New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	4338572 =	26 (1.010)	4 (0.160)	4/302	NT	12.0%	coding (244/294 nt)	PP_5632	enzyme subunit involved in allo‑isoleucine metabolism
	?	AE015451	4338688 =	34 (1.390)					coding (128/294 nt)	PP_5632	enzyme subunit involved in allo‑isoleucine metabolism

GGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGG  >  AE015451/4338351‑4338795                                                                                                                                                                                                                              |                                                                                                                                                                                                                                ggCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCAt                                                                                                                                                                                                                    <  1:132464/235‑1 (MQ=255)                                          attttatggtataccatcatacacaaatatcatcgtaacaggagagctgcacatgaaggcctactggatagcccatgtggacgtgaccgaccccgagcaataccagcagtacacccagcgcgccccagctgccttcaaggcattcggcggtcgcttcctggcccgtggcgggcgtagtgaGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCggtaacaggcctgtctctt                                                                                                                                                                           <  1:429339‑M1/55‑10 (MQ=255)                                                   ccTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACg                                                                                                                                                                   >  1:340558/1‑234 (MQ=255)                                                    ataccatcatacacaaatatcatcgtaacaggagagctgcacatgaaggcctactggatagcccatgtggacgtgaccgaccccgagcaataccagcagtacacccagcgcgccccagctgccttcaaggcattcggcggtcgcttcctggcccgtggcgggcgtagtgaGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAAt                                                                                                                                                                >  1:461282‑M1/171‑236 (MQ=255)                                                            gTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGt                                                                                                                                                                                                          <  1:244596/186‑1 (MQ=255)                                                                  aaatatcatcgtaacaggagagctgcacatgaaggcctactggatagcccatgtggacgtgaccgaccccgagcaataccagcagtacacccagcgcgccccagctgccttcaaggcattcggcggtcgcttcctggcccgtggcgggcgtagtgaGGCGATGGCTGCATGCCTCTTGGTACAGcgcg                                                                                                                                                                                                  >  2:429335‑M1/157‑188 (MQ=255)                                                                     ttCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGc                                                                                                                                                                                                                      <  2:176380/165‑1 (MQ=255)                                                                                     aGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTc                                                                                                                                                         <  2:283182/210‑1 (MQ=255)                                                                                            aGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGt                                                                                                                                                                                           <  2:412263/169‑1 (MQ=255)                                                                                                     tGAATCTGCCAGCGGTGCGGAAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAg                                                                                                                                     <  1:55236/214‑1 (MQ=255)                                                                                                           tGCCAGCGGTGCGGCAGGTGCGGCCCTTTCGCGGGTACACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGa                                                                                                                                                                                                                             <  2:352836/120‑1 (MQ=255)                                                                                                            gCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGa                                                                                                                                                       <  1:449161/189‑1 (MQ=255)                                                                                                              cAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGaa                                                                                                                                                            <  1:515288/182‑1 (MQ=255)                                                                                                                   gTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTAcgcccgc                                                                                                 <  1:426312/236‑1 (MQ=255)                                                                                                                    tGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGAt                                                                                                                                                      <  2:228960/182‑1 (MQ=255)                                                                                                                        gCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGc                                                                                                                                                                                                                      <  1:191297/114‑1 (MQ=255)                                                                                                                            gTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTc                                                                                                                                                         <  2:374679/171‑1 (MQ=255)                                                                                                                               cGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTAcgcccgc                                                                                                 <  1:384266/224‑1 (MQ=255)                                                                                                                               cGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAACGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGt                                                                                                                                                                                           <  2:127668/134‑1 (MQ=255)                                                                                                                                 gCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCATCCTTCGCCACCCCTTgg                                                                                                                                                                                                                                >  2:227285/1‑95 (MQ=255)                                                                                                                                    cTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCACGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTAcgcccgc                                                                                                 <  1:474596/219‑1 (MQ=255)                                                                                                                                                            ccagcgcgccccagctgccttcaaggcattcggcggtcgcttcctggcccgtggcgggcgtagtgaGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGAt                                                                                                                                                      >  1:71841‑M1/67‑142 (MQ=255)                                                                                                                                                                 gCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGgc                                                   <  1:356119/236‑1 (MQ=255)                                                                                                                                                                   aaaTCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGgcgc                                                 <  1:416631/236‑1 (MQ=255)                                                                                                                                                                               gggCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGa                                                                                                                                                       <  2:275841/122‑1 (MQ=255)                                                                                                                                                                               gggCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGa                                                                                                                                                       >  1:275845/1‑122 (MQ=255)                                                                                                                                                                                 gCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCCCCACGGGCCAGGAAGCGAccgcc                                                                           >  1:498895/1‑196 (MQ=255)                                                                                                                                                                                   tCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCt                                                                                                                                                                            <  1:264427/97‑1 (MQ=255)                                                                                                                                                                                   tCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCt                                                                                                                                                                            >  2:264423/1‑97 (MQ=255)                                                                                                                                                                                             ccACAATGATCACCCCAGCCGTCGCCACGCCTTGGCGATGGCTGCAGGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGgcg                                                  <  1:380810/209‑1 (MQ=255)                                                                                                                                                                                                             aGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGggtcggt               >  2:259199/1‑228 (MQ=255)                                                                                                                                                                                                                  tCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATgg  >  1:168481/1‑236 (MQ=255)                                                                                                                                                                                                                          ccggGGCGATGGCCGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTa                                                                                                                                >  2:62532‑M1/5‑102 (MQ=255)                                                                                                                                                                                                                           cTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTAct                                     >  2:159362/1‑192 (MQ=255)                                                                                                                                                                                                                            ttGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTCCGAATCGAACGCGag                                                                                                                                                      >  2:62994/1‑77 (MQ=255)                                                                                                                                                                                                                              |                                                                                                                                                                                                                                GGCAACGAACTTAAAGTGCATCTGCAGTGAGGCGATGAGCAATAGACCCGCCTGGGCGGGTCTTTTGCTTCTATTTGCGGAAACAGCCCGCAGGCTTCAATGAATCTGCCAGCGGTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGG  >  AE015451/4338351‑4338795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.