New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	4861128 =	42 (0.960)	4 (0.110)	4/292	NT	12.0%	intergenic (‑79/+158)	ligA/PP_4275	DNA ligase/putative cell division protein ZipA
	?	AE015451	4861219 =	23 (0.620)					intergenic (‑170/+67)	ligA/PP_4275	DNA ligase/putative cell division protein ZipA

CGAGCTCGGCACGCAGTTCGAGGATACGGGTTTCGGCGGTCATGGGATTTGTCTTCTCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCACCGTTCAGTTCGTGGGCCAGCTTGCGTGCCGCGGCAACCATCACGT  >  AE015451/4861007‑4861437                                                                                                                                                                                                                     |                                                                                                                                                                                                                           cGAGCTCGGCACGCAGTTCGAGGATACGGGTTTCGGCGGTCATGGGATTTGTCTTCTCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGAGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAccga                                                                                                                                                                                                              >  1:762899/1‑227 (MQ=255)                gTTCGAGGATACGGGTTTCGGCGGTCATGGGATTTGTCTTCTCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACg                                                                                                                                                                                       <  1:178752/235‑1 (MQ=255)                    gAGGATACGGGTTTCGGCGGTCATGGGATTTGTCTTCTCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTc                                                                                                                                                                                  <  1:501756/236‑1 (MQ=255)                       gATACGGGTTTCGGCGGTCATGGGATTTGTCTTCTCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCg                                                                                                                                                                                                     >  2:301417/1‑214 (MQ=255)                                           tGGGATTTGTCTTCTCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGAt                                                                                                                                                             >  2:747558/1‑234 (MQ=255)                                                     cttctCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCtt                                                                                                                                                 >  1:194069/1‑236 (MQ=255)                                                            tGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGtt                                                                                                                                          >  1:632898/1‑236 (MQ=255)                                                                aagcaaaAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAACCCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGt                                                                                                                                                         <  1:87011/217‑1 (MQ=255)                                                                 agcaaaAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCtt                                                                                                                                                                       >  1:174448/1‑202 (MQ=255)                                                                      aaGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAg                                                                                                                                                                                           >  2:568541/1‑177 (MQ=255)                                                                                       gggCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGTGGGTTTACCCGCGAAGAGGCTGGCACAGTCAac                                                                                                                                                                                                                <  2:496553/137‑1 (MQ=255)                                                                                            tGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGGGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCa                                                                                                                                                                                            <  1:608852/154‑1 (MQ=255)                                                                                                     aGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGt                                                                                                  >  1:295237/1‑235 (MQ=255)                                                                                                                        cAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGt                                                                               >  1:727087/1‑235 (MQ=255)                                                                                                                                  GCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATAc                                                                                                              <  1:65455‑M1/194‑1 (MQ=255)                                                                                                                                  GCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGGTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGctc                                                                                                                                                 <  1:222136‑M1/159‑2 (MQ=255)                                                                                                                                    GGGGAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGGCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACt                                                                                                                       <  1:128599‑M1/183‑1 (MQ=255)                                                                                                                                            ccgctcctacaggtacatcaccgccctagggcatgtgacgcccttgtgggagcgggtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGgtc                                                             >  2:595841‑M2/74‑233 (MQ=255)                                                                                                                                                       aGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCAc                                               >  1:770336/1‑236 (MQ=255)                                                                                                                                                          gCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTCCCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCGGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTgg                                                               <  1:759774/217‑1 (MQ=255)                                                                                                                                                            gTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAg                                                                            >  1:56695/1‑202 (MQ=255)                                                                                                                                                               acaTGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGGAGAGGCCGGCACAGGCAACGGCGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCGCTTCCGTCTGGCTCTGTCAGCGCTTCGGGGTTAGCGCag                                                                                                                                   >  2:529354/1‑143 (MQ=38)                                                                                                                                                                ccgccctagggcatgtgacgcccttgtgggagcgggtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCACCg                                             <  1:595843‑M2/176‑1 (MQ=255)                                                                                                                                                                   ccctagggcatgtgacgcccttgtgggagcgggtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCa                                                                                                                                                                                            <  2:360778‑M2/33‑1 (MQ=255)                                                                                                                                                                   ccctagggcatgtgacgcccttgtgggagcgggtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCa                                                                                                                                                                                            >  1:360780‑M2/51‑83 (MQ=255)                                                                                                                                                                             ggTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAAc                                                                                                                        >  2:735345/1‑141 (MQ=255)                                                                                                                                                                                 aTGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGGCTGGGCGGTCAGCAc                                                                         >  1:172830/1‑184 (MQ=255)                                                                                                                                                                                  tGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTa                                                                                                    >  1:82137/1‑156 (MQ=255)                                                                                                                                                                                  tGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGGTgg                                                                                         >  1:90673/1‑167 (MQ=255)                                                                                                                                                                                   gTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGgccgct                                                                                                                                                                          >  2:83140/1‑85 (MQ=255)                                                                                                                                                                                             gagcgggtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCACCGTTCAGTTCGTGGGCCAGCTTGCGTGCCGCGGCAAc          <  1:747560‑M2/211‑1 (MQ=255)                                                                                                                                                                                                  ggtttacccgcgaagaggcCGGca                                                                                                                                                                                                                        <  2:758303‑M2/5‑1 (MQ=255)                                                                                                                                                                                                  ggtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCa                                                                                                                                                                                            <  1:639358‑M2/33‑1 (MQ=255)                                                                                                                                                                                                   gtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTg                                                                                     <  2:499330‑M2/136‑1 (MQ=255)                                                                                                                                                                                                   gtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTg                                                                                     >  1:499332‑M2/19‑154 (MQ=255)                                                                                                                                                                                                   gtttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCACCGTTCAGTTCGTGGGCCAGCTTGCGTGCCGCGGCAACCATCAc    >  1:363625‑M2/19‑235 (MQ=255)                                                                                                                                                                                                     ttacccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCACCGTTCAGTTCGTGGGCCAGCTTGCGTGCCGCGGCAACCATCACGt  >  1:374650‑M2/17‑235 (MQ=255)                                                                                                                                                                                                       ccccgcgaagaggcCGGCACAGGCACCGACGATCCCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGc                                                                           <  2:222134‑M2/146‑1 (MQ=255)                                                                                                                                                                                                         ccgcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTc                                                                                       <  2:563996‑M2/134‑1 (MQ=255)                                                                                                                                                                                                           gcgaagaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCa                                                                          >  2:372029‑M2/11‑157 (MQ=255)                                                                                                                                                                                                            cgaaggggcCGGCACAGGCAACGACGATACCCGAAc                                                                                                                                                                                                  <  2:523137‑M2/27‑1 (MQ=255)                                                                                                                                                                                                              cagaggcCGGCACAGGCAACGACGATACCCGATCCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGGGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTc                                                                                              <  2:374424‑M2/127‑1 (MQ=255)                                                                                                                                                                                                                gaggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCACCGTTCAGTTCGTGGGCCAGCTTGCGTGCCGCGGCAACCATCa     >  2:726200‑M2/6‑221 (MQ=255)                                                                                                                                                                                                                 aggcCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGTATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTTGTGGTCGGTGGTCTGGGc                                                                                  >  1:244009‑M2/5‑143 (MQ=255)                                                                                                                                                                                                                     |                                                                                                                                                                                                                           CGAGCTCGGCACGCAGTTCGAGGATACGGGTTTCGGCGGTCATGGGATTTGTCTTCTCTTGCAAAGCAAAAGAGCAGCTTGGGGTGGTGCGTGGGGTTCCAGGTGTCTGTTGACTGTGCCAGCCTCTTCGCGGGTAAACCCGCTCCCACAAGGGCGTCACATGCCCTAGGGCGGTGATGTACCTGTAGGAGCGGGTTTACCCGCGAAGAGGCCGGCACAGGCAACGACGATACCCGAACCGGCCAGCACGCCCTCAGGCTGCTCTTTCGTCTGGATCTGTCAGCGCTTCTGGGTTAGCGCACGGCGCTCGAACTCGACGATACGCTGGCGGTAGTGCTCGATGGTCTGGGCGGTCAGCACGCTGCGCTGGTCGTCCTTGAGCTCACCGTTCAGTTCGTGGGCCAGCTTGCGTGCCGCGGCAACCATCACGT  >  AE015451/4861007‑4861437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.