New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5333105	30 (0.690)	4 (0.090)	4/330	NT	12.5%	coding (840/903 nt)	gluQ	Glutamyl‑Q tRNA(Asp) synthetase
	?	AE015451	= 5333190	27 (0.640)					intergenic (+22/‑221)	gluQ/PP_5704	Glutamyl‑Q tRNA(Asp) synthetase/conserved protein of unknown function

AAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCGTAGCGCCCAACCATCGAGGCCAGCATGTAC  >  AE015451/5332962‑5333416                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    aaGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGgcgc                                                                                                                                                                                                                                 >  2:457251‑M2/1‑229 (MQ=255)                    gTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCAcg                                                                                                                                                                                                           <  1:792525/236‑1 (MQ=255)                                     gCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCtgt                                                                                                                                                                                         <  1:721680/236‑1 (MQ=255)                                                             ctgcGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCtg                                                                                                                                                                               >  2:406829/1‑222 (MQ=255)                                                                  ggCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCa                                                                                                                                                                                                 >  2:458161/1‑199 (MQ=255)                                                                    cgcTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAgg                                                                                                                                                                                              <  1:157400/198‑1 (MQ=255)                                                                        gCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCtg                                                                                                                                                                               >  1:94735/1‑211 (MQ=255)                                                                              gcTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCgg                                                                                                                                                 <  1:779844/235‑1 (MQ=255)                                                                               cTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGc                                                                                                                                                <  1:406831/235‑1 (MQ=255)                                                                                  gggCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTgg                                                                                                                                                                                       <  2:773617/193‑1 (MQ=255)                                                                                  gggCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAg                                                                                                                                            >  1:340349/1‑236 (MQ=255)                                                                                                     aaCTGCTGTTGGCGCCGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACaa                                                                                                                         <  1:349385/236‑1 (MQ=255)                                                                                                             ttGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGc                                                                                                                                                    <  1:329923/201‑1 (MQ=255)                                                                                                             tgggcgACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGgcgcgc                                                                                                                                                                                                                               >  2:767916‑M2/3‑121 (MQ=255)                                                                                                                 cgccgacGGCAGAGGTGCTGGCAGTCGCGCGCCCGCAATGGCGGCGGGAAGCGATCGCGCAGCGGCCCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCGTCGCGGGCGCGCCCGCTCGCACAGGAACCACGC‑CGCATTCAAGGCCTGTgg                                                                                                                                                                                       <  2:85275/156‑1 (MQ=255)                                                                                                                                  cTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGa                                                                                             <  1:639623/235‑1 (MQ=255)                                                                                                                                             gcgcgcgcAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTa                                                                                                                                         <  2:94734/180‑1 (MQ=255)                                                                                                                                             gcgcgcgcAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTa                                                                                                               >  2:510177/1‑206 (MQ=255)                                                                                                                                                  cgcAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTCCCGGCCTCTTCGCGGgcgcgactgccagcacctctgccggcgtcgccaacagcagttccggg                                                                                                                                                                                      >  1:244597‑M2/1‑84 (MQ=255)                                                                                                                                                    cAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCa                                                                                                                  <  1:463684/196‑1 (MQ=255)                                                                                                                                                                               cGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATcccc                                                                  <  1:336326/217‑1 (MQ=255)                                                                                                                                                                                    cACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAAtaataa                                           <  1:376298/235‑1 (MQ=255)                                                                                                                                                                                            cAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCaat                                                >  2:546145/1‑222 (MQ=255)                                                                                                                                                                                               agGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCg                                <  1:458163/235‑1 (MQ=255)                                                                                                                                                                                                 gCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGgcgcgcgactgccagcacctctgccggcgtcgccaa                                                                                                                                                                                                 <  2:373583‑M2/73‑37 (MQ=255)                                                                                                                                                                                                 gCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGgcgcgcgactgccagcacctctgccggcgtcgccaa                                                                                                                                                                                                 >  1:373585‑M2/1‑37 (MQ=255)                                                                                                                                                                                                    gATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGGACCACGC‑CGCCTTCa                                                                                                                                                                                                 >  2:228107/1‑69 (MQ=255)                                                                                                                                                                                                            cTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCGTAGCGCCCAAcc                    <  1:702615/234‑1 (MQ=255)                                                                                                                                                                                                                  cTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCGTAGCGCCCAACCATCg                >  1:444629/1‑232 (MQ=255)                                                                                                                                                                                                                      aCCGGCCTCTGCGCGGgcgcgc                                                                                                                                                                                                                               >  2:220536‑M2/1‑16 (MQ=255)                                                                                                                                                                                                                        cGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGTAGCCCACAGGCCTTGAAGG‑CGGCGTGGTTCCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTg                                                                                                 <  2:3116/145‑1 (MQ=17)                                                                                                                                                                                                                        agctgtgggagcgGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCGTAGCGCCCAACCATCGAGGCCAGc        <  1:176635/224‑1 (MQ=255)                                                                                                                                                                                                                            ctctTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAACTTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCGTCGCGCCCAcccct                  >  2:249366/1‑218 (MQ=255)                                                                                                                                                                                                                              ctTCGCGGgcgcgcgactgccagcacctctgccggcgtcgccaacagcagttccgggtctgtctcctgccccagcgcccgcagtgcccgcagcagcaggggggtagcatgctccgcctgcagtggcggcgaacggtacgacttgcccagcttgtgcccatccggctgcacgatcagcggaatatgcaggtaacgcggctgtgagaagcccagcagttcctgcaggtacagctggc  <  1:552722‑M2/235‑228 (MQ=255)                                                                                                                                                                                                                               ttCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCGTAGCGCCCAACCATCg                <  1:665507/219‑1 (MQ=255)                                                                                                                                                                                                                                  gcgcgcgcGCCCGCTCACAGCGACAACACGC‑CCCCCACAAGGCC‑‑TGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTaaaaa                                                                                                                            <  1:344521/106‑1 (MQ=21)                                                                                                                                                                                                                                  gcgGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCaaa                                                                                                       <  2:233740/129‑1 (MQ=255)                                                                                                                                                                                                                                    gggCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCACGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCgg                                                                                                                                                 >  2:141/1‑85 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    AAGCTGGGCAAGTCGTACCGTTCGCCGCCACTGCAGGCGGAGCATGCTACCCCCCTGCTGCTGCGGGCACTGCGGGCGCTGGGGCAGGAGACAGACCCGGAACTGCTGTTGGCGACGCCGGCAGAGGTGCTGGCAGTCGCGCGCGCGCAATGGCGGCCGGAAGCGATCGCGCAGCGGACCACGGTGCCAGAGGCTGATTTGCGCTGAACCTTTACCGGCCTCTTCGCGGGCGCGCCCGCTCCCACAGGAACCACGC‑CGCCTTCAAGGCCTGTGGGCTACCTGTGGGAGCGGGCATGCCCGCGAAAGGGCCGGCACAGGTACCCCCCCTAAAAACAAAAGCCCAGTAAATTCAAACCCTTGGCGAACGCTGTGGATTCCCGCTAGCATCCCCCCTGCCATTTGCGCCAATAATAAGTTCAAGCCCGTAGCGCCCAACCATCGAGGCCAGCATGTAC  >  AE015451/5332962‑5333416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.