtgACACCCTCAACCTGCAATTCGACccggcccggtgggctctgtcacctgcacctcggcgcggatagccggcaaaccattgccgtca                                                                                                                                                                       >  2:378362‑M2/1‑31 (MQ=255)                                                                                                                                                                           ggtgggtgACACCCTCAACCTGCAATTCGACccggcccggtgggctctgtcacctgcacctcggcgcggatagccggcaaaccattgccgtca                                                                                                                                                                       <  1:378364‑M2/93‑63 (MQ=255)                                                                                                                                                                           ggtgggtgACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACg                                                                                           <  1:199537/169‑1 (MQ=255)                                                                                                                                                                                                   cttCGACCCGGCCCGGGGGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATg                                                                                 <  2:252647/154‑1 (MQ=255)                                                                                                                                                                                                   aTTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTg                                             <  1:232749/191‑1 (MQ=255)                                                                                                                                                                                                    ttCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCACTTGTAGGCAGCAGCGGTACACAGGCTGCCg  >  2:370361/1‑233 (MQ=255)                                                                                                                                                                                                    ttCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCCCACTTCAAAGGCCGTTGACGCGTCTACAccct                                                                                                              >  2:36184/1‑123 (MQ=255)                                                                                                                                                                                                      cGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGTATCGTTGGCCTTGCTTGCACTTGTAgg                         <  1:423192/208‑1 (MQ=255)                                                                                                                                                                                                       gACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCAc                                <  1:121186/200‑1 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                    CTCGGCATCCGCCCTGAGCAGATCGCCCTGGGCGCTGCTGACGGCAATGGTTTGCCGGCTATCCGCGCCGAGGTGCAGGTGACAGAGCCCACCGGGCCGGACCTGCTGGTGTTCGTCACGCTCAACCAGACCAAGGTGTGCTGCCGCCTGGCGCCAGATGTGGCGTGCCGGGTGGGTGACACCCTCAACCTGCAATTCGACCCGGCCCGGGTGCTGCTGTTCGACGCCGCCAATGGCGAGCGCCTGCACCTGGCCAGTACTGGCGCAACCGCAAAGGACAACGTGGCTCACTTCAAAGGCCGTTGACTCGTCTACACCATCAATAAGAAAAAGAGGACGCAAAGGGATGGAACAGCGCAAACGCATCAAGACACTGGGATCGTTGGCCTTGCTTGCACTTGTAGGCAGCAGCGGTACACAGGCTGCCG  >  AE015451/1161887‑1162314

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.