New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	4338572 =	26 (1.010)	4 (0.160)	4/302	NT	12.0%	coding (244/294 nt)	PP_5632	enzyme subunit involved in allo‑isoleucine metabolism
	?	AE015451	4338688 =	34 (1.390)					coding (128/294 nt)	PP_5632	enzyme subunit involved in allo‑isoleucine metabolism

GTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCACCATGGCTT  >  AE015451/4338465‑4338900                                                                                                                                                                                                                                |                                                                                                                                                                                                                     gTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTAcgcccgc                                                                                                                                                                                                          <  1:426312/236‑1 (MQ=255)             cGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTAcgcccgc                                                                                                                                                                                                          <  1:384266/224‑1 (MQ=255)                  cTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCACGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTAcgcccgc                                                                                                                                                                                                          <  1:474596/219‑1 (MQ=255)                                               gCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGgc                                                                                                                                                            <  1:356119/236‑1 (MQ=255)                                                 aaaTCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGgcgc                                                                                                                                                          <  1:416631/236‑1 (MQ=255)                                                               gCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCCCCACGGGCCAGGAAGCGAccgcc                                                                                                                                                                                    >  1:498895/1‑196 (MQ=255)                                                                           ccACAATGATCACCCCAGCCGTCGCCACGCCTTGGCGATGGCTGCAGGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGgcg                                                                                                                                                           <  1:380810/209‑1 (MQ=255)                                                                                           aGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGggtcggt                                                                                                                        >  2:259199/1‑228 (MQ=255)                                                                                                tCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATgg                                                                                                           >  1:168481/1‑236 (MQ=255)                                                                                                         cTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTAct                                                                                                                                              >  2:159362/1‑192 (MQ=255)                                                                                                              CGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGc                                                                                             <  1:174846‑M1/236‑1 (MQ=255)                                                                                                                 TGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCt                                                                                           <  1:175455‑M1/235‑1 (MQ=255)                                                                                                                      gCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGACTCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCCCGTccc                                                                                                                >  1:315228/1‑208 (MQ=255)                                                                                                                            ctctTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCAt                                                                                       <  2:340554/228‑1 (MQ=255)                                                                                                                                        cgcgGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGggtcggtc                                                                                                                       <  2:168477/184‑1 (MQ=255)                                                                                                                                          cgGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCAt                                                                                       >  1:376019/1‑214 (MQ=255)                                                                                                                                          cgGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCAt                                                                                       <  2:376015/214‑1 (MQ=255)                                                                                                                                           ggAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTc                                                                                                                  >  2:258110/1‑186 (MQ=255)                                                                                                                                           ggAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGc                                                                                                         >  1:314604/1‑195 (MQ=255)                                                                                                                                           ggAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACgat                                                                  >  2:268837/1‑234 (MQ=255)                                                                                                                                                ggTAACAGGCGACGGCCTGCGCGTCCGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCGGGGTGTACTGCTGGTGCTGCTCGGggt                                                                                                                            >  2:239877/1‑171 (MQ=255)                                                                                                                                                  tAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTtgt                                                         <  1:259203/236‑1 (MQ=255)                                                                                                                                                    atcgagttcgattcgtacgcgcaggccgtcgcctgttaccgctccgcgctgtaccaagaggcatgcagccatcgccTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTgg                                                                                                                                                     >  1:71841‑M2/77‑142 (MQ=255)                                                                                                                                                    aCAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATAttt                                                           <  2:175451/232‑1 (MQ=255)                                                                                                                                                              attcgtacgcgcaggccgtcgcctgttaccgctccgcgctgtaccaagaggcatgcagccatcgccTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGtat                                             >  1:461282‑M2/67‑236 (MQ=255)                                                                                                                                                                         aagagacaggcctgttaccgctccgcgctgtaccaagaggcatgcagccatcgccTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGTATACCATAAAAt                                   <  1:429339‑M2/180‑1 (MQ=255)                                                                                                                                                                            aTCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTa                                                      <  1:497128/213‑1 (MQ=255)                                                                                                                                                                                       gcaccacACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCgg                                                                                                                               <  2:315224/128‑1 (MQ=255)                                                                                                                                                                                           cacaCTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCCAACTACGCCGGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACgatga                                                                <  1:315775/188‑1 (MQ=255)                                                                                                                                                                                            ccacTACGCTGGGGCGTAGCCCTCCCTTCCATCGCCTCGCTCCGCCCGCCACGGGCCAGGCAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATcc                                                                                                    <  2:498891/150‑1 (MQ=255)                                                                                                                                                                                                tccGCTGAGGCGTAGCCCGCCCTTCCAGCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTa                                                      <  1:1836/191‑1 (MQ=255)                                                                                                                                                                                                tACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGata                                                              <  1:346115/185‑1 (MQ=255)                                                                                                                                                                                                cgcgctgtaccaagaggcatgcagccatcgccTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATAttt                                                           >  2:429335‑M2/33‑188 (MQ=255)                                                                                                                                                                                                 aCGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCa           <  2:479976/235‑1 (MQ=255)                                                                                                                                                                                                   gCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCAcc         <  1:159366/235‑1 (MQ=255)                                                                                                                                                                                                       aGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACgatga                                                                <  2:374771/176‑1 (MQ=255)                                                                                                                                                                                                        ggCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGggtcggtc                                                                                                                       >  1:450973/1‑120 (MQ=255)                                                                                                                                                                                                        ggCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGggtcggtc                                                                                                                       <  2:450969/120‑1 (MQ=255)                                                                                                                                                                                                          cGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCACCATGGCtt  >  1:419356/1‑235 (MQ=255)                                                                                                                                                                                                               ccctccctTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGAccgc                                                                                                                                                                                     >  1:401484/1‑51 (MQ=255)                                                                                                                                                                                                                    cctTCCATCGCCTCACTACGGCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCa           <  2:128345/216‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                     GTGCGGCAGGTCCGGCCCTTTCGCGGGTAAACCCGCGAAAGGGCCGGCAAATCCTGCTCAGGGCTCAAACCCTTCCACAATGATCACCTCAGCCTTCGCCACCCCTTGGCGATGGCTGCATGCCTCTTGGTACAGCGCGGAGCGGTAACAGGCGACGGCCTGCGCGTACGAATCGAACTCGATCACCACACTACGCTGAGGCGTAGCCCTCCCTTCCATCGCCTCACTACGCCCGCCACGGGCCAGGAAGCGACCGCCGAATGCCTTGAAGGCAGCTGGGGCGCGCTGGGTGTACTGCTGGTATTGCTCGGGGTCGGTCACGTCCACATGGGCTATCCAGTAGGCCTTCATGTGCAGCTCTCCTGTTACGATGATATTTGTGTATGATGGTATACCATAAAATTCACCCCACCACAGTGAGCGTGCACCATGGCTT  >  AE015451/4338465‑4338900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.