New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NZ_CP034943	= 1550631	265 (0.840)	6 (0.020) +CCGTCG	3/240	NT	2.3%	intergenic (+8/‑126)	pbpB/EO946_RS08110	penicillin‑binding protein 2B/stage V sporulation protein D
	?	NZ_CP034943	1550655 =	261 (0.830)					intergenic (+32/‑102)	pbpB/EO946_RS08110	penicillin‑binding protein 2B/stage V sporulation protein D
Rejected: Frequency below/above cutoff threshold.

ACAAGGCTATGCTGTCAGCCAAAGCATCAAGAAGGACAAAGAAATAAAAGGGAAAAACGTTATCAAGGTTAAGTTTAAAAACCCTGATTAAAAAGAAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/1550533‑1550631 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTATGCAGGCTTTCTTTTTTTATACCTTCAGAGGAGCATCGTTCTACCTGTCCAATTACAGGCATAAAATGAAACAAGCC  >  NZ_CP034943/1550655‑1550734                                                                                                    ||||||                                                                                 ACAAGGCTACGCTGTCAGCCAAAGTGTTAAGAAAGACAAAGAAATCAAAGACAAAACCGTAATCAAGGTTAAGTTTAAAAATCCTGATTAAAAAGAAAGCCGTCGTTATGCAGGCTTTCTTTTTTTATGCCTTCAGAGGA                                               >  1:2553105/1‑140 ACAAGGCTACGCTGTCAGCCAAAGTGTTAAGAAAGACAAAGAAATCAAAGACAAAACCGTAATCAAGGTTAAGTTTAAAAATCCTGATTAAAAAGAAAGCCGTCGTTATGCAGGCTTTCTTTTTTTATGCCTTCAGAGGA                                               >  1:4309296/1‑140                                   GACAAAGAAATCAAAGACAAAACCGTAATCAAGGTTAAGTTTAAAAATCCTGATTAAAAAGAAAGCCGTCGTTATGCAGGCTTTCTTTTTTTATGCCTTCAGAGGAGCATCGTTCTACCTGTCCAAATTCAGGCATAAAA             >  2:4639409/1‑140                                   GACAAAGAAATCAAAGACAAAACCGTAATCAAGGTTAAGTTTAAAAATCCTGATTAAAAAGAAAGCCGTCGTTATGCAGGCTTTCTTTTTTTATGCCTTCAGAGGAGCATCGTTCTACCTGTCCAAATTCAGGCATAAAA             >  2:4894119/1‑140                                              caaagacAAAACCGTAATCAAGGTTAAGTTTAAAAATCCTGATTAAAAAGAAAGCCGTCGTTATGCAGGCTTTCTTTTTTTATGCCTTCAGAGGAGCATCGTTCTACCTGTCCAAATTCAGGCATAAAATGAAACAAGCC  <  2:2553105/133‑1                                              caaagacAAAACCGTAATCAAGGTTAAGTTTAAAAATCCTGATTAAAAAGAAAGCCGTCGTTATGCAGGCTTTCTTTTTTTATGCCTTCAGAGGAGCATCGTTCTACCTGTCCAAATTCAGGCATAAAATGAAACAAGCC  <  2:4309296/133‑1                                                                                                    ||||||                                                                                 ACAAGGCTATGCTGTCAGCCAAAGCATCAAGAAGGACAAAGAAATAAAAGGGAAAAACGTTATCAAGGTTAAGTTTAAAAACCCTGATTAAAAAGAAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NZ_CP034943/1550533‑1550631 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTATGCAGGCTTTCTTTTTTTATACCTTCAGAGGAGCATCGTTCTACCTGTCCAATTACAGGCATAAAATGAAACAAGCC  >  NZ_CP034943/1550655‑1550734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.