New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	4089728 =	37 (0.850)	3 (0.070)	3/326	NT	7.7%	coding (795/912 nt)	kdgD	5‑dehydro‑4‑deoxyglucarate dehydratase
	?	AE015451	4089763 =	37 (0.890)					coding (830/912 nt)	kdgD	5‑dehydro‑4‑deoxyglucarate dehydratase

CTGCCGACCGCCGAAGTGTATGCCGCCGCCTACAAGGCCCTGGGCGTACCGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCACTCCGGCTACAATTCGTGAGCCCGCACAAAAATAACTAGTGG  >  AE015451/4089510‑4089936                                                                                                                                                                                                                           |                                                                                                                                                                                                                 agcgcacatgcgttttcttagtcgcttgcatgctggcaatactcccactagttatttttgtgcgggctcacgaattgtagccggagtggggtaggcccactccgggcgcggcgtctggcaggccgcgggcagcgtttactgcgggcccatcttgtccatcagcgcggcgagcatttcgtactcttcggcggtggggtcggtcagcggggtacgcaccgGGCCggcgg                                                                                                                                                                                                          <  1:136265‑M1/9‑2 (MQ=255)     cGACCGCCGAAGTGTATGCCGCCGCCTACAAGGCCCTGGGCGTACCGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCgg                                                                                                                                                                                                             >  1:41445/1‑220 (MQ=255)                 tgtATGCCGCCGCCTACAAGGCCCTGGGCGTACCGGTGTCCTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACATGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCgg                                                                                                                                                                                  <  1:537046/235‑1 (MQ=255)                    aTGCCGCCGCCTACAAGGCCCTGGGCGTACCGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTacgacg                                                                                                                                                                                      <  2:798802/228‑1 (MQ=255)                              cTACAAGGCCCTGGGCGTACCGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTAcc                                                                                                                                                                     <  1:459940/235‑1 (MQ=255)                                           ggCGTACCGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTgaccgacc                                                                                                                                                        <  1:295466/235‑1 (MQ=255)                                            gCGTACCGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCt                                                                                                                                                       >  1:464302/1‑235 (MQ=255)                                                  cGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCAccg                                                                                                                                                  >  2:550631/1‑234 (MQ=255)                                                   ggTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTacg                                                                                                                                                                                         >  2:801151/1‑194 (MQ=255)                                                                              cGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTg                                                                                                                                                               >  1:256694/1‑193 (MQ=255)                                                                                 gCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGAt                                                                                                                   >  2:501563/1‑234 (MQ=255)                                                                                    cgaaaCCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACa                                                                                                              >  1:670704/3‑236 (MQ=255)                                                                                       ccccGCCATGGACTGCTACAACGCCATCGCCCGTGACGCCCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTa                                                                                                                                                                       <  2:120631/174‑1 (MQ=255)                                                                                                ggACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCgg                                                                                                                                                                                  >  1:59498/1‑156 (MQ=255)                                                                                                ggACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCgg                                                                                                                                                                                  <  2:59497/156‑1 (MQ=255)                                                                                                       tACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCcgcg                                                                                                                        >  2:574669/1‑207 (MQ=255)                                                                                                       cgggcgcggcgtctggcaggccgcgggcagcgtttactgcgggcccatcttgtccatcagcgcggcgagcatttcgtactcttcggcggtgaggtcggtcagcgggggacgcaccgGGCCGgcg                                                                                                                                                                                                           <  1:76779‑M1/8‑1 (MQ=255)                                                                                                             gCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCgg                                                                                                                                                                                  <  1:430867/143‑1 (MQ=255)                                                                                                                aTCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCcgc                                                                                  <  1:698968/236‑1 (MQ=255)                                                                                                                    cccGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACg                                                                                          >  2:698966/1‑224 (MQ=255)                                                                                                                            gcccACGCCACCGTGGCCAAGCTGCTCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGc                                                                                                                                <  1:248659/176‑1 (MQ=255)                                                                                                                                  gCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCcgcg                                                                >  1:595350/1‑236 (MQ=255)                                                                                                                                           gCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTg                                                       <  1:699325/236‑1 (MQ=255)                                                                                                                                            ccAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGAc                                                                                                               >  2:727707/1‑179 (MQ=255)                                                                                                                                                      cgacgaTTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTAc                                                <  2:766927/232‑1 (MQ=255)                                                                                                                                                      cgacgaTTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCa                                            <  1:398859/236‑1 (MQ=255)                                                                                                                                                                   cgcggcgagcatttcgtactcttcggcggtgaggtcggtcaggggggtacgcaccgGGCCGGCGCCAGAATTGACGGTTACGCCGACGGCCCGGTGCGTACCCCGCt                                                                                                                                                                <  2:631102‑M1/51‑1 (MQ=255)                                                                                                                                                                         tACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGAcgc                                                                    >  2:369809/1‑193 (MQ=255)                                                                                                                                                                         tACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCACTCCGGCTACAATTCGTg                          >  1:724241/1‑235 (MQ=255)                                                                                                                                                                             tGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGc                                                                                                  >  1:299272/1‑159 (MQ=255)                                                                                                                                                                                aCATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCACTCCGGCTACAATTCGTGAGccc                     <  2:670702/233‑1 (MQ=255)                                                                                                                                                                                    ccgcaaccgcaaGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCACTCCGGCTACAATTCGTGa                         <  1:61186/225‑1 (MQ=255)                                                                                                                                                                                     cgcaaccgcaaGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCg                                                            >  1:93937/1‑189 (MQ=255)                                                                                                                                                                                        aaccgcaaGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTcgcc                                                                                                                           <  2:22514/123‑1 (MQ=255)                                                                                                                                                                                        aaccgcaaGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTcgcc                                                                                                                           >  1:22514/1‑123 (MQ=255)                                                                                                                                                                                        aaccgcaaGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCgg                                                           >  2:312685/1‑187 (MQ=255)                                                                                                                                                                                          cggcggtgaggtcggtcagcggggtacgcaccgGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGAGCGt                                                                                                                                                                        <  2:615171‑M1/43‑1 (MQ=255)                                                                                                                                                                                            gcggtgagggcggtcagcggggtacgcaccgGGCCGGCGCCAGAATTGCCGGTTacgacg                                                                                                                                                                                      <  1:711172‑M1/29‑1 (MQ=255)                                                                                                                                                                                            gcaaGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCACTcc                                        <  2:318777/202‑1 (MQ=255)                                                                                                                                                                                                ggCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGACGCGCCCGGAGTGGGCCTACCCCACTCCGGCTACAATTCGTGAGCCCGCACACAAATAACTAGTgg  >  1:268862/1‑236 (MQ=255)                                                                                                                                                                                                        aCGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCgg                                                                                                                                                                                  >  2:136258/1‑52 (MQ=255)                                                                                                                                                                                                                  cATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGAcgccgc                                                                 <  2:663612/155‑1 (MQ=255)                                                                                                                                                                                                                        cAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCACTCCGGCTACAATTCGTGAGccc                     <  1:172120/193‑1 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                 CTGCCGACCGCCGAAGTGTATGCCGCCGCCTACAAGGCCCTGGGCGTACCGGTGTACTCCTCCGCTGTGTTCAACTTCGTGCCGAAAACCGCCATGGACTTCTACAACGCCATCGCCCGTGACGACCACGCCACCGTGGCCAAGCTGATCGACGATTTCTTCCTGCCCTACCTGGACATCCGCAACCGCAAGGCCGGCTACGCCGTGAGCATCGTCAAGGCCGGCGCCAGAATTGCCGGTTACGACGCCGGCCCGGTGCGTACCCCGCTGACCGACCTCACCGCCGAAGAGTACGAAATGCTCGCCGCGCTGATGGACAAGATGGGCCCGCAGTAAACGCTGCCCGCGGCCTGCCAGACGCCGCGCCCGGAGTGGGCCTACCCCACTCCGGCTACAATTCGTGAGCCCGCACAAAAATAACTAGTGG  >  AE015451/4089510‑4089936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.