New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	3921023 =	38 (0.950)	3 (0.080)	3/318	NT	9.1%	coding (743/1689 nt)	acs	Butyrate‑‑CoA ligase
	?	AE015451	3921136 =	24 (0.630)					coding (630/1689 nt)	acs	Butyrate‑‑CoA ligase

AAGGCATCGCTGGGGTAAATCAGTGCGCTGCCGTGTGTCATGCAGCCAAGGTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCAGGCCCTGCAGCATG  >  AE015451/3920933‑3921364                                                                                                                                                                                                            |                                                                                                                                                                                                                                     aaGGCATCGCTGGGGTAAATCAGTGCGCTGCCGTGTGTCATGCAGCCAAGGTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCt                                                                                                                                                                                                                                      <  2:734020/204‑1 (MQ=255)                        tGCGCTGCCGTGTGTCATGCAGCCAAGGTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGcgcgcg                                                                                                                                                                                <  1:669160/235‑1 (MQ=255)                                            aGCCAAGGTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGct                                                                                                                                                                                                      >  1:54343/1‑192 (MQ=255)                                            aGCCAAGGTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGGGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGt                                                                                                                                                                                               >  2:248502/1‑200 (MQ=255)                                              ccAAGGTTGGCCATGACAATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGt                                                                                                                                                                                                                      <  2:54343/175‑1 (MQ=255)                                                  ggTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCt                                                                                                                                                                                                                                      <  2:299716/155‑1 (MQ=255)                                                  ggTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCg                                                                                                                                                     <  1:134636/236‑1 (MQ=255)                                                               cATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGgtcgt                                                                                                                                                                                                          >  2:309679/1‑170 (MQ=255)                                                                    cGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGc                                                                                                                                                                        >  2:14612/1‑199 (MQ=255)                                                                    cGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAAt                                                                                                                                      >  1:627476/1‑233 (MQ=255)                                                                          aGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTgccag                                                                                                                             >  1:319999/1‑236 (MQ=255)                                                                                      gcaccggcaccACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCg                                                                                                                                                                                                                >  2:306359/1‑141 (MQ=255)                                                                                         ccggcaccACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAAcc                                                                                                                   <  2:635867/231‑1 (MQ=255)                                                                                               CCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTgg                                                                                                         >  1:741870‑M1/1‑235 (MQ=255)                                                                                                ccCCAGCCGAGCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTCCCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTgg                                                                                                                                                                                      >  2:163465‑M1/3‑157 (MQ=255)                                                                                                                   gTAAGGCCCAGGCTTTCACCGACCATGTGCCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGc                                                                                                                                                   <  1:220530/173‑1 (MQ=255)                                                                                                                    tAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGAccacg                                                                                   <  1:640014/236‑1 (MQ=255)                                                                                                                               cTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCt                                                                                                                                                                                                                                      >  2:626348/1‑78 (MQ=255)                                                                                                                               cTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCt                                                                                                                                                                                                                                      <  1:626349/78‑1 (MQ=255)                                                                                                                                       tatcctcaacaacgggtacatggtcggtgaaagcctgggccttaccgagcacgatcggctggtggtgccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCa                                                                                              >  2:680452‑M2/70‑206 (MQ=255)                                                                                                                                           aTGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAg                                                                                                   >  1:567047/1‑197 (MQ=255)                                                                                                                                           aTGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCt                                                                                          >  2:434816/1‑206 (MQ=255)                                                                                                                                                       ttgAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGCCAACCTCGGCACGGGCCTGCAATGCGt                                                                                                                                  >  2:132527/1‑154 (MQ=255)                                                                                                                                                                 tGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGAccac                                                                                    >  2:747342/1‑190 (MQ=255)                                                                                                                                                                   gaaagcctgggccttaccgagcacgatcggctggtggtgccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGc                                                                                                                                    >  1:558711‑M2/42‑140 (MQ=255)                                                                                                                                                                   gaaagcctgggccttaccgagcacgatcggctggtggtgccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGc                                                                                                                                    <  2:558710‑M2/99‑1 (MQ=255)                                                                                                                                                                    tgtgGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGc                                                              >  2:320078/1‑209 (MQ=255)                                                                                                                                                                    tgtgGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGc                                                              <  1:320079/209‑1 (MQ=255)                                                                                                                                                                         cTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGc                              >  1:5058/1‑236 (MQ=255)                                                                                                                                                                                     cccTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGt                                                                                                           >  2:247087/1‑147 (MQ=255)                                                                                                                                                                                     cccTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGa                                                                                                      >  1:115227/1‑152 (MQ=255)                                                                                                                                                                                     cccTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCt                                                             >  2:547214/1‑193 (MQ=255)                                                                                                                                                                                           gggAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGAccacgcca                                                                                >  1:251498/1‑168 (MQ=255)                                                                                                                                                                                                   ggtggtgccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGGTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGGTGGCCAGGCCCGGGAGCAGGCCCt          >  1:240679‑M2/10‑230 (MQ=255)                                                                                                                                                                                                      ggtgccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCAGGCCCTGCAGCATg  <  1:747343‑M2/229‑1 (MQ=255)                                                                                                                                                                                                          ccTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCCGGCCCt          >  1:245219‑M2/3‑223 (MQ=255)                                                                                                                                                                                                            |                                                                                                                                                                                                                                     AAGGCATCGCTGGGGTAAATCAGTGCGCTGCCGTGTGTCATGCAGCCAAGGTTGGCCATGACCATGCCGAAGCAGTGGTACAGCGGCACCGGCACCACCAGCCGATCGTGCTCGGTAAGGCCCAGGCTTTCACCGACCATGTACCCGTTGTTGAGGATATTGCTGTGGCTAAGGGTGGCCCCCTTGGGGAAACCGGTGGTGCCTGAGGTGTACTGGATGTTGATCGGGTCGTCGCAACGCAGTTGTGCCTGGCGCGCGGCCAGGGCCTCGCCGCTGACAACCTCGGCACGGGCCTGCAATGCGTGCCAGGCCAGAAACCCCGGCGGTGGCGACAGCGCCAGGCTGACCACGCCACGCAGTTCGGGGAACCGCTCGCAGATCAGCGCACCCGGTTGGCTGCTGGCCAGGCCCGGGAGCAGGCCCTGCAGCATG  >  AE015451/3920933‑3921364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.