New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AE015451	1217706 =	75 (1.210)	6 (0.100)	4/324	NT	8.5%	coding (704/918 nt)	metR‑I	DNA‑binding transcriptional regulator, homocysteine‑binding
	?	AE015451	1217888 =	59 (1.010)					coding (522/918 nt)	metR‑I	DNA‑binding transcriptional regulator, homocysteine‑binding

GGCATCACTTACGTGCCGCTGTTCACCTACGAGGCGATGCTGGCAGTGGCCAACCAACACCCGCTGGCCAGCAAGCCTTACATCGTGCCGCAAGACTTGCTCGACCAGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGCCAGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/1217947‑1217706 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggccagcggGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATTCAATAGC  >  AE015451/1217888‑1218121                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              GGCATCACTTACGTGCCGCTGTTCACCTACGAGGCGATGCTGGCAGTGGCCAACCAACACCCGCTGGCCAGCAAGCCTTACATCGTGCCGCAAGACTTGCTCGACCAGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGC                                                                                                                                                                                                                                                  <  1:903678/236‑1                                           GCAGTGGCCAACCAACACCCGCTGGCCAGCAAGCCTTACATCGTGCCGCAAGACTTGCTCGACCAGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGCCAGCGGGTGTTGGTTG                                                                                                                                                                                                                                  <  2:448142/210‑1                                                                  GGCCAGCAAGCCTTACATCGTGCCGCAAGCCTTGCTCGACCAGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGT                                                                                                                                                                                                                                                     <  2:894463/168‑1                                                                                         CGCAAGACTTGCTCGACCAGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGCGCTGACGGGGATGATGATGCAACTGGTGGCCAG                                                                                                                                                                                                                                               >  2:226491/1‑151                                                                                               ACTTGCTCGACCAGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCC                                                                                                                                                                                                                <  1:771337/176‑1                                                                                                           AGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAA                                                                                                                                                                                                     <  1:680336/175‑1                                                                                                                                TGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGC                                                                                                                                                                                                                                                  >  1:731117/1‑109                                                                                                                                TGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGC                                                                                                                                                                                                                                                  <  2:731116/109‑1                                                                                                                                       CGATCGCCCGGACATCTTCACCCGCCTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCC                                                                                                                                                                                                                <  2:466096/136‑1                                                                                                                                                                   CTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAG                                                                                >  1:328381/1‑236                                                                                                                                                                                                                               agGCAACTGGTGGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAA                                                                                                                                                                                                     <  1:198535/57‑1                                                                                                                                                                                                                                         TGGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTG              >  2:407325/1‑232                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGTCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGTAGCAACTGTGGCATT         >  1:276545/1‑236                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGTCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTC                                                                                                                                                                       <  2:1063544/78‑1                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTCGCGGGGCGCAGGCCAAGCC                                                                                               >  2:145587/1‑150                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGCAGGCAAAGCCCGAGGCCAGG                                                                                     >  1:210911/1‑160                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGg                                                >  1:101761/1‑196                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCAct         >  1:830037/1‑234                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATT         >  1:1022788/1‑236                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATT         >  1:1079214/1‑236                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATT         >  1:747219/1‑236                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCAT          >  1:196565/1‑235                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCAT          >  1:261200/1‑235                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAG                                >  2:552746/1‑213                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGGCCAGTTCCACTTCCGGC                                                                   >  1:345828/1‑178                                                                                                                                                                                                                                          GGCCAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGAAGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGa                                                                   >  1:94924/1‑177                                                                                                                                                                                                                                          GGCCAGCGGGTATTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTC                                                                                                                                                                       >  1:1063545/1‑78                                                                                                                                                                                                                                             CAGCGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATTCAA      <  1:407326/236‑1                                                                                                                                                                                                                                                CGGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATTCAA      <  2:1076058/233‑1                                                                                                                                                                                                                                                CGGGTGTCGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGAAACTGGTCGATGGTCGGCTTC                                  <  1:622935/205‑1                                                                                                                                                                                                                                                 GGGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATTCAATAGC  >  1:1106562/1‑236                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              GGCATCACTTACGTGCCGCTGTTCACCTACGAGGCGATGCTGGCAGTGGCCAACCAACACCCGCTGGCCAGCAAGCCTTACATCGTGCCGCAAGACTTGCTCGACCAGACGCTGATCACCTACCCGGTGGAGCGCGATCGCCTGGACATCTTCACGCGCTTCCTGGAGCCGGCCGATATCGAGCCAGCCGCCGTGCGTACCTCGGAGCTGACGGTGATGATGATGCAACTGGTGGCCAGCGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  AE015451/1217947‑1217706 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑tggccagcggGTGTTGGTTGGCCACTGCCAGCATCGCCTCGTAGGTGAACAGCGGCACGTAAGTGATGCCTGCCAGGTCCACCGGGTCGGAGGTCACCACCAGGTCAAGGTCGCCGCGGGCCAGGGCCGGTAGCGGGGCGAAGGCAAAGCCCGAGGCCAGGTCCAGTTCCACTTCCGGCCAGGCATCACGGAACTGGTCGATGGTCGGCATCAGCCACTGGAAGCAACTGTGGCATTCAATAGC  >  AE015451/1217888‑1218121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.