New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 424959	34 (0.780)	4 (0.100)	3/324	NT	11.1%	coding (351/1113 nt)	PP_0349	putative Membrane protein
	?	AE015451	= 425012	32 (0.770)					coding (298/1113 nt)	PP_0349	putative Membrane protein

TGCCGAATTCCAGGCGGCGCATGAACGGCGCATAGAGCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAGGGGCGTCGCCTAGCAGGTGTTCGAGTTCGTGGTGGAAGATAAGGGGCAGGCCGGTGACT  >  AE015451/424778‑425226                                                                                                                                                                                                                                           |                                                                                                                                                                                                                       tGCCGAATTCCAGGCGGCGCATGAACGGCGCATAGAGCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTg                                                                                                                                                                                                                       >  1:387129‑M2/1‑235 (MQ=255)                  cgcATGAACGGCGCATAGAGCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAAt                                                                                                                                                                                                      <  1:743537/236‑1 (MQ=255)                        aaCGGCGCATAGAGCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGa                                                                                                                                                                                                 <  1:539818/235‑1 (MQ=255)                                 tAGAGCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTggcggtgg                                                                                                                                                                                                                >  1:8475‑M2/1‑203 (MQ=255)                                    agCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTgg                                                                                                                                                                                     <  1:223616/235‑1 (MQ=255)                                     gCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTggg                                                                                                                                                                                    >  1:452336/1‑235 (MQ=255)                                      cACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTggcggtggccatgccgg                                                                                                                                                                                                       >  1:409014‑M2/1‑198 (MQ=255)                                                               aTGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTggcggcgggg                                                                                                                                                                                                              <  1:204642‑M2/183‑11 (MQ=255)                                                                               tGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGcggcgg                                                                                                                                                                                                          >  2:710759/1‑171 (MQ=255)                                                                               tGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCgggg                                                                                                                                         <  1:568831/236‑1 (MQ=255)                                                                                 aaGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCg                                                                                                                                       <  1:313887/236‑1 (MQ=255)                                                                                         cagcagcTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAg                                                                                                                                                              >  2:332967/1‑205 (MQ=255)                                                                                                 tGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGt                                                                                                                                                             >  2:439302/1‑198 (MQ=255)                                                                                                                          cacaGGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCCCCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAg                                                                                                                                                                    <  1:238418/166‑1 (MQ=255)                                                                                                                                    cgcAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGGGGCGGCGGTAATGGCGACCACGCCGTTggg                                                                                                                                                                                    >  2:725885/1‑140 (MQ=255)                                                                                                                                      cagcaTCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGAc                                                                                                                                                                                                <  1:217734/126‑1 (MQ=255)                                                                                                                                          aTCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCt                                                                                                                                                 <  1:421055/169‑1 (MQ=255)                                                                                                                                          aTCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCt                                                                                                                                                 >  2:421053/1‑169 (MQ=255)                                                                                                                                          aTCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGtgc                                                                                     >  2:288949/1‑229 (MQ=255)                                                                                                                                               catcatGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCAtt                                                                          <  1:744859/235‑1 (MQ=255)                                                                                                                                                ttcatGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTc                                                                        <  2:614728/235‑1 (MQ=255)                                                                                                                                                    tGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCg                                                                                                                                               <  2:587015/161‑1 (MQ=255)                                                                                                                                                        tCCACCAGTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTc                                                                        <  2:614145/228‑1 (MQ=255)                                                                                                                                                           accaTTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTAGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAg                                                             >  1:762997/1‑236 (MQ=255)                                                                                                                                                            ccaTTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAgg                                                            >  1:546731/1‑236 (MQ=255)                                                                                                                                                                  gcggcCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTggcggtggccatgccggccgccaatggtggatt                                                                                                                                                                                       >  2:278586‑M2/1‑74 (MQ=255)                                                                                                                                                                    ggccggcATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCg                                                                                                                             <  1:346571/163‑1 (MQ=255)                                                                                                                                                                       cggcATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAGGGGCGTCGCCTa                                                 >  1:420078/1‑236 (MQ=255)                                                                                                                                                                             ggCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTc                                                                        <  2:408084/207‑1 (MQ=255)                                                                                                                                                                                     ccTGGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTGGGGGTCGGGGCTggcggtggccatgccggccgccaatggtggattcatgatggtgatgctgc                                                                                                                                                                      >  1:566375‑M2/1‑55 (MQ=255)                                                                                                                                                                                     ccTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTc                                                                        <  2:445325/199‑1 (MQ=255)                                                                                                                                                                                            ggTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATg                                                                                                                                                                                                     >  1:102202/1‑67 (MQ=255)                                                                                                                                                                                              tgcgtgcATCGAGCATGAAGGTGTGCGACAGGTTTGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGTCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTc                                                                        <  2:762783/190‑1 (MQ=255)                                                                                                                                                                                                        gggcaTGAAGGTGTGCGACAGGTTGGGGTCGGTGCTggcggtggccatgccggccgccaatggt                                                                                                                                                                                            >  2:659598‑M2/3‑36 (MQ=255)                                                                                                                                                                                                        gAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTtgc                                                                                                           <  2:738193/145‑1 (MQ=255)                                                                                                                                                                                                          gCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTc                                                                        <  1:542414/178‑1 (MQ=255)                                                                                                                                                                                                               aaGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAGGGGCGTCGCCTAGCAGGTGTTCGAGTTCgtgg                             <  1:77071/216‑1 (MQ=255)                                                                                                                                                                                                                        gACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAGGGGCGTCGCCTAGCAGGTGTTCGAGTTCGTGGTGGAAGATAAGGGGCAGGCCGGTGACt  >  2:383390/1‑234 (MQ=255)                                                                                                                                                                                                                             gTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTc                                                                        <  2:176659/159‑1 (MQ=255)                                                                                                                                                                                                                                   gTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGtt                                                                                                                                                                                       <  2:214563/42‑1 (MQ=255)                                                                                                                                                                                                                                          cTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGCTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAGGGGCGTCGCCTAGCAGGTGTTCGAGtac                                 >  1:442078/1‑183 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                       TGCCGAATTCCAGGCGGCGCATGAACGGCGCATAGAGCACGGTGCCGGAGACAATCGCGACAATGAACAATACGCCCATGAAGGCCAGCAGCAGCTTGCCCGGCAGGCCTGCGAACATGTCCACATGCAGGCGCAGCATCACCATCATGAATCCACCATTGGCGGCCGGCATGGCCACCGCCTCGCCGGTGCGTGCATCGAGCATGAAGGTGTGCGACAGGTTGGGGTCGGTGCTGGCGGTGGCGGCGGTAATGGCGACCACGCCGTTGGGCTCGTCTTCATCGTAGCCGAAGTACTGCATGACCTCGCCGGGGCGATGTTGTTCGGCCTTGAGCACCAGTTGCTGCAGGTCGAGGTGCGGGGTGCCTGCCGGCATTTCACGCAGTTCAGGGGCGTCGCCTAGCAGGTGTTCGAGTTCGTGGTGGAAGATAAGGGGCAGGCCGGTGACT  >  AE015451/424778‑425226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.