New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	734319 =	27 (0.470)	7 (0.130)	6/320	NT	22.1%	coding (998/1296 nt)	ndh	NADH dehydrogenase
	?	AE015451	734381 =	24 (0.440)					coding (936/1296 nt)	ndh	NADH dehydrogenase

CGCCAGCCAGCCTTCCAGCTTCACGCTGCCCATCAGGTTACCCATCAGGTTGCCCACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCTGGATCACTTCACCGTCCTTGGTTTTCAGGCCGTCCTCGGTCACTTCACTGACTGCGGCGTTGGTCATCACCTTCACCCCGA  >  AE015451/734147‑734610                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      cgcCAGCCAGCCTTCCAGCTTCACGCTGCCCATCAGGTTACCCATCAGGTTGCCCACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAg                                                                                                                                                                                                                                       >  1:551747/1‑235 (MQ=255) cgcCAGCCAGCCTTCCAGCTTCACGCTGCCCATCAGGTTACCCATCAGGTTGCCCACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGt                                                                                                                                                                                                                                      >  1:223131/1‑236 (MQ=255)                                aTCAGGTTACCCATCAGGTTGCCCACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGa                                                                                                                                                                                                                          <  2:528480/217‑1 (MQ=255)                                  gaaggtgtgggcagccggtatccgcgcgccaggtttcctcaaggacatcgatggcctggaaaccaaccgcatcaaccagctggtcgtgcgccctaccctgcaaaccacccgcgacgacaacatcttcgccttcggtgactgcgccgcctgcccgcaacccggcagcgatcgtaacgtgccgccgcgcgcacaggcggcgcaGTCACCGAAGGCGAAGAtgttg                                                                                                                                                                                                                  >  1:76445‑M2/202‑223 (MQ=255)                                           cATCAGGTTGCCCACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAgg                                                                                                                                                                                             >  1:627096/1‑235 (MQ=255)                                                  ggtatccgcgcgccaggtttcctcaaggacatcgatggcctggaaaccaaccgcatcaaccagctggtcgtgcgccctaccctgcaaaccacccgcgacgacaacatcttcgccttcggtgactgcgccgcctgcccgcaacccggcagcgatcgtaacgtgccgccgcgcgcacaggcggcgcaGTCACCGAAGGCGAAGATGTTGTCGTCGCGggt                                                                                                                                                                                                       <  2:76443‑M2/33‑1 (MQ=255)                                                       cACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGCtgttg                                                                                                                                                                                                                  >  1:213567/1‑202 (MQ=255)                                                       cACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGa                                                                                                                                                                                >  1:177838/1‑236 (MQ=255)                                                                  ggcgcGGCAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCa                                                                                                                                                                                                                                    <  2:702415/172‑1 (MQ=255)                                                                         cagcgacaCCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGgc                                                                                                                                                                                      >  2:314495/1‑212 (MQ=255)                                                                          agcgacaCCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGa                                                                                                                                                                    <  1:314498/229‑1 (MQ=255)                                                                                            gaaaccaaccgcatcaaccagctggtcgtgcgccctaccctgcaaacgacccgcgacgacaacatcttcgccttcggtgactgcgccgcctgcccgcaacccggcagcgatcgtaacgtgccgccgcgcgcacaggcggcgcaGTCACCGAAg                                                                                                                                                                                                                              <  1:1031522‑M2/10‑1 (MQ=255)                                                                                                             cGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGTTGGTTGATGCGGTTGGTTTCCAGGc                                                                                                                                                  >  1:250206/1‑212 (MQ=255)                                                                                                             cGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATg                                                                                                                                          >  1:989030/1‑220 (MQ=255)                                                                                                                cGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAg                                                                                                                                                                                                                        >  2:446594/1‑139 (MQ=255)                                                                                                                cGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCggttggtt                                                                                                                                                         >  2:654791/1‑202 (MQ=255)                                                                                                                                  cAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCATCTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTCGAGGAAACCTGGCGCGCGGATACCGGCTGc                                                                                                       <  1:600050/234‑1 (MQ=255)                                                                                                                                          ccTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCtt                                                                                              >  1:193777/1‑235 (MQ=255)                                                                                                                                          ccTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGGTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGcgcgcg                                                                                                                   >  1:209342/1‑214 (MQ=255)                                                                                                                                           cTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAg                                                                                                                                                    >  2:837852/1‑180 (MQ=255)                                                                                                                                            acccgcgacgacaacatcttcgccttcggtgacTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATAcc                                                                                                             <  2:76443‑M1/185‑1 (MQ=255)                                                                                                                                               cgcgacgacaacatcttcgccttcggtgacTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTGAcc                                                                                                                                                                                                                                  <  2:560294‑M1/68‑1 (MQ=255)                                                                                                                                                     tGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCt                                                                                   <  2:564033/235‑1 (MQ=255)                                                                                                                                                       caacatcttcgccttcggtgacTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTc                                                                                             >  1:76445‑M1/23‑223 (MQ=255)                                                                                                                                                        gcCAGCATCGAAGCCTGCTGGTGCGCCGCCTGGGCGCGCGGCGGCCCGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAAcc                                                                                                                            <  2:273732/191‑1 (MQ=255)                                                                                                                                                                   cttcggtgacTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTCGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTc                                                                                                                                                       <  1:1031522‑M1/143‑1 (MQ=255)                                                                                                                                                                     ccTGCTGGGGCGCCGCCTGTGCGCGCGGCGGCACGTTACGAGCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCTGGa                                                                                <  1:148952/222‑1 (MQ=255)                                                                                                                                                                       ggtcactgcgccgcctgcccgcaacccggcagcgatcgtaacgtgccgccgcgcgcacaggcggcgcaGTCACCGAAGGCGAAGATGTTGTCGTcgcg                                                                                                                                                                                                          <  2:560294‑M2/30‑1 (MQ=255)                                                                                                                                                                            gTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGc                                                                                                       <  2:1011735/192‑1 (MQ=255)                                                                                                                                                                                             gcggcgGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCTGGATCACTTCACCGTCCTTGGTTTTCa                                                        <  2:364479/222‑1 (MQ=255)                                                                                                                                                                                              cggcggCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATAc                                                                                                              <  2:642893/167‑1 (MQ=255)                                                                                                                                                                                               cccggcagcgatcgtaacgtgccgccgcgcgcacaggcggcgcaGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCt                                                                                   >  1:81266‑M2/45‑193 (MQ=255)                                                                                                                                                                                               cccggcagcgatcgtaacgtgccgccgcgcgcacaggcggcgcaGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCt                                                                                   <  2:81264‑M2/149‑1 (MQ=255)                                                                                                                                                                                                   gcagcggtcgtaacgtgccgccgcgcgcacaggcggcgcaGTCACCGAAGGCGAAGATGTTGTCGTCGCGggt                                                                                                                                                                                                       <  2:308682‑M2/33‑1 (MQ=255)                                                                                                                                                                                                      cGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCTGGATCACTTCACCGTCCTTGGTTTTCAGGCCGTCCTCg                                             <  1:897235/224‑1 (MQ=255)                                                                                                                                                                                                             aTCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCTGGATCACTTCACCGTCCTTGGTTTTCAGGCCGTCCTCGGtcacttc                                     <  2:177836/225‑1 (MQ=255)                                                                                                                                                                                                                    ccGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGa                                                                                                                 >  2:922479/1‑142 (MQ=255)                                                                                                                                                                                                                    ccGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGCTGTCGTCGCGGGGGGTTTGCAGGGTAGGGCGCACGAACAGCTGGTTGTTGCg                                                                                                                                                                >  2:155192/1‑95 (MQ=255)                                                                                                                                                                                                                                      gcgcaGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCTGGATCACTTCACCGTCCTTGGTTTTCAGGCCGTCCTCGGTCACTTCACTGACTGCGGCGTTGGTCATCACCTTCACCCCGa  >  1:309572‑M2/6‑235 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                      CGCCAGCCAGCCTTCCAGCTTCACGCTGCCCATCAGGTTACCCATCAGGTTGCCCACCGCCGAGAAGCGCGACAGCGACACCAGCGAGCCATAGTCCTTGTACTCGTACGTCGGCAGGGCCTTGTTCTCCAGGCGCGCCTTCAGGCTTTGCGCCAGCATCGAAGCCTGCTGGTGCGCCGCCTGTGCGCGCGGCGGCACGTTACGATCGCTGCCGGGTTGCGGGCAGGCGGCGCAGTCACCGAAGGCGAAGATGTTGTCGTCGCGGGTGGTTTGCAGGGTAGGGCGCACGACCAGCTGGTTGATGCGGTTGGTTTCCAGGCCATCGATGTCCTTGAGGAAACCTGGCGCGCGGATACCGGCTGCCCACACCTTCAGGCTGGCCTGGATCACTTCACCGTCCTTGGTTTTCAGGCCGTCCTCGGTCACTTCACTGACTGCGGCGTTGGTCATCACCTTCACCCCGA  >  AE015451/734147‑734610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.