Predicted mutation
evidence seq id position mutation annotation gene description
JC JC CP009974 3,288,192 ISS12 (–) +7 bp :: +A intergenic (‑185/‑66) RPPX_14645 ← / → RPPX_14650 antibiotic transporter/TetR family transcriptional regulator

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009974 2756352 =NA (NA)331 (1.220) 192/300 0.0 100% noncoding (2595/2595 nt) ISS12 repeat region
?CP009974 = 3288198 0 (0.000)intergenic (‑191/‑66) RPPX_14645/RPPX_14650 antibiotic transporter/TetR family transcriptional regulator
* ? CP009974 3288192 =0 (0.000)280 (1.030) 170/300 0.2 100% intergenic (‑185/‑72) RPPX_14645/RPPX_14650 antibiotic transporter/TetR family transcriptional regulator
?CP009974 3486773 = NA (NA)intergenic (+359/‑203) RPPX_15550/RPPX_15555 RNA polymerase sigma factor/integrase

ACGAAGATGGTGGGGATGTTGTACTGCGCTGCAGTCCACAACGCACTGATGCTGTAGTTCGCCGAACCGTCTGCGGATTCCACGCCATTCGGACACTCAGCCCACGCTGATCCGGACACCTGTTCCACGCTCATTCGGACAGGCAGTCGGAGCGCAGCGACGC  >  CP009974/2756281‑2756443
                                                                       |                                                                                           
aCGAAGATGGTGGGGATGTTGTACTGCGCTGCAGTCCACAACGCACTGATGCTGTAGTT‑‑‑‑‑‑‑‑‑‑‑‑TGCGGATTCCACGCCATTCGGACACTCAGCCCACGCTGATCCGGACACCTGTTCCACGCTCATTCGGACAGGCCGTAGGCGCGCCGCGACGc  >  2:3902181/1‑151 (MQ=14)
                                                                       |                                                                                           
ACGAAGATGGTGGGGATGTTGTACTGCGCTGCAGTCCACAACGCACTGATGCTGTAGTTCGCCGAACCGTCTGCGGATTCCACGCCATTCGGACACTCAGCCCACGCTGATCCGGACACCTGTTCCACGCTCATTCGGACAGGCAGTCGGAGCGCAGCGACGC  >  CP009974/2756281‑2756443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.