New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	= 5433680	24 (0.600)	3 (0.080)	3/320	NT	10.2%	coding (1584/2529 nt)	hrpB	ATP‑dependent helicase HrpB
	?	AE015451	= 5433711	30 (0.790)					coding (1553/2529 nt)	hrpB	ATP‑dependent helicase HrpB

TCGGCCAGTACCCCCTCAAGCAACGCCGGATCGAATTCGGCTGCCAGATAGATCCGCTCCTCACGCTGCCCCTGGCGGCTACCCAGGTCGGCGATCACCAGCCATGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCAGCCACATCGCAGGCCATCTGTGC  >  AE015451/5433483‑5433943                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         tCGGCCAGTACCCCCTCAAGCAACGCCGGATCGAATTCGGCTGCCAGATAGATCCGCTCCTCACGCTGCCCCTGGCGGCTACCCAGGTCGGCGATCACCAGCCATGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGcgccca                                                                                                                                                                                                                                    >  1:50622‑M2/1‑229 (MQ=255)            ccccTCAAGCAACGCCGGATCGAATTCGGCTGCCAGATAGATCCGCTCCTCACGCTGCCCCTGGCGGCTACCCAGGTCGGCGATCACCAGCCATGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCgtgggt                                                                                                                                                                                                                        <  1:27077/236‑1 (MQ=255)             cccTCAAGCAACGCCGGATCGAATTCGGCTGCCAGATAGATCCGCTCCTCACGCTGCCCCTGGCGGCTACCCAGGTCGGCGATCACCAGCCATGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCccagcca                                                                                                                                                                                                                                >  2:639529/1‑227 (MQ=255)                                                                                   ccAGGTCGGCGACCACCAGCCATGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGa                                                                                                                                                 <  1:554040/236‑1 (MQ=255)                                                                                       gTCGGCGATCACCAGCCAGGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCAc                                                                                                                                             <  1:517221/236‑1 (MQ=255)                                                                                          ggCGATCACCAGCCATGGGCACTTCATCAATGCATCGACTGCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCCCCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGc                                                                                                                                               <  1:110512/231‑1 (MQ=255)                                                                                                       cctGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCa                                                                                                                                                         <  2:296778/206‑1 (MQ=255)                                                                                                                   atcaATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACg                                                                                                                                                                                   <  2:611086/170‑1 (MQ=255)                                                                                                                   atcaATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACg                                                                                                                                                                                   >  1:611087/1‑170 (MQ=255)                                                                                                                      aaTGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCAc                                                                                                                                             <  1:485502/205‑1 (MQ=255)                                                                                                                             cGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGct                                                                                                        >  1:393003/1‑235 (MQ=255)                                                                                                                                cTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGgccgc                                                                                                                        >  1:186028/1‑216 (MQ=255)                                                                                                                                  tCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCCGCGCCAGCAGGGCGCCCAGCCAGCGCGGCGGGTCCgg                                                                                                                                                                                                                 >  2:108008/1‑125 (MQ=255)                                                                                                                                  tCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCAccc                                                                                                                               >  1:23053/1‑207 (MQ=255)                                                                                                                                    gCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCa                                                                                                    <  1:137292/232‑1 (MQ=255)                                                                                                                                                cGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGa                                                                                                                                                 <  1:520379/175‑1 (MQ=255)                                                                                                                                                cGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGc                                                                                                         >  1:262541/1‑215 (MQ=255)                                                                                                                                                cGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGc                                                                                                         <  2:262540/215‑1 (MQ=255)                                                                                                                                                     ggCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGGGGTCCGGGTCGGCGACCGGCGCACCGAc                                                                                                                                                                                            >  2:253499/1‑127 (MQ=255)                                                                                                                                                             gCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCaa                                                                       <  1:639530/236‑1 (MQ=255)                                                                                                                                                                        cTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGc                                                                                  <  1:501991/214‑1 (MQ=255)                                                                                                                                                                                                       aCCCGGTCAGGGTAGGCCAGGGCCAGAAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGAACCGACCTGGCCACGCAACa                                                                                                                                                                              <  2:45754/91‑1 (MQ=255)                                                                                                                                                                                                       aCCCGGTCAGGGTAGGCCAGCGCCAGCAGGGcgcccagcagcgccgggcgggtggggcggagtatcggctggccaacggcc                                                                                                                                                                                        >  1:486814‑M2/1‑31 (MQ=255)                                                                                                                                                                                                       aCCCGGTCAGGGTAGGCCAGCGCCAGCAGGGcgcccagcagcgccgggcgggtggggcggagtatcggctggccaacggcc                                                                                                                                                                                        <  2:486813‑M2/81‑51 (MQ=255)                                                                                                                                                                                                           ggTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCaa                                                                       >  2:138402/1‑190 (MQ=255)                                                                                                                                                                                                           ggTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTg                                                  >  1:407670/1‑211 (MQ=255)                                                                                                                                                                                                           ggTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCa                         >  1:690560/1‑236 (MQ=255)                                                                                                                                                                                                               aGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCAg                        <  1:602591/233‑1 (MQ=255)                                                                                                                                                                                                                gggTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGTCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCAGCca                     <  1:398225/235‑1 (MQ=255)                                                                                                                                                                                                                      gccagcgccagcAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCAGCCACATCGc               <  1:436456/235‑1 (MQ=255)                                                                                                                                                                                                                              cagcagGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGa                                                                                                                                                    >  2:647601/1‑94 (MQ=255)                                                                                                                                                                                                                              cagcagGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGcc                                                                                                                                          >  2:224861/1‑103 (MQ=255)                                                                                                                                                                                                                              cagcagGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCtgt                                                                           >  2:377669/1‑167 (MQ=255)                                                                                                                                                                                                                              cagcagGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCAGCCACATCGCAGGCCAt        >  2:381829/1‑234 (MQ=255)                                                                                                                                                                                                                               agcggGGcgccgggcagcgccgggcgggtggggcggagtatcggctggccaacggccgtgcggccctgtttggcgaggtcgatgcattgatgaagtgcccatggctggtgatcgccgacctgggtagccgccaggggcagcgtga                                                                                                >  2:75821‑M2/1‑7 (MQ=255)                                                                                                                                                                                                                                   gggCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCAGCCACATCGCAGGCCATCTGTGc  >  1:479904/1‑235 (MQ=255)                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                         TCGGCCAGTACCCCCTCAAGCAACGCCGGATCGAATTCGGCTGCCAGATAGATCCGCTCCTCACGCTGCCCCTGGCGGCTACCCAGGTCGGCGATCACCAGCCATGGGCACTTCATCAATGCATCGACTTCGCCAAACAGGGCCGCACGGCCGTTGGCCAGCCGATACTCCGCCCCACCCGCCCGGCGCTGCTGGGCGACCCGGTCAGGGTAGGCCAGCGCCAGCAGGGCGCCCAGCCAGCGTGGGTGGTCCGGGTCGGCGACCGGCGCACCGACCTTGCCACGCAACAGGCCACGGTATTGCCGGGCCAGCTGACGAGCACGCTGCACACCACCCTGGCCGCCACGCGCTGCCTTGCTCTCACCGCTGACCAGCGCAAGCCGGCTGTGCAAGTCGGCACCACCGCCACGCTGGATGTCCCGCTCCCCCAGCAGTGCAGCCACATCGCAGGCCATCTGTGC  >  AE015451/5433483‑5433943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.