New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	3921023 =	38 (0.950)	3 (0.080)	3/318	NT	9.1%	coding (743/1689 nt)	acs	Butyrate‑‑CoA ligase
	?	AE015451	3921136 =	24 (0.630)					coding (630/1689 nt)	acs	Butyrate‑‑CoA ligase

CCACCGCCGGGGTTTCTGGCCTGGCACGCATTGCAGGCCCGTGCCGAGGTTGTCAGCGGCGAGGCCCTGGCCGCGCGCCAGGCACAACTGCGTTGCGACGACCCGATCAACATCCAGTACACCTCAGGCACCACCGGTTTCCCCAAGGGGGCCACCCTTAGCCACAGCAATATCCTCAACAACGGGTACATGGTCGGTGAAAGCCTGGGCCTTACCGAGCACGATCGGCTGGTGGTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/3921261‑3921023 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtggtgccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCAGGCCCTGCAGCATG  >  AE015451/3921136‑3921364                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      CCACCGCCGGGGTTTCTGGCCTGGCACGCATTGCAGGCCCGTGCCGAGGTTGTCAGCGGCGAGGCCCTGGCCGCGCGCCAGGCACAACTGCGTTGCGACGACCCGATCAACATCCAGTACACCTCAGGCACCACCGGTTTCCCCAAGGGGGCCACCCTTAGCCACAGCAATATCCTCAACAACGGGTACATGGTCGGTGAAAGCCTGGGCCTTACCGAGCACGATCGGCTGGTGG                                                                                                                                                                                                                                           >  1:741870/1‑235                                                                              CCAGGCACAACTGCGTTGCGACGACCCGATCAACATCCAGTACACCTCAGGCACCACCGGTTTCCCCAAGGGGGCCACCCTTAGCCACAGCAATATCCTCAACAACGGGGACATGGTCGGTGAAAGCCTGGGCCTTACCGAGCACGCTCGGCTGGgg                                                                                                                                                                                                                                            >  2:163465/1‑155                                                                                                                                                                           TATCCTCAACAACGGGTACATGGTCGGTGAAAGCCTGGGCCTTACCGAGCACGATCGGCTGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCA                                                                                              >  2:680452/1‑206                                                                                                                                                                                                       GAAAGCCTGGGCCTTACCGAGCACGATCGGCTGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGC                                                                                                                                    >  1:558711/1‑140                                                                                                                                                                                                       GAAAGCCTGGGCCTTACCGAGCACGATCGGCTGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGC                                                                                                                                    <  2:558710/140‑1                                                                                                                                                                                                                                       GGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGGTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGGTGGCCAGGCCCGGGAGCAGGCCCT          >  1:240679/1‑230                                                                                                                                                                                                                                          GGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCAGGCCCTGCAGCATG  <  1:747343/235‑1                                                                                                                                                                                                                                              CCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCCGGCCCT          >  1:245219/1‑223                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      CCACCGCCGGGGTTTCTGGCCTGGCACGCATTGCAGGCCCGTGCCGAGGTTGTCAGCGGCGAGGCCCTGGCCGCGCGCCAGGCACAACTGCGTTGCGACGACCCGATCAACATCCAGTACACCTCAGGCACCACCGGTTTCCCCAAGGGGGCCACCCTTAGCCACAGCAATATCCTCAACAACGGGTACATGGTCGGTGAAAGCCTGGGCCTTACCGAGCACGATCGGCTGGTGGTGCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/3921261‑3921023 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggtggtgccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCAGGCCCTGCAGCATG  >  AE015451/3921136‑3921364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.