breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 46.3% | 314.0 / 123.5 | 273 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 1,944,288 | 0 | A | C | 39.6% | 349.4 / 55.9 | 220 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 2,758,909 | 0 | T | G | 29.0% | 444.7 / 20.7 | 194 | G33G (GGT→GGG) | agaD | N‑acetylgalactosamine‑specific enzyme IID component of PTS |
* | AP012306 | 644,417 | 0 | G | A | 28.2% | 975.4 / inf | 571 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 800,675 | 0 | A | C | 28.2% | 488.2 / 40.3 | 227 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 3,938,887 | 0 | C | T | 27.0% | 504.6 / 135.8 | 241 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 3,527,368 | 0 | A | C | 26.4% | 656.6 / 43.3 | 273 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 257,137 | 0 | T | G | 26.2% | 372.9 / 17.4 | 191 | L496V (TTG→GTG) | betT | choline transporter of high affinity |
* | AP012306 | 2,714,555 | 0 | T | G | 25.7% | 591.9 / 25.3 | 262 | T175P (ACC→CCC) | ygjV | conserved inner membrane protein |
* | AP012306 | 406,677 | 0 | A | G | 25.5% | 478.3 / 22.4 | 200 | K294E (AAA→GAA) | hemH | ferrochelatase |
* | AP012306 | 341,377 | 0 | T | G | 25.0% | 202.6 / 17.3 | 113 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 2,558,071 | 0 | A | C | 25.0% | 571.7 / 33.3 | 249 | V302G (GTA→GGA) | visC | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
* | AP012306 | 524,289 | 0 | T | G | 24.2% | 401.6 / 13.1 | 166 | N4T (AAC→ACC) | citD | citrate lyase, acyl carrier (gamma) subunit |
* | AP012306 | 3,412,049 | 0 | T | G | 23.6% | 864.4 / 39.3 | 351 | intergenic (+25/‑122) | proM/aslB | tRNA‑Pro/predicted regulator of arylsulfatase activity |
* | AP012306 | 1,128,412 | 0 | T | G | 23.2% | 745.4 / 45.1 | 299 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 1,854,851 | 0 | A | C | 22.9% | 729.1 / 65.5 | 297 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 935,054 | 0 | G | T | 22.9% | 239.4 / 24.9 | 83 | intergenic (+24/‑1406) | putP/ycdO | proline:sodium symporter/conserved protein |
* | AP012306 | 1,226,557 | 0 | A | G | 22.9% | 417.1 / 58.9 | 175 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 2,212,753 | 0 | A | C | 22.2% | 329.9 / 34.6 | 135 | Y241D (TAT→GAT) | hcaT | predicted 3‑phenylpropionic transporter |
* | AP012306 | 2,680,345 | 0 | A | C | 22.1% | 444.2 / 16.1 | 200 | T184P (ACC→CCC) | ttdA | L‑tartrate dehydratase, alpha subunit |
Marginal new junction evidence (lowest skew 10 of 422 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | 644102 = | 482 (1.530) | 37 (0.120) +GAAG |
14/290 | 9.1 | 8.2% | noncoding (1/76 nt) | lysZ | tRNA‑Lys |
? | AP012306 | = 2073420 | 368 (1.170) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | 1154242 = | 558 (1.770) | 13 (0.040) | 13/294 | 9.4 | 2.3% | coding (95/1128 nt) | yciW | predicted oxidoreductase |
? | AP012306 | 1154260 = | 564 (1.810) | coding (77/1128 nt) | yciW | predicted oxidoreductase | |||||
* | ? | AP012306 | = 1092334 | 292 (0.930) | 15 (0.050) | 11/258 | 9.4 | 5.3% | coding (198/276 nt) | ychS | predicted protein |
? | AP012306 | 1092985 = | 287 (1.050) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr | |||||
* | ? | AP012306 | = 1325578 | 333 (1.060) | 11 (0.040) | 11/274 | 9.6 | 3.5% | coding (158/666 nt) | marC | predicted transporter |
? | AP012306 | = 1325566 | 302 (1.040) | coding (170/666 nt) | marC | predicted transporter | |||||
* | ? | AP012306 | 644310 = | 365 (1.160) | 99 (0.320) +GAAG |
12/290 | 9.6 | 21.7% | noncoding (1/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073420 | 368 (1.170) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | = 215364 | NA (NA) | 12 (0.040) | 12/288 | 9.6 | NA | noncoding (745/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) |
? | AP012306 | = 215372 | NA (NA) | noncoding (753/2905 nt) | rrlH | 23S ribosomal RNA (rrlH) | |||||
* | ? | AP012306 | 643287 = | 414 (1.310) | 33 (0.110) +GAAG |
10/290 | 10.1 | 8.0% | noncoding (1/76 nt) | lysT | tRNA‑Lys |
? | AP012306 | = 2073420 | 368 (1.170) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | = 1007301 | 359 (1.140) | 9 (0.030) | 9/274 | 10.2 | 2.7% | coding (503/1200 nt) | lolC | outer membrane‑specific lipoprotein transporter subunit |
? | AP012306 | = 1007289 | 327 (1.130) | coding (491/1200 nt) | lolC | outer membrane‑specific lipoprotein transporter subunit | |||||
* | ? | AP012306 | = 3131909 | 201 (0.640) | 9 (0.030) | 9/284 | 10.3 | 4.6% | coding (2235/2619 nt) | bcsA | cellulose synthase, catalytic subunit |
? | AP012306 | = 3131925 | 184 (0.610) | coding (2219/2619 nt) | bcsA | cellulose synthase, catalytic subunit | |||||
* | ? | AP012306 | = 1092512 | 375 (1.190) | 18 (0.070) | 8/258 | 10.3 | 5.6% | intergenic (+100/+3) | ychS/tpr | predicted protein/predicted protamine‑like protein |
? | AP012306 | 1092985 = | 287 (1.050) | noncoding (66/85 nt) | tyrT | tRNA‑Tyr |