Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
JC JC	CP009273	2,770,391	IS2 (+) +5 bp	20.0%	coding (156‑160/201 nt)	ypjJ →	uncharacterized protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	2770391 =	38 (0.660)	8 (0.130)	8/276	NT	16.9%	coding (156/201 nt)	ypjJ	uncharacterized protein
	?	CP009273	2989720 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	CP009273	= 2770395	38 (0.660)	11 (0.180)	11/276	NT	21.8%	coding (160/201 nt)	ypjJ	uncharacterized protein
	?	CP009273	= 2991050	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

CTCCCAGGCGCGGCGTGATATCTCGCTGCAGGCCCCATGTGGTGTTGCTCATTCATAACCTCTTTATTGTCAGTTCAGGGTGATACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2770525‑2770391 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTAT  >  CP009273/2989720‑2989837                                                                                                                                                                                                                                                               CTCCCAGGCGCGGCGTGATATCTCGCTGCAGGCCCCATGTGGTGTTGCTCATTCATAACCTCTTTATTGTCAGTTCAGGGTGATACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTA                                                                                                          >  2:457938/1‑149                            GCAGGCCCCATGTGGTGTTGCTCATTCATAACCTCTTTATTGTCAGTTCAGGGTGATACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAAC                                                                              >  1:779956/1‑150                                GCCCCATGTGGTGTTGCTCATTCATAACCTCTTTATTGTCAGTTCAGGGTGATACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCAT                                                                          >  1:943228/1‑150                                   CCATGTGGTGTTGCTCATTCATAACCTCTTTATTGTCAGTTCAGGGTGATACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTA                                                                        >  2:203921/1‑149                                                                            CAGGGTGATACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACC                               <  2:590285/149‑1                                                                                    TACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCAC                       >  1:941462/1‑149                                                                                          TCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCG                 >  1:451749/1‑149                                                                                                        GGTCCGCTGCGGTCCTGCGGCGTTCGGCGTACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTAT  <  2:451749/150‑1                                                                                                                                                                                                                                                               CTCCCAGGCGCGGCGTGATATCTCGCTGCAGGCCCCATGTGGTGTTGCTCATTCATAACCTCTTTATTGTCAGTTCAGGGTGATACTCATCAGTCAAGCATACGGTCCGCTGCGGTCCTGCGGCGTTCGGCGTAC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  CP009273/2770525‑2770391 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTAT  >  CP009273/2989720‑2989837

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.