New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 2388103	27 (0.470)	3 (0.050)	3/326	NT	10.2%	coding (863/1023 nt)	pyrD	Dihydroorotate dehydrogenase (quinone)
	?	AE015451	= 2388114	27 (0.490)					coding (874/1023 nt)	pyrD	Dihydroorotate dehydrogenase (quinone)

ACGAAGAAACGGCGCTGGTGGCCGCTGCGCTGATGGAGTCGGGCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCT  >  AE015451/2387908‑2388276                                                                                                                                                                                                               |                                                                                                                                                                   acgaAGAAACGGCGCTGGTGGCCGCTGCGCTGATGGAGTCGGGCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGgccg                                                                                                                                        <  1:641975/235‑1 (MQ=255) acgaAGAAACGGCGCTGGTGGCCGCTGCGCTGATGGAGTCGGGCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGgccg                                                                                                                                        <  1:361038/235‑1 (MQ=255)           ggggcTGGTGGCCGCTGCGCTGATGGAGTCGGGCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTgccgc                                                                                                                                                             <  1:170086/201‑1 (MQ=255)                                 aTGGAGTCGGGCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGa                                                                                                        <  1:777370/235‑1 (MQ=255)                                     aGTCGGGCATGGATGCGGTGATCGCCACCAACACCTCGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGgccgcc                                                                                                                                      <  1:12044/201‑1 (MQ=255)                                     aGTCGGGCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTg                                                                                                   <  1:839708/236‑1 (MQ=255)                                         gggCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTAcc                                                                                                                                                <  2:720701/187‑1 (MQ=255)                                             aTGGATGCGGTGATCGCCGCCCACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGa                                                                                                        <  1:337753/223‑1 (MQ=255)                                                              caccaACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCAttgccgcccagctcacctgccagcaccttgacgatatgggtgctcttctccagcaccgg                                                                                                         >  1:567106‑M2/1‑146 (MQ=255)                                                              caccaACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCt                                                                          <  1:406622/236‑1 (MQ=255)                                                                                ccGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAg                                                                                                       <  2:498568/189‑1 (MQ=255)                                                                                  gTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCg                                                                                                         <  1:914129/185‑1 (MQ=255)                                                                                                gCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGcc                                                                                                     <  2:1029728/175‑1 (MQ=255)                                                                                                gCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACAGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGcc                                                                                                     >  1:1029731/1‑175 (MQ=255)                                                                                                   tggCCGTACGGGGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGa                                                                                                        <  2:729677/167‑1 (MQ=255)                                                                                                           cGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATcgcg                              <  1:493536/235‑1 (MQ=255)                                                                                                                   aGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGa                                                                                                        >  1:554504/1‑153 (MQ=255)                                                                                                                   aGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGa                                                                                                        <  2:554501/153‑1 (MQ=255)                                                                                                                   aGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTc                                                     >  2:493533/1‑204 (MQ=255)                                                                                                                        ggcggcCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGt                 <  1:694499/235‑1 (MQ=255)                                                                                                                         gtggccgGTCTGGCGCACCGGTGCTGGTGAAGCCCAGCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGACAAGTTGCCGTTCAttgccgc                                                                                                                                                             >  2:918980‑M2/3‑87 (MQ=255)                                                                                                                           ggcCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCAttgccgcccagctcacctgccagcaccttgacgatatgggtgctcttctccag                                                                                                               <  1:698973‑M2/138‑54 (MQ=255)                                                                                                                           ggcCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCAttgccgcccagctcacctgccagcaccttgacgatatgggtgctcttctccag                                                                                                               >  2:698970‑M2/1‑85 (MQ=255)                                                                                                                           ggcCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTc                                                     >  1:449359/1‑196 (MQ=255)                                                                                                                           ggcCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTc                                                     <  2:449356/196‑1 (MQ=255)                                                                                                                            gcCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCa            <  1:646122/236‑1 (MQ=255)                                                                                                                                         cccGGTGCTGGAGAAGAGCCCCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCt  <  1:328214/232‑1 (MQ=255)                                                                                                                                                                atatCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAg                                                                    <  2:152647/144‑1 (MQ=255)                                                                                                                                                                         aGGTGCGGGCGGGTGAGCTGGGCGGCAGGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGcc                      <  2:192211/181‑1 (MQ=255)                                                                                                                                                                                ggCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGTGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGa                                        <  2:504565/156‑1 (MQ=255)                                                                                                                                                                                        aGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGACGGCCGCCAGGCGGCGGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCg                                                               <  2:654808/125‑1 (MQ=255)                                                                                                                                                                                                ggcAAGTTGCCGATCATGGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGCGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCt                                    <  2:1042740/144‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                   ACGAAGAAACGGCGCTGGTGGCCGCTGCGCTGATGGAGTCGGGCATGGATGCGGTGATCGCCACCAACACCACGCTGGGCCGTGAAGGTGTCGAAGCATTGCCGTACGGTGGCGAGGCGGGCGGCCTGTCGGGCGCACCGGTGCTGGAGAAGAGCACCCATATCGTCAAGGTGCTGGCAGGTGAGCTGGGCGGCAAGTTGCCGATCATTGCCGCCGGGGGCATTACCGAAGGCCGCCATGCTGCCGAGAAGATCGCGGCCGGGGCGAGCCTGGTGCAGATATACTCGGGCTTCATCTACAAAGGGCCGGCGCTGATTCGCGAAGCGGTGGATGCTATCGCGGCAATGCCGCGGTGACCAGCCCGCCCCT  >  AE015451/2387908‑2388276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.