Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
MC JC	CP009273	4,303,167	Δ450 bp	100%	coding (1‑450/450 nt)	rpiB →	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	CP009273	4303167	4303616	450	67 [2]	[1] 66	rpiB	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP009273	= 4303166	2 (0.030)	61 (1.040)	54/270	NT	97.6%	intergenic (‑358/‑1)	alsR/rpiB	d‑allose‑inducible als operon transcriptional repressor; autorepressor; repressor of rpiR/ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase
	?	CP009273	4303617 =	1 (0.020)					intergenic (+1/‑68)	rpiB/yjdP	ribose 5‑phosphate isomerase B/allose 6‑phosphate isomerase/putative periplasmic protein

CCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGAC  >  CP009273/4303471‑4303762                                                                                                                                                   |                                                                                                                                                  tgagtgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGAtt                                                                                                                                                <  1:432234‑M2/4‑1 (MQ=255)     tgtctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATc                                                                                                                                            <  1:569658‑M2/8‑1 (MQ=255)       tctttaacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCa                                                                                                                                          <  1:141680‑M2/10‑1 (MQ=255)            aacgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCactact                                                                                                                                     >  2:54835‑M2/136‑150 (MQ=255)             acgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggtcggggaagatgaGATTCATCCactact                                                                                                                                     <  1:631253‑M2/15‑1 (MQ=255)             acgctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACtt                                                                                                                                    >  2:200152‑M2/135‑150 (MQ=255)                ctattgcgctgtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCa                                                                                                                                 <  1:706107‑M2/19‑1 (MQ=255)                          gtcagatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGt                                                                                                                        >  1:576658‑M2/122‑149 (MQ=255)                             agatgtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAAt                                                                                                                     >  1:591436‑M2/119‑149 (MQ=255)                               atgtaactctgtaaaacagatcaggaaggagtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGAt                                                                                                                  <  1:157026‑M2/34‑1 (MQ=255)                                 gtaactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGAtt                                                                                                                 >  1:274970‑M2/115‑149 (MQ=255)                                  taactctgtaaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTaa                                                                                                               >  1:833217‑M2/114‑150 (MQ=255)                                          taaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTgg                                                                                                          <  1:778336‑M2/42‑1 (MQ=255)                                          taaaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGAt                                                                                                        <  1:837271‑M2/44‑1 (MQ=255)                                            aaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATag                                                                                                      <  2:215486‑M2/46‑1 (MQ=255)                                            aaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATa                                                                                                       >  2:239803‑M2/104‑148 (MQ=255)                                            aaacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATa                                                                                                       >  2:576968‑M2/104‑148 (MQ=255)                                             aacagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATagag                                                                                                    <  1:228721‑M2/48‑1 (MQ=255)                                              acagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGt                                                                                                   >  1:273688‑M2/102‑150 (MQ=255)                                              acagatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGt                                                                                                   >  2:877489‑M2/102‑150 (MQ=255)                                                agatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGttt                                                                                                 <  1:178111‑M2/51‑1 (MQ=255)                                                 gatcaggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTc                                                                                                >  1:670582‑M2/99‑150 (MQ=255)                                                     aggaaggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCtttt                                                                                            >  1:18990‑M2/95‑150 (MQ=255)                                                          ggcgtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAgg                                                                                       >  2:13746‑M2/90‑150 (MQ=255)                                                             gtaattcatcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTg                                                                                    >  1:519846‑M2/87‑150 (MQ=255)                                                             aTGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTg                                                                                    <  2:708559/150‑1 (MQ=255)                                                                     tcgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGa                                                                            <  1:876331‑M2/72‑1 (MQ=255)                                                                      cgattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGa                                                                            >  1:202807‑M2/78‑149 (MQ=255)                                                                       gattttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGaaa                                                                          >  2:237051‑M2/77‑150 (MQ=255)                                                                        attttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAAc                                                                         >  2:453574‑M2/76‑150 (MQ=255)                                                                           ttgggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCt                                                                      >  1:495968‑M2/73‑150 (MQ=255)                                                                              ggtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTc                                                                   <  2:125456‑M2/81‑1 (MQ=255)                                                                               gtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTGCTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTcc                                                                  <  2:541703‑M2/82‑1 (MQ=255)                                                                               gtgtttttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTcc                                                                  <  2:876939‑M2/82‑1 (MQ=255)                                                                                    tttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGGTGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                             >  1:163647‑M2/64‑150 (MQ=255)                                                                                     ttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                             <  1:272551‑M2/87‑1 (MQ=255)                                                                                     ttaattcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTAt                                                             <  1:492329‑M2/87‑1 (MQ=255)                                                                                         ttcattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCtt                                                        >  2:613145‑M2/59‑150 (MQ=255)                                                                                           cattaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCttt                                                       <  2:591436‑M2/93‑1 (MQ=255)                                                                                            attaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCtttt                                                      <  2:621445‑M2/94‑1 (MQ=255)                                                                                            attaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                     <  2:18990‑M2/95‑1 (MQ=255)                                                                                            attaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTa                                                     >  2:613093‑M2/56‑150 (MQ=255)                                                                                             ttaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAt                                                    <  2:274970‑M2/96‑1 (MQ=255)                                                                                             ttaacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAt                                                    <  1:203281‑M2/96‑1 (MQ=255)                                                                                              taacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                                   >  2:376750‑M2/54‑150 (MQ=255)                                                                                              taacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                                   >  1:152285‑M2/54‑150 (MQ=255)                                                                                               aacatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTAtt                                                   >  1:520650‑M2/53‑149 (MQ=255)                                                                                                acatcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTc                                                  <  1:395348‑M2/98‑1 (MQ=255)                                                                                                   tcacaaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCAccc                                              <  2:576658‑M2/102‑1 (MQ=255)                                                                                                       aaatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                          >  1:164018‑M2/45‑150 (MQ=255)                                                                                                        aatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCt                                          >  1:528367‑M2/44‑149 (MQ=255)                                                                                                        aatgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTc                                         <  2:853962‑M2/107‑1 (MQ=255)                                                                                                          tgttttttgattgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCAc                                       >  1:313414‑M2/42‑150 (MQ=255)                                                                                                                   attgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCAc                              >  2:736668‑M2/33‑150 (MQ=255)                                                                                                                     tgtgaagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCg                            >  2:136323‑M2/31‑150 (MQ=255)                                                                                                                         aagttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTcc                        <  1:877489‑M2/124‑1 (MQ=255)                                                                                                                          agttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCg                       >  2:111969‑M2/26‑150 (MQ=255)                                                                                                                          agttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCg                       <  2:852617‑M2/125‑1 (MQ=255)                                                                                                                          agttttgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCg                       >  1:231059‑M2/26‑150 (MQ=255)                                                                                                                               tgcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGcc                    >  2:903932‑M2/21‑148 (MQ=255)                                                                                                                                gcacggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCa                  <  2:833217‑M2/130‑1 (MQ=255)                                                                                                                                    ggacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGcc              >  1:186552‑M2/16‑149 (MQ=255)                                                                                                                                     gacggggaagatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGa            >  2:570530‑M2/15‑150 (MQ=255)                                                                                                                                             agatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcg    <  1:239803‑M2/144‑1 (MQ=255)                                                                                                                                             agatgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcg    <  1:54835‑M2/144‑1 (MQ=255)                                                                                                                                               atgaGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATcgac  <  1:268437‑M2/146‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  CCAACGTGCTGGCTTTTGGTTCACGAGTGGTTGGCCTCGAACTGGCAAAAATGATTGTGGATGCGTGGCTGGGCGCACAGTACGAAGGCGGTCGTCATCAACAACGCGTGGAGGCGATTACGGCAATAGAGCAGCGGAGAAATTGAGATTCATCCACTACTTGCATGGATGAGTAATGATTAATGTGGATAGAGTTTCTTTTTGAGGTTGGCTAATGAAACGCTTTCCGCTATTTCTTTTATTCACCCTGCTCACGCTGTCCACCGTTCCGGCCCAGGCCGATATTATCGAC  >  CP009273/4303471‑4303762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑