New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP034943	= 2653801	327 (1.280)	3 (0.010)	3/246	NT	0.94%	coding (466/1161 nt)	safA	spore coat assembly protein SafA
	?	NZ_CP034943	2653808 =	314 (1.260)					coding (459/1161 nt)	safA	spore coat assembly protein SafA
Rejected: Frequency below/above cutoff threshold.

GCTGAACAGCCGGCATATTCGGAACTGTGTGATGAACGTTTTCTTCTTCCATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATATTCGGATAATTTGCATTTTCCATATTACTCATAGCCTCCTGTTGTGGAAATTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2653660‑2653801 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgGTCTTGCATGTGATACATATGATCCATATTTCCGTCATGATGTTTTTTCGGCTCCTCCGGTTTAGGGGGCGACCAAGGCTGGAAAAGCTGTTTCATATCATAATAATCGTTCACATCAGCTTCAGGATACACATCTTCC  >  NZ_CP034943/2653808‑2653946                                                                                                                                                                                                                                                                                           GCTGAACAGCCGGCATATTCGGAACTGTGTGATGAACGTTTTCTTCTTCCATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATATTCGGATAATTTGCATTTTCCATATTACTCATAGCCTCCTGTTGTGGAAAT                                                                                                                                               <  1:3130457/140‑1                                                         CCTACCTCTGGCGCCTTTGGCATATTAGGCATATTCGGATAATTTGCATTTTCCATATTACTCATAGCCTCCTGTTGTGGAAATTGGTCTTGCATATGATACATATGATCCATATTTCCGTCATGATGTTTTTTCGGCTC                                                                                       >  1:3922931/1‑140                                                                                 TTAGGCATATTCGGATAATTTGCATTTTCCAAATTACTCATAGACTCATGTTGTGGAACTTTGTCTTGCATATTAGACATATGATCCATATTTCCGTCATGATTTTTTTTCGGCTCCTCCGGTTTAGGAGGCGACCAAGG                                                               >  2:794519/1‑140                                                                                          TTCGGATAATTTGCATTTTCCATATTACTCATAGCCGCCTGTTGTGGAAATTGGTCTTGCATATGATACATATGATCCATATTTCCGTCATGATGTTTTTTCGGCTCCTCCGGTTTAGGAGGCGACCAAGGCTGGAAAAG                                                      >  1:3330516/1‑140                                                                                                                                           cgTGGTATTGCATGTGATACATTTGATCCATATTTCCGTCATGATGTTTTTTCGGCTCCTCCGGTTTAGGGGGCGCCCAAGGCTGGAAAAGCTGTTTCATATCATAATAATCGTTCACATCAGCTTCAGGATACACATCT     <  2:2091156/138‑1                                                                                                                                             TGGTCTTGCATGTGATACATATGATCCATATTTCCGTCATGATGTTTTTTCGGCTCCTCCGGTTTAGGGGGCGACCAAGGCTGGAAAAGCTGTTTCATATCATAATAATCGTTCACATCAGCTTCAGGATACACATCTTC   <  2:1147342/140‑1                                                                                                                                             TGGTCTTGCATGTGATACATATGATCCATATGTCCGTCATGATGTTTTTTCGGCTCCTCCGGTTTAGGGGGCGACCAAGGCTGGAAAAGCTGTTTCATATCATAATAATCGTTCACATCAGCTTCAGGATACACATCTTC   <  2:3925307/140‑1                                                                                                                                              GGTCTTGCATGTGATACATATGATCCATATTTCCGTCATGATGTTTTTTCGGCTCCTCCGGTTTAGGGGGCGACCAAGGCTGGAAAAGCTGTTTCATATCATAATAATCGTTCACATCAGCTTCAGGATACACATCTTCC  >  1:131629/1‑140                                                                                                                                                                                                                                                                                           GCTGAACAGCCGGCATATTCGGAACTGTGTGATGAACGTTTTCTTCTTCCATACCGCCTACCTCTGGCGCCTTTGGCATATTAGGCATATTCGGATAATTTGCATTTTCCATATTACTCATAGCCTCCTGTTGTGGAAATTG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/2653660‑2653801 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttgGTCTTGCATGTGATACATATGATCCATATTTCCGTCATGATGTTTTTTCGGCTCCTCCGGTTTAGGGGGCGACCAAGGCTGGAAAAGCTGTTTCATATCATAATAATCGTTCACATCAGCTTCAGGATACACATCTTCC  >  NZ_CP034943/2653808‑2653946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.