New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 2653669231 (1.290)5 (0.030)
+CGGCAT
4/228 NT 2.1% coding (598/1161 nt) safA spore coat assembly protein SafA
?NZ_CP034943 2653670 = 240 (1.280)coding (597/1161 nt) safA spore coat assembly protein SafA
Rejected: Frequency below/above cutoff threshold.

GTATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2653532‑2653669
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCATATTCGGAACTGTGTGATGAACGTTTTCTTCTTCCATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATATTCGGATAATTTGCATTTTCCATATTACTCATAGCCTCCTG  >  NZ_CP034943/2653670‑2653789
                                                                                                                                          ||||||                                                                                                                        
GTATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATTAGCCGGATAATGATGATAAGGCTGAACAGCCG                                                                                                                              >  1:758753/1‑140
GTATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCG                                                                                                                              >  1:1024445/1‑140
GTATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCG                                                                                                                              >  1:1796761/1‑140
GTATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCG                                                                                                                              >  1:2615462/1‑140
  ATCCATGTGGCTGATAGATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  2:2453491/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:1255511/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  2:471850/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  2:1340743/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  2:1227228/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  2:1175804/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  2:1167651/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:543472/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:543217/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:396719/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:377721/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:3005672/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:2318283/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:2274285/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:2132587/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:2016925/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:1961388/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:1765702/140‑1
  ATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:1338003/140‑1
  ATCCATGGGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGCCGGC                                                                                                                            <  1:2132581/140‑1
                         TAAGCTTGTGCCGGATAGTACGGATAGCATAATCCTGATCCTGGAAGAATTGGCGAAACAGGCACCGGACACGGTACGAAATGAGCCGGATAATGATAATAAGGCTGAACAGCCGGCATCGGCATATTCGGAACTGTGTt                                                                                                     >  2:2286272/1‑139
                                           TACGGATAGCATAATCCTGATCCTGGAAGAATTGGCGAAACAGGCACCGGACACGGTACGAAATGAGCCGGATAATGATAATAAGGCTGAACAGCCGGCATCGGCATATTCGGAACTGTGTGATGAACGTTTTCCTCTTC                                                                                   >  1:311690/1‑140
                                                                                       cACCGGACACGGTACGAAATGAGCCGGATAATGATAATAAGGCTGAACAGCCGGCATCGGCATATTCGGAACTGTGTGATGAACGTTTTCCTCTTCTATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATAT                                          >  1:2996533/2‑137
                                                                                       cACCGGACACGGTACGAAATGAGCCGGATAATGATAATAAGGCTGAACAGCCGGCATCGGCATATTCGGAACTGTGTGATGAACGTTTTCCTCTTCTATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATAT                                          <  2:2996533/136‑1
                                                                                                                            TAAGGCTGAACAGCCGGCATCGGCATATTCGGAACTGTGTGATGAACGTTTTCCTCTTCTATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATATTCGGATAATTTGCATTTTCCATATTACTCATAGCCTCCTG  <  2:311690/140‑1
                                                                                                                                          ||||||                                                                                                                        
GTATCCATGTGGCTGATACATTGGATAAGCTTGTGCCGGATAGTACGGGTAGCATAATCCTGACCCTGGAAGAATTGGTGAAACAGGAACCGGACATGGCACGAAATGAGCCGGATAATGATGATAAGGCTGAACAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2653532‑2653669
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGGCATATTCGGAACTGTGTGATGAACGTTTTCTTCTTCCATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATATTCGGATAATTTGCATTTTCCATATTACTCATAGCCTCCTG  >  NZ_CP034943/2653670‑2653789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.