Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	U00096	1,618,499	Δ7 bp	coding (404‑410/666 nt)	marC ←	inner membrane protein MarC

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	U00096	1618499	1618505	7	56 [0]	[0] 55	marC	inner membrane protein MarC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	U00096	= 1618498	0 (0.000)	55 (0.620)	40/290	2.2	100%	coding (411/666 nt)	marC	inner membrane protein MarC
	?	U00096	1618506 =	0 (0.000)					coding (403/666 nt)	marC	inner membrane protein MarC

CGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGA  >  U00096/1618361‑1618649                                                                                                                                                  |                                                                                                                                                cacaagccgcataattgcgccggagctgcgtaggctgccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTg                                                                                                                                                <  1:471732‑M2/2‑1 (MQ=255)   ccagccgcataattgcgccggagctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGt                                                                                                                                              >  1:471775‑M2/144‑147 (MQ=255)    cagccgcataattgcgccggagctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTc                                                                                                                                            <  1:471733‑M2/6‑1 (MQ=255)        cgcataattgcgccggagctgcgtaggcgtccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCgg                                                                                                                                        <  2:471734‑M2/10‑1 (MQ=255)               ttgcgccggagctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCa                                                                                                                                 >  1:471773‑M2/132‑148 (MQ=255)                     cggagctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACtt                                                                                                                           <  1:471735‑M2/23‑1 (MQ=255)                     cggagctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACtt                                                                                                                           <  2:471736‑M2/23‑1 (MQ=255)                      ggagctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatccgcggctgcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACtt                                                                                                                           <  1:471737‑M2/23‑1 (MQ=255)                      ggagctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTg                                                                                                                          <  2:471738‑M2/24‑1 (MQ=255)                          ctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCAt                                                                                                                      <  2:471739‑M2/28‑1 (MQ=255)                          ctgcgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCAt                                                                                                                      <  2:471740‑M2/28‑1 (MQ=255)                             cgtaggcttccccacaaaatatccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatccgcgaaggtggaactctgcagcactgaggacgccgagctaataatcTGCTTCCCGAACCGGCAGTACTTGGCATTGc                                                                                                                   >  2:471799‑M2/118‑148 (MQ=255)                             cgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGc                                                                                                                   >  2:471741‑M2/118‑148 (MQ=255)                             cgtaggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGc                                                                                                                   >  1:471778‑M2/118‑148 (MQ=255)                              gtaggcttccccacaaaataaccgccaccaggaaaaagatcatcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgatctaataatcTGGTTCCCGGACCGGCAGTACtt                                                                                                                           <  1:471743‑M2/23‑1 (MQ=255)                              gtaggcttccccacaaaataaccgccaccaggaaaaagagcatcggcggcgcaaccatcagtacccaatcatctaaggtttaactctgactcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACtt                                                                                                                           <  1:471744‑M2/23‑1 (MQ=255)                               taggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGCCCGGCAGTACTTTGCATTGCCa                                                                                                                 >  2:471765‑M2/116‑148 (MQ=255)                                aggcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAg                                                                                                                <  1:471746‑M2/34‑1 (MQ=255)                                  gcttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCg                                                                                                              <  1:692372‑M2/36‑1 (MQ=255)                                   cttccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCgg                                                                                                             >  2:471785‑M2/112‑148 (MQ=255)                                      ccccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGcac                                                                                                          >  1:471800‑M2/109‑148 (MQ=255)                                        ccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACaa                                                                                                        >  2:471777‑M2/107‑148 (MQ=255)                                        ccacaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGCTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACaa                                                                                                        >  1:471786‑M2/107‑148 (MQ=255)                                         cacaaaataaccgccacaaggaaaaagatcagcggcggcgcaaccatcagtacacaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACAGTCAGTACTTGGCATTGCCAGCGGCACaaa                                                                                                       >  1:471764‑M2/106‑148 (MQ=255)                                          acaaaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAg                                                                                                      >  1:471788‑M2/105‑148 (MQ=255)                                             aaataaccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCa                                                                                                   >  2:471793‑M2/102‑148 (MQ=255)                                                  accgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATAtt                                                                                              <  2:471747‑M2/52‑1 (MQ=255)                                                   ccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTg                                                                                             <  2:471749‑M2/53‑1 (MQ=255)                                                   ccgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTg                                                                                             <  2:471748‑M2/53‑1 (MQ=255)                                                    cgccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTgg                                                                                            >  2:471791‑M2/95‑148 (MQ=255)                                                     gccaccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGc                                                                                           <  2:471750‑M2/55‑1 (MQ=255)                                                       caccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCAc                                                                                         <  2:471751‑M2/57‑1 (MQ=255)                                                         ccaggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTg                                                                                       >  2:471795‑M2/90‑148 (MQ=255)                                                          caggaaaaagatcagcggcggcgccaccatcagtacccaatcagcgaaggttgatctctgacgcactgtggacgccgagctattaatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCCCTgg                                                                                      >  1:471762‑M2/89‑148 (MQ=255)                                                          caggaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTgg                                                                                      >  2:471770‑M2/89‑148 (MQ=255)                                                             gaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAatat                                                                                               >  1:471752‑M2/86‑136 (MQ=255)                                                             gaaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGtt                                                                                   <  2:471753‑M2/63‑1 (MQ=255)                                                              aaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaatgttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGAAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTc                                                                                  <  1:471755‑M2/64‑1 (MQ=255)                                                              aaaaagatcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTc                                                                                  <  2:471754‑M2/64‑1 (MQ=255)                                                                    atccgcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTc                                                                            <  2:471757‑M2/70‑1 (MQ=255)                                                                    atcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTc                                                                            <  2:471758‑M2/70‑1 (MQ=255)                                                                    atcagcggcggcgcaaccatcagtacccaatcagcgaaggttgaacgctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTc                                                                            <  1:471756‑M2/70‑1 (MQ=255)                                                                      cagcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTcc                                                                           <  1:471759‑M2/71‑1 (MQ=255)                                                                       agcggcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAg                                                                         >  1:471790‑M2/76‑148 (MQ=255)                                                                           gcggcgcaaccatcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGctct                                                                     >  2:471792‑M2/72‑148 (MQ=255)                                                                                      atcagtacccaatcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCt                                                          >  2:471796‑M2/61‑148 (MQ=255)                                                                                                 atcagcgaaggttgaactctgacgcactgtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCg                                               >  1:471767‑M2/50‑148 (MQ=255)                                                                                                                             gtggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGaaa                   >  2:471787‑M2/22‑148 (MQ=255)                                                                                                                              tggacgccgagctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAag                  >  2:471768‑M2/21‑148 (MQ=255)                                                                                                                                       agctaataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGa         >  1:471783‑M2/12‑148 (MQ=255)                                                                                                                                           aataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAAc     >  2:471782‑M2/8‑148 (MQ=255)                                                                                                                                            ataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGCGCATCCGAAAAcc    >  2:471813‑M2/7‑148 (MQ=255)                                                                                                                                            ataatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAAcc    >  1:471801‑M2/7‑148 (MQ=255)                                                                                                                                              aatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGa  >  2:471802‑M2/5‑148 (MQ=255)                                                                                                                                              aatcTGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGa  >  2:471803‑M2/5‑148 (MQ=255)                                                                                                                                                  |                                                                                                                                                CGCATAATTGCGCCGGAGCTGCGTAGGCTTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGA  >  U00096/1618361‑1618649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑