breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG46.3% 314.0 / 123.5 273intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC39.6% 349.4 / 55.9 220G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123062,758,9090TG29.0% 444.7 / 20.7 194G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP012306644,4170GA28.2% 975.4 / inf 571intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP012306800,6750AC28.2% 488.2 / 40.3 227T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123063,938,8870CT27.0% 504.6 / 135.8 241S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,527,3680AC26.4% 656.6 / 43.3 273L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306257,1370TG26.2% 372.9 / 17.4 191L496V (TTG→GTG) betTcholine transporter of high affinity
*AP0123062,714,5550TG25.7% 591.9 / 25.3 262T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP012306406,6770AG25.5% 478.3 / 22.4 200K294E (AAA→GAA) hemHferrochelatase
*AP012306341,3770TG25.0% 202.6 / 17.3 113G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123062,558,0710AC25.0% 571.7 / 33.3 249V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP012306524,2890TG24.2% 401.6 / 13.1 166N4T (AAC→ACC) citDcitrate lyase, acyl carrier (gamma) subunit
*AP0123063,412,0490TG23.6% 864.4 / 39.3 351intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP0123061,128,4120TG23.2% 745.4 / 45.1 299V29G (GTG→GGG) yciOconserved protein
*AP0123061,854,8510AC22.9% 729.1 / 65.5 297T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306935,0540GT22.9% 239.4 / 24.9 83intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123061,226,5570AG22.9% 417.1 / 58.9 175G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,212,7530AC22.2% 329.9 / 34.6 135Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123062,680,3450AC22.1% 444.2 / 16.1 200T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit

Marginal new junction evidence (lowest skew 10 of 422 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 644102 =482 (1.530)37 (0.120)
+GAAG
14/290 9.1 8.2% noncoding (1/76 nt) lysZ tRNA‑Lys
?AP012306 = 2073420 368 (1.170)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 1154242 =558 (1.770)13 (0.040) 13/294 9.4 2.3% coding (95/1128 nt) yciW predicted oxidoreductase
?AP012306 1154260 = 564 (1.810)coding (77/1128 nt) yciW predicted oxidoreductase
* ? AP012306 = 1092334292 (0.930)15 (0.050) 11/258 9.4 5.3% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 287 (1.050)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 1325578333 (1.060)11 (0.040) 11/274 9.6 3.5% coding (158/666 nt) marC predicted transporter
?AP012306 = 1325566 302 (1.040)coding (170/666 nt) marC predicted transporter
* ? AP012306 644310 =365 (1.160)99 (0.320)
+GAAG
12/290 9.6 21.7% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 368 (1.170)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 = 215364NA (NA)12 (0.040) 12/288 9.6 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 643287 =414 (1.310)33 (0.110)
+GAAG
10/290 10.1 8.0% noncoding (1/76 nt) lysT tRNA‑Lys
?AP012306 = 2073420 368 (1.170)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 = 1007301359 (1.140)9 (0.030) 9/274 10.2 2.7% coding (503/1200 nt) lolC outer membrane‑specific lipoprotein transporter subunit
?AP012306 = 1007289 327 (1.130)coding (491/1200 nt) lolC outer membrane‑specific lipoprotein transporter subunit
* ? AP012306 = 3131909201 (0.640)9 (0.030) 9/284 10.3 4.6% coding (2235/2619 nt) bcsA cellulose synthase, catalytic subunit
?AP012306 = 3131925 184 (0.610)coding (2219/2619 nt) bcsA cellulose synthase, catalytic subunit
* ? AP012306 = 1092512375 (1.190)18 (0.070) 8/258 10.3 5.6% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 287 (1.050)noncoding (66/85 nt) tyrT tRNA‑Tyr