New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5333105	30 (0.690)	4 (0.090)	4/330	NT	12.5%	coding (840/903 nt)	gluQ	Glutamyl‑Q tRNA(Asp) synthetase
	?	AE015451	= 5333190	27 (0.640)					intergenic (+22/‑221)	gluQ/PP_5704	Glutamyl‑Q tRNA(Asp) synthetase/conserved protein of unknown function

GCCAGCTGTACCTGCAGGAACTGCTGGGCTTCTCACAGCCGCGTTACCTGCATATTCCGCTGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGG  >  AE015451/5332879‑5333334                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      gcCAGCTGTACCTGCAGGAACTGCTGGGCTTCTCACAGCCGCGTTACCTGCATATTCCGCTGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCccgcgaag                                                                                                                                                                                                                               <  1:552722‑M1/235‑9 (MQ=255)                    aCTGCTGGGCTTCTCACAGCCGCGTTACCTGCATATTCCGCTGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATcg                                                                                                                                                                                                              >  2:779842/1‑233 (MQ=255)                          gggCTTCTCACAGCCGCGTTACCTGCATATTCCGCTGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGCACCGTTCGCCGCCACTGCTGGCGGAGCATACTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATc                                                                                                                                                                                                               <  1:762806/226‑1 (MQ=255)                                                  gCATATTCCGCTGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTgc                                                                                                                                                                             <  1:565245/236‑1 (MQ=255)                                                             tGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGa                                                                                                                                                                                                                 <  1:270130/189‑1 (MQ=255)                                                             tGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGa                                                                                                                                                                                                                 >  2:270128/1‑189 (MQ=255)                                                             tGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGAc                                                                                                                                                                                                    >  2:702613/1‑202 (MQ=255)                                                                tCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAg                                                                                                                                                                                                                    >  2:721678/1‑183 (MQ=255)                                                                            aTGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTcgcg                                                                                                                                                   >  1:457253/1‑236 (MQ=255)                                                                                    aaGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGgcgc                                                                                                                                              >  2:457251‑M2/1‑229 (MQ=255)                                                                                      gCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAg                                                                                                                                                                                                                    <  2:646732/161‑1 (MQ=255)                                                                                          ggCAAGCCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCg                                                                                                                                                              <  2:317037/211‑1 (MQ=255)                                                                                                       gTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCAcg                                                                                                                        <  1:792525/236‑1 (MQ=255)                                                                                                           gccgccACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCg                                                                                                                                                                                                                  <  2:136280/142‑1 (MQ=255)                                                                                                                   tGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCgcgc                                                                                                                                                                                                           <  2:738879/141‑1 (MQ=255)                                                                                                                        gCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCtgt                                                                                                       <  1:721680/236‑1 (MQ=255)                                                                                                                                     ccccccTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATc                                                                                                                                                                                                               <  2:44223/119‑1 (MQ=255)                                                                                                                                      cccccTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGGCCCGGAACTGCTGTTGGCGCCGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTg                                                                                                                                                                         <  2:151355/156‑1 (MQ=255)                                                                                                                                                ctgcGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCtg                                                                                             >  2:406829/1‑222 (MQ=255)                                                                                                                                                     ggCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGCCCACGGTGCCAGAGGCTGATTTgcgcc                                                                                                                                                                          >  2:220540/1‑139 (MQ=255)                                                                                                                                                     ggCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCa                                                                                                               >  2:458161/1‑199 (MQ=255)                                                                                                                                                       cgcTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAgg                                                                                                            <  1:157400/198‑1 (MQ=255)                                                                                                                                                           gCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCtg                                                                                             >  1:94735/1‑211 (MQ=255)                                                                                                                                                           gCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTGGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTAcc                                                                                                                                                               <  2:187881/145‑1 (MQ=255)                                                                                                                                                                 gcTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCgg                                                               <  1:779844/235‑1 (MQ=255)                                                                                                                                                                  cTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGc                                                              <  1:406831/235‑1 (MQ=255)                                                                                                                                                                     gggCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTgg                                                                                                     <  2:773617/193‑1 (MQ=255)                                                                                                                                                                     gggCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAg                                                          >  1:340349/1‑236 (MQ=255)                                                                                                                                                                                   cccGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGT‑‑CGCGCccgcgaagaggccgggaaaggttcagcgcaaatcagcctctggcaccgtggtccgctgcgcgatcgcttccggccgccattgcg                                                                                                                                                   >  1:244597‑M1/1‑47 (MQ=255)                                                                                                                                                                                        aaCTGCTGTTGGCGCCGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACaa                                       <  1:349385/236‑1 (MQ=255)                                                                                                                                                                                                ttGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCccgcgaagaggccggtaaaggttcagcgcaaatcagc                                                                                                                                                                                                  <  2:373583‑M1/73‑38 (MQ=255)                                                                                                                                                                                                ttGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCccgcgaagaggccggtaaaggttcagcgcaaatcagc                                                                                                                                                                                                  >  1:373585‑M1/1‑36 (MQ=255)                                                                                                                                                                                                ttGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGc                                                                  <  1:329923/201‑1 (MQ=255)                                                                                                                                                                                                tgggcgACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGgcgcgc                                                                                                                                            >  2:767916‑M2/3‑121 (MQ=255)                                                                                                                                                                                                    cgccgacGGCAGAGGTGCTGGCAGTCGCGCGCCCGCAATGGCGGCGGGAAGCGATCGCGCAGCGGCCCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCGTCGCGGGCGCGCCCGCTCGCACAGGAACCACGCCGCATTCAAGGCCTGTgg                                                                                                     <  2:85275/156‑1 (MQ=255)                                                                                                                                                                                                                     cTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGa           <  1:639623/235‑1 (MQ=255)                                                                                                                                                                                                                              gcgcgcccgcgcagaggccggt                                                                                                                                                                                                                       >  2:220536‑M1/1‑6 (MQ=255)                                                                                                                                                                                                                              gcgcgcccgcgaagaggccggtaaaggttcagcgcaaatcagcctctggcaccgtggtccgctgcgcgatcgcttccggccgccattgcgcgcgcgcgactgccagcacctctgccggcgtcgccca                                                                                                              >  2:767916‑M1/1‑6 (MQ=255)                                                                                                                                                                                                                                gcgcgcgcAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTa                                                       <  2:94734/180‑1 (MQ=255)                                                                                                                                                                                                                                gcgcgcgcAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTa                             >  2:510177/1‑206 (MQ=255)                                                                                                                                                                                                                                gcgcccgcgaagaggccggtaaaggttcagcgcaaatcagcctctggcaccgtggtccgctgcgcgatcgcttccggccgccattgcgcgcgcgcgactgccagcacctctgccggcgtcgccaacagcagttccgggtctgtctcctgccccagcgcccgcagtgcccgcagcagcaggggggtagcatgctccgcctgcagtggcggcgaacggtacgacttgcccagctt  >  2:457251‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                      GCCAGCTGTACCTGCAGGAACTGCTGGGCTTCTCACAGCCGCGTTACCTGCATATTCCGCTGATCGTGCAGCCGGATGGGCACAAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGCCGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGG  >  AE015451/5332879‑5333334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.