New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	2776095 =	35 (0.800)	3 (0.070)	3/318	NT	9.3%	coding (442/1227 nt)	sotB	Probable sugar efflux transporter
	?	AE015451	2776128 =	26 (0.640)					coding (475/1227 nt)	sotB	Probable sugar efflux transporter

GCTGACCCGCAACATCGAACGGCGGCGTCTGCTGCTGTTCGTGTTCCTGGTGTTCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGCCGCTGCTGCCCAGCCAGAACTCCGGTTCGTTGCGCAGCCTGCCGGTCCTGTTCAAGCG  >  AE015451/2775908‑2776321                                                                                                                                                                                                                             |                                                                                                                                                                                                  gCTGACCCGCAACATCGAACGGCGGCGTCTGCTGCTGTTCGTGTTCCTGGTGTTCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTgg                                                                                                                                                                                    <  1:501657/236‑1 (MQ=255)   tGACCCGCAACATCGAACGGCGGCGTCTGCTGCTGTTCGTGTTCCTGGTGTTCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGc                                                                                                                                                                                   >  1:38065/1‑235 (MQ=255)                          cgTCTGCTGCTGTTCGTGTTCCTGGTGTTCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTc                                                                                                                                                                            >  1:24996/1‑219 (MQ=255)                               gctgctgTTCGTGTTCCTGGTGTTCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATc                                                                                                                                                                   <  1:271469/223‑1 (MQ=255)                                                          tGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTa                                                                                                                           >  1:781403/1‑236 (MQ=255)                                                                ccACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTgg                                                                                                                                                                                    <  2:413233/173‑1 (MQ=255)                                                                                             ccggcaatgctcaggaacgtgatacgccagcccagtgcctcgcccaccacacggcccagcgggatgcccaggaccatggccagggtagtaccggtggccagcaggcccagcgccttggcctgctggccACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTgg                                                                                                                                                            <  1:373174‑M2/40‑1 (MQ=255)                                                                                             ccggcaatgctcaggaacgtgatacgccagcccagtgcctcgcccaccacacggcccagcgggatgcccaggaccatggccagggtagtaccggtggccagcaggcccagcgccttggcctgctggccACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTgg                                                                                                                                                            >  2:373172‑M2/129‑168 (MQ=255)                                                                                              cGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGcc                                                                                        <  1:510278/235‑1 (MQ=255)                                                                                                  aTGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGc                                                                                                                                              >  1:627197/1‑177 (MQ=255)                                                                                                           gaacgtgatacgccagcccagtgcctcgcccaccacacggcccagcgggatgcccaggaccatggccagggtagtaccggtggcgagcaggcccagcgccttggcctgctggccACCGGTACTACCCTGGCCATGGTCCTgg                                                                                                                                                                        <  2:36227‑M2/28‑1 (MQ=255)                                                                                                                                cTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGGATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCACATcgctgccg                                                          >  1:97705/1‑231 (MQ=255)                                                                                                                                      cATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATc                                                                                                                         <  1:282193/162‑1 (MQ=255)                                                                                                                                      cATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATc                                                                                                                         >  2:282191/1‑162 (MQ=255)                                                                                                                                           ggTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGCCGCTGCTGCccagcc                                             >  2:507315/1‑233 (MQ=255)                                                                                                                                           ggTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGCGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTgtggtg                                                                                                                                                 >  2:645735/1‑133 (MQ=255)                                                                                                                                                tCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGCCgctgct                                                     >  2:764510/1‑220 (MQ=255)                                                                                                                                                    ccagcgggatgcccaggaccatggccagggtagtaccggtGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCgg                                                                                                     >  2:373172‑M1/41‑168 (MQ=255)                                                                                                                                                    ccagcgggatgcccaggaccatggccagggtagtaccggtGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCgg                                                                                                     <  1:373174‑M1/128‑1 (MQ=255)                                                                                                                                                       cATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATc                                                                      >  2:647384/1‑196 (MQ=255)                                                                                                                                                           aCCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTgg                                                                                                                                                                                    <  2:183511/82‑1 (MQ=255)                                                                                                                                                            ccGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGc                                                                                         <  1:100776/172‑1 (MQ=255)                                                                                                                                                            ccGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGCCGCTGCTGCCCCGCCAGAACTCCGGTTCGTTgcgc                         >  1:64792/1‑236 (MQ=255)                                                                                                                                                            ccGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGATGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCCCCATGCTTTGCCTGATCAAATcgc                                                               >  1:65255/1‑198 (MQ=255)                                                                                                                                                             cGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCCAGCGCCTTGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTgg                                                                                                                                                                        <  2:188824/92‑1 (MQ=37)                                                                                                                                                                ccaggaccatggccagggtagtaccggtGGCCAGCAGGCCAAGGCGCTGGGCCTGCTCGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTc                                                                                                                   <  2:36227‑M1/114‑1 (MQ=255)                                                                                                                                                                 tCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGtactac                                                                                                                                                                                         <  1:512829/71‑1 (MQ=255)                                                                                                                                                                 tCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGtactac                                                                                                                                                                                         >  2:512827/1‑71 (MQ=255)                                                                                                                                                                   gCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATcgctgcc                                                           <  1:89439/195‑1 (MQ=255)                                                                                                                                                                      ggCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGCCGCTGCTGCCCAGCCAGaa                                         >  1:102082/1‑210 (MQ=255)                                                                                                                                                                             cGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGCCGCTGCTGCCCAGCCAGAACTCCGGTTCGTTGCGCAGc                      <  1:453824/222‑1 (MQ=255)                                                                                                                                                                                    ggccgccGGGCCAGCAGGCCAAGCCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATcgctgccgctgc                                                      <  2:102081/182‑1 (MQ=255)                                                                                                                                                                                            GGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCctgct                                                                       <  2:657335‑M1/158‑3 (MQ=255)                                                                                                                                                                                                AGCAGGCCAAGGCGCTGGGCCAGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATTACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGTCGCTGCTGCCCAGCCAGAAGTCCGGTTCGTTGCGCAGCCTGCCGGTCCTGTTCAAgcg  <  2:764640‑M1/223‑1 (MQ=255)                                                                                                                                                                                                                             |                                                                                                                                                                                                  GCTGACCCGCAACATCGAACGGCGGCGTCTGCTGCTGTTCGTGTTCCTGGTGTTCATTGTCAGCCACTTGCTGTCCTGGCTTTCGCAAAGCTTCGCCATGCTGCTGGTCAGCCGCATCGGCATTGCCCTGGCGCATGCGGTGTTCTGGTCCATCACCGCCTCGCTGGCGGTGCGTGTGGCGCCGCCGGGCCAGCAGGCCAAGGCGCTGGGCCTGCTGGCCACCGGTACTACCCTGGCCATGGTCCTGGGCATCCCGCTGGGCCGTGTGGTGGGCGAGGCACTGGGCTGGCGTATCACGTTCCTGAGCATTGCCGGGGTAGCGCTAGCCACCATGCTTTGCCTGATCAAATCGCTGCCGCTGCTGCCCAGCCAGAACTCCGGTTCGTTGCGCAGCCTGCCGGTCCTGTTCAAGCG  >  AE015451/2775908‑2776321

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.