Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	CP009273	4,303,167	Δ450 bp	100%	coding (1‑450/450 nt)	rpiB →	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4303167	4303616	450	67 [2]	[1] 66	rpiB	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4303166	2 (0.030)	61 (1.040)	54/270	NT	97.6%	intergenic (‑358/‑1)	alsR/rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
	?	CP009273	4303617 =	1 (0.020)					intergenic (+1/‑68)	rpiB/yjdP	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase/putative periplasmic protein

TGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCA  >  CP009273/4303021‑4303312                                                                                                                                                  |                                                                                                                                                   tGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgatt                                                                                                                                                <  1:432234‑M1/150‑5 (MQ=255)     tgtCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatc                                                                                                                                            <  1:569658‑M1/150‑9 (MQ=255)       tCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatcca                                                                                                                                          <  1:141680‑M1/150‑11 (MQ=255)            aaCGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactact                                                                                                                                     >  2:54835‑M1/1‑135 (MQ=255)             aCGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGTCGGGGAAGATGAgattcatccactact                                                                                                                                     <  1:631253‑M1/149‑16 (MQ=255)             aCGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactactt                                                                                                                                    >  2:200152‑M1/1‑134 (MQ=255)                cTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgca                                                                                                                                 <  1:706107‑M1/150‑20 (MQ=255)                          gTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagt                                                                                                                        >  1:576658‑M1/1‑121 (MQ=255)                             aGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaat                                                                                                                     >  1:591436‑M1/1‑118 (MQ=255)                               aTGTAACTCTGTAAAACAGATCAGGAAGGAGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgat                                                                                                                  <  1:157026‑M1/150‑35 (MQ=255)                                 gTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgatt                                                                                                                 >  1:274970‑M1/1‑114 (MQ=255)                                  tAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaa                                                                                                               >  1:833217‑M1/1‑113 (MQ=255)                                          tAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggat                                                                                                        <  1:837271‑M1/149‑45 (MQ=255)                                          tAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtgg                                                                                                          <  1:778336‑M1/147‑43 (MQ=255)                                            aaaCAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatag                                                                                                      <  2:215486‑M1/149‑47 (MQ=255)                                            aaaCAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggata                                                                                                       >  2:239803‑M1/1‑103 (MQ=255)                                            aaaCAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggata                                                                                                       >  2:576968‑M1/1‑103 (MQ=255)                                             aaCAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagag                                                                                                    <  1:228721‑M1/150‑49 (MQ=255)                                              aCAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagt                                                                                                   >  2:877489‑M1/1‑101 (MQ=255)                                              aCAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagt                                                                                                   >  1:273688‑M1/1‑101 (MQ=255)                                                aGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagttt                                                                                                 <  1:178111‑M1/150‑52 (MQ=255)                                                 gATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttc                                                                                                >  1:670582‑M1/1‑98 (MQ=255)                                                     aGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttt                                                                                            >  1:18990‑M1/1‑94 (MQ=255)                                                          ggCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgagg                                                                                       >  2:13746‑M1/1‑89 (MQ=255)                                                             gTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttg                                                                                    >  1:519846‑M1/1‑86 (MQ=255)                                                                    aTCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGt                                                                             >  1:886068/1‑150 (MQ=255)                                                                     tCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatga                                                                            <  1:876331‑M1/150‑73 (MQ=255)                                                                      cGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatga                                                                            >  1:202807‑M1/1‑77 (MQ=255)                                                                       gATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaa                                                                          >  2:237051‑M1/1‑76 (MQ=255)                                                                        aTTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaac                                                                         >  2:453574‑M1/1‑75 (MQ=255)                                                                           ttGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgct                                                                      >  1:495968‑M1/1‑72 (MQ=255)                                                                              ggTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttc                                                                   <  2:125456‑M1/150‑82 (MQ=255)                                                                               gtgtTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactgcttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttcc                                                                  <  2:541703‑M1/150‑83 (MQ=255)                                                                               gtgtTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttcc                                                                  <  2:876939‑M1/150‑83 (MQ=255)                                                                                    tttAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatgggtgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctat                                                             >  1:163647‑M1/1‑63 (MQ=255)                                                                                     ttAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctat                                                             <  1:272551‑M1/149‑88 (MQ=255)                                                                                     ttAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctat                                                             <  1:492329‑M1/149‑88 (MQ=255)                                                                                         ttCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttctt                                                        >  2:613145‑M1/1‑58 (MQ=255)                                                                                           cATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttt                                                       <  2:591436‑M1/149‑94 (MQ=255)                                                                                            aTTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttctttta                                                     >  2:613093‑M1/1‑55 (MQ=255)                                                                                            aTTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttctttta                                                     <  2:18990‑M1/150‑96 (MQ=255)                                                                                            aTTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttctttt                                                      <  2:621445‑M1/149‑95 (MQ=255)                                                                                             ttAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttat                                                    <  1:203281‑M1/150‑97 (MQ=255)                                                                                             ttAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttat                                                    <  2:274970‑M1/150‑97 (MQ=255)                                                                                              tAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttatt                                                   >  1:152285‑M1/1‑53 (MQ=255)                                                                                              tAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttatt                                                   >  2:376750‑M1/1‑53 (MQ=255)                                                                                               aaCATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttatt                                                   >  1:520650‑M1/1‑52 (MQ=255)                                                                                                aCATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattc                                                  <  1:395348‑M1/149‑99 (MQ=255)                                                                                                   tCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccc                                              <  2:576658‑M1/150‑103 (MQ=255)                                                                                                       aaaTGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgct                                          >  1:164018‑M1/1‑44 (MQ=255)                                                                                                        aaTGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctc                                         <  2:853962‑M1/150‑108 (MQ=255)                                                                                                        aaTGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgct                                          >  1:528367‑M1/1‑43 (MQ=255)                                                                                                          tGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcac                                       >  1:313414‑M1/1‑41 (MQ=255)                                                                                                                   aTTGTGAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccac                              >  2:736668‑M1/1‑32 (MQ=255)                                                                                                                    ttGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCg                             >  1:1016994/1‑150 (MQ=255)                                                                                                                     tgtgAAGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccg                            >  2:136323‑M1/1‑30 (MQ=255)                                                                                                                         aaGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttcc                        <  1:877489‑M1/150‑125 (MQ=255)                                                                                                                          aGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccg                       >  1:231059‑M1/1‑25 (MQ=255)                                                                                                                          aGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccg                       <  2:852617‑M1/150‑126 (MQ=255)                                                                                                                          aGTTTTGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccg                       >  2:111969‑M1/1‑25 (MQ=255)                                                                                                                               tGCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcc                    >  2:903932‑M1/1‑20 (MQ=255)                                                                                                                                gCACGGACGGGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggccca                  <  2:833217‑M1/149‑131 (MQ=255)                                                                                                                                    ggacggGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggcc              >  1:186552‑M1/1‑15 (MQ=255)                                                                                                                                     gacggGGAAGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccga            >  2:570530‑M1/1‑14 (MQ=255)                                                                                                                                             aGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgatattatcg    <  1:54835‑M1/150‑145 (MQ=255)                                                                                                                                             aGATGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgatattatcg    <  1:239803‑M1/150‑145 (MQ=255)                                                                                                                                               aTGAgattcatccactacttgcatggatgagtaatgattaatgtggatagagtttctttttgaggttggctaatgaaacgctttccgctatttcttttattcaccctgctcacgctgtccaccgttccggcccaggccgatattatcgac  <  1:268437‑M1/150‑147 (MQ=255)                                                                                                                                                  |                                                                                                                                                   TGAGTGTCTTTAACGCTATTGCGCTGTCAGATGTAACTCTGTAAAACAGATCAGGAAGGCGTAATTCATCGATTTTGGGTGTTTTTAATTCATTAACATCACAAATGTTTTTTGATTGTGAAGTTTTGCACGGACGGGGAAGATGAATGAAAAAGATTGCATTTGGCTGTGATCATGTCGGTTTCATTTTAAAACATGAAATAGTGGCACATTTAGTTGAGCGTGGCGTTGAAGTGATTGATAAAGGAACCTGGTCGTCAGAGCGTACTGATTATCCACATTACGCCAGTCA  >  CP009273/4303021‑4303312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑