Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	CP009273	4,303,167	Δ450 bp	100%	coding (1‑450/450 nt)	rpiB →	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4303167	4303616	450	54 [0]	[0] 54	rpiB	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4303166	0 (0.000)	53 (0.880)	47/270	NT	100%	intergenic (‑358/‑1)	alsR/rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
	?	CP009273	4303617 =	0 (0.000)					intergenic (+1/‑68)	rpiB/yjdP	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase/putative periplasmic protein

AACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGAC  >  CP009273/4303473‑4303762                                                                                                                                                 |                                                                                                                                                  agtgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCa                                                                                                                                              >  1:496639‑M2/145‑150 (MQ=255) agtgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggagtttttaattcattaacatcacacatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCa                                                                                                                                              >  2:505400‑M2/145‑150 (MQ=255)   tgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATc                                                                                                                                            <  1:1029724‑M2/8‑1 (MQ=255)         taacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCact                                                                                                                                        <  2:385082‑M2/12‑1 (MQ=255)                 ttgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCAt                                                                                                                                <  1:605321‑M2/20‑1 (MQ=255)                 ttgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATg                                                                                                                               <  1:717108‑M2/21‑1 (MQ=255)                    cgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATg                                                                                                                           <  1:866398‑M2/25‑1 (MQ=255)                        gtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGt                                                                                                                        >  2:850465‑M2/122‑149 (MQ=255)                             atgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGAt                                                                                                                  >  1:123348‑M2/117‑150 (MQ=255)                                taactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTa                                                                                                                <  2:907189‑M2/36‑1 (MQ=255)                                  actctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAAt                                                                                                              <  2:473606‑M2/38‑1 (MQ=255)                                    tctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAAtgtg                                                                                                           >  1:845571‑M2/110‑150 (MQ=255)                                             cagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGtt                                                                                                  >  1:694481‑M2/101‑150 (MQ=255)                                                   aggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCtttt                                                                                            <  1:631957‑M2/56‑1 (MQ=255)                                                    ggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCttttt                                                                                           <  2:373622‑M2/57‑1 (MQ=255)                                                       aggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAg                                                                                        <  1:895592‑M2/60‑1 (MQ=255)                                                             aattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGc                                                                                  >  2:668262‑M2/85‑150 (MQ=255)                                                                      attttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAAc                                                                         <  2:651826‑M2/75‑1 (MQ=255)                                                                       ttttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACg                                                                        <  1:396982‑M2/76‑1 (MQ=255)                                                                            ggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTc                                                                   >  2:7305‑M2/70‑150 (MQ=255)                                                                            ggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTc                                                                   >  2:1062062‑M2/70‑150 (MQ=255)                                                                             gtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTcc                                                                  <  2:166561‑M2/82‑1 (MQ=255)                                                                              tgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCg                                                                 >  2:448900‑M2/68‑150 (MQ=255)                                                                              tgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCg                                                                 >  1:776114‑M2/68‑150 (MQ=255)                                                                                tttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGc                                                                >  1:312174‑M2/66‑149 (MQ=255)                                                                                          attaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                     >  1:51877‑M2/56‑150 (MQ=255)                                                                                              acatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCa                                                 >  2:333821‑M2/52‑150 (MQ=255)                                                                                                 tcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGCAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAccc                                              >  1:279233‑M2/49‑150 (MQ=255)                                                                                                   acaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCt                                             <  2:155841‑M2/103‑1 (MQ=255)                                                                                                   acaaatgttttttgattgcgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGCGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTg                                            >  2:92523‑M2/47‑150 (MQ=255)                                                                                                     aaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                          <  1:507264‑M2/106‑1 (MQ=255)                                                                                                          ttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACg                                      <  1:145026‑M2/110‑1 (MQ=255)                                                                                                          ttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACg                                      <  2:123348‑M2/110‑1 (MQ=255)                                                                                                          ttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGc                                     >  1:743652‑M2/40‑150 (MQ=255)                                                                                                          ttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGc                                     >  1:702931‑M2/40‑150 (MQ=255)                                                                                                           tttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCt                                    >  2:594634‑M2/39‑150 (MQ=255)                                                                                                            ttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCt                                    <  1:668262‑M2/112‑1 (MQ=255)                                                                                                                gattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCa                               >  1:568352‑M2/34‑150 (MQ=255)                                                                                                                gattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCa                               >  2:276018‑M2/34‑150 (MQ=255)                                                                                                                 attgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAc                              >  1:995593‑M2/33‑150 (MQ=255)                                                                                                                  ttgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTg                                            >  2:8876‑M2/32‑135 (MQ=255)                                                                                                                   tgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGc                                           <  1:8876‑M2/105‑1 (MQ=255)                                                                                                                       aagttttgcgcggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTc                         <  1:1062062‑M2/123‑1 (MQ=255)                                                                                                                       aagttttgcaaggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTcc                        <  1:493115‑M2/124‑1 (MQ=255)                                                                                                                        agttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCg                       >  2:469207‑M2/26‑150 (MQ=255)                                                                                                                         gttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCgg                      >  1:675164‑M2/25‑150 (MQ=255)                                                                                                                               cacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAg                 <  1:59662‑M2/131‑1 (MQ=255)                                                                                                                                 cggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGcc              >  1:66810‑M2/17‑150 (MQ=255)                                                                                                                                  ggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGcc              >  1:544542‑M2/16‑149 (MQ=255)                                                                                                                                   gacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGa            >  2:166999‑M2/15‑150 (MQ=255)                                                                                                                                    acggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGa            >  2:196614‑M2/14‑149 (MQ=255)                                                                                                                                     cggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGata          >  1:1025688‑M2/13‑150 (MQ=255)                                                                                                                                        ggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGAtatta       >  1:365889‑M2/10‑150 (MQ=255)                                                                                                                                             atgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac  <  1:198465‑M2/146‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  AACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGAC  >  CP009273/4303473‑4303762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑