New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5956204	25 (0.630)	3 (0.080)	3/318	NT	10.6%	coding (263/489 nt)	PP_5221	conserved protein of unknown function, UPF0178 family
	?	AE015451	= 5956270	27 (0.710)					coding (197/489 nt)	PP_5221	conserved protein of unknown function, UPF0178 family

TTGGCAAACGCCTGTTTCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGG  >  AE015451/5956018‑5956433                                                                                                                                                                                           |                                                                                                                                                                                                                                      ttGGCAACCGCCTGTTTCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGc                                                                                                                                                                                                          <  2:468053/216‑1 (MQ=255)     cAAACGCCTGTTTCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAAAGTAgtc                                                                                                                                                                                   >  1:516940/1‑235 (MQ=255)          gCCTGTTTCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGt                                                                                                                                                                                                                                    <  2:499174/181‑1 (MQ=255)          gCCTGTTTCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGt                                                                                                                                                                                                                                    >  1:499175/1‑181 (MQ=255)            cTGTTTCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCCCAATGTAGTCGTCGGCCg                                                                                                                                                                           >  1:631927/1‑236 (MQ=255)             tGTTGCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGt                                                                                                                                                                                                                                    <  2:623733/178‑1 (MQ=255)                             cgtAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCt                                                                                                                                                                                                                  <  2:631898/178‑1 (MQ=255)                                aGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCAtggatgcggccgacgactacattgtggagcacgccgtgccgggcgagctggt                                                                                                                                                                                   <  2:143307‑M1/208‑53 (MQ=255)                                       cTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCGCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAgtcgtc                                                                                                                                                                                <  1:514688/204‑1 (MQ=255)                                                        ggCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATcgcg                                                                                                                                  >  2:673879/1‑233 (MQ=255)                                                                  aCGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAgt                                                                                                                                                                                    >  1:187735/1‑173 (MQ=255)                                                                    gCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAgtcgtc                                                                                                                                                                                <  2:510021/175‑1 (MQ=255)                                                                           ggTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACg                                                                                                                                                                                                       >  1:547997/1‑145 (MQ=255)                                                                           ggTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACg                                                                                                                                                                                                       <  2:547996/145‑1 (MQ=255)                                                                                 cAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACgg                                                                                                                                                                                                      <  1:741192/140‑1 (MQ=255)                                                                                 cAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACgg                                                                                                                                                                                                      >  2:741191/1‑140 (MQ=255)                                                                                          cgccgcCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGGGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATca                                                                                             >  1:423897/1‑236 (MQ=255)                                                                                          cgccgcCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCAtgc                                                                                                                                                                  <  2:512206‑M2/167‑4 (MQ=255)                                                                                            ccgccAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCATTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCAtgccgctggccgaccgcctggtgaaaaagggcg                                                                                                                                    >  1:465388‑M2/1‑162 (MQ=255)                                                                                                    cGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCCCCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGc                                                                                                                                                                             <  2:185469/146‑1 (MQ=255)                                                                                                                     cTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCg                                                                   <  1:673880/235‑1 (MQ=255)                                                                                                                           gAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTg                                                                                                                      >  1:737803/1‑178 (MQ=255)                                                                                                                           gAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTg                                                                                                                      <  2:737802/178‑1 (MQ=255)                                                                                                                           gAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGa                                                            >  1:552412/1‑236 (MQ=255)                                                                                                                             aCTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACg                                                          <  1:393076/236‑1 (MQ=255)                                                                                                                                                    gcgGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCGtggatgcggccggcgactacattgtggagcacgccgtgccgggcgagctgggg                                                                                                                                                                                  >  1:299270‑M1/1‑40 (MQ=255)                                                                                                                                                          aCGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGAGCAGGCGCACGATCGCGCACGCCGGCTTGGCCCCCGcc                                                                                                             >  2:84357/1‑156 (MQ=255)                                                                                                                                                           cGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCAtggatgcggccgacgactacattgtggagcacgccgtgccgggcgagctggtgatctgcagtgacgtgccgctggccgaccgcctggtgaaaatgggcgtggccgcgctggacccgcgtgggcgcgagttcgacgagcgcaacatgggcgaccgcctggcgg                                                                     >  1:465388‑M1/1‑33 (MQ=255)                                                                                                                                                           cGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAg                                                                                                                                               <  2:335643/121‑1 (MQ=255)                                                                                                                                                                tttttCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCgg                        >  1:450465/1‑235 (MQ=255)                                                                                                                                                                    tCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACGATCa                                                      <  1:748394/201‑1 (MQ=255)                                                                                                                                                                             ggTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATaca          >  1:367018/1‑236 (MQ=255)                                                                                                                                                                                    cAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCAtt    <  2:367017/235‑1 (MQ=255)                                                                                                                                                                                        ggCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGaa                                                                                   >  2:271099/1‑152 (MQ=255)                                                                                                                                                                                         gCAtggatgcggccgacgactacattgtggagcacgccgtgccgggcgagctggtgatctgcagtgacgtgccgctggccgaccgcctggtgaaaaagggcgtggccgcgctggacccgcgtgggcgcgagttcgacgagcgcaacatgggcgaccgcctggcggcg                                                                   <  2:512206‑M1/167‑165 (MQ=255)                                                                                                                                                                                         gCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTgg  <  2:552411/232‑1 (MQ=255)                                                                                                                                                                                           |                                                                                                                                                                                                                                      TTGGCAAACGCCTGTTTCTCACGTTCGCCATAGGCCGCCTGCCCGCCCCCCACCTGGCCCTGCTCACGCAGCTCGGTGAACAGGTTGCGCGCCGCCAGGCGGTCGCCCATGTTGCGCTCGTCGAACTCGCGCCCACGCGGGTCCAGCGCGGCCACGCCCTTTTTCACCAGGCGGTCGGCCAGCGGCACGTCACTGCAGATCACCAGCTCGCCCGGCACGGCGTGCTCCACAATGTAGTCGTCGGCCGCATCCATGCCGCTGGGCACCACGATCAGGCGCACGATCGCGAACGCCGGCTTGGCCACCGCCTGCCCCGCCACCATCACCACCTCGAACTTGCGCTTGAGGGCGAACTTGACGATCAGATCCTTGGCCGCCTTGGGGCAGGCGTCGGCATCGATCCATACACGCATTGG  >  AE015451/5956018‑5956433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.