breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 24 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG44.9% 399.6 / 90.7 274intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC35.6% 390.0 / 73.9 232G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP012306935,0540GT34.2% 143.6 / 59.6 73intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123063,938,8870CT34.2% 378.7 / 179.8 243S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,372,7010TG32.9% 138.7 / 19.9 79noncoding (54/76 nt)gltUtRNA‑Glu
*AP012306644,4170GA31.4% 835.6 / inf 555intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP012306341,3770TG29.1% 189.9 / 13.1 103G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,527,3680AC28.1% 593.6 / 54.2 255L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123061,226,5570AG25.9% 370.9 / 73.4 170G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,903,7910AG24.8% 613.9 / 87.1 270E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123062,758,9090TG24.7% 606.1 / 18.1 228G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123063,182,8610AC24.6% 339.3 / 13.6 130L198F (TTA→TTCyiaNpredicted transporter
*AP012306800,6750AC24.1% 665.1 / 28.8 261T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306257,1370TG23.9% 396.5 / 12.2 184L496V (TTG→GTG) betTcholine transporter of high affinity
*AP0123061,854,8510AC22.9% 691.6 / 48.8 263T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP012306406,6770AG22.4% 531.8 / 26.4 211K294E (AAA→GAA) hemHferrochelatase
*AP012306202,4170TG22.2% 679.8 / 23.2 271V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123061,128,4120TG22.2% 869.7 / 33.3 320V29G (GTG→GGG) yciOconserved protein
*AP0123063,324,4330TA22.1% 506.7 / 68.2 199intergenic (+2/+52)yieF/yieGchromate reductase, Class I, flavoprotein/predicted inner membrane protein
*AP0123062,558,0710AC21.8% 687.3 / 26.7 262V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain

Marginal new junction evidence (lowest skew 10 of 31 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 215364NA (NA)8 (0.030) 8/288 12.8 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 2718234 =374 (1.200)7 (0.020) 7/274 12.9 1.9% intergenic (‑160/‑203) uxaC/exuT uronate isomerase/hexuronate transporter
?AP012306 2718246 = 365 (1.270)intergenic (‑172/‑191) uxaC/exuT uronate isomerase/hexuronate transporter
* ? AP012306 = 2275071207 (0.660)43 (0.160)
+GTATCAGTCTGCTTCGCAAG
5/258 13.5 14.6% noncoding (1/76 nt) gltW tRNA‑Glu
?AP012306 = 3597478 375 (1.200)intergenic (+65/‑107) rrsB/gltT 16S ribosomal RNA (rrnB)/tRNA‑Glu
* ? AP012306 = 3429575390 (1.250)6 (0.020) 6/288 13.6 1.6% coding (695/765 nt) yigE predicted protein
?AP012306 = 3429585 374 (1.240)coding (685/765 nt) yigE predicted protein
* ? AP012306 = 727361269 (0.860)5 (0.020) 5/282 14.0 2.0% coding (502/750 nt) moeB molybdopterin synthase sulfurylase
?AP012306 = 727373 246 (0.830)coding (490/750 nt) moeB molybdopterin synthase sulfurylase
* ? AP012306 3353383 =269 (0.860)5 (0.020) 5/290 14.1 1.8% coding (1464/1890 nt) gidA glucose‑inhibited cell‑division protein
?AP012306 3353403 = 285 (0.940)coding (1444/1890 nt) gidA glucose‑inhibited cell‑division protein
* ? AP012306 2939858 =257 (0.820)5 (0.020) 5/290 14.1 1.9% coding (1142/2103 nt) yhfK conserved inner membrane protein
?AP012306 2939902 = 268 (0.880)coding (1186/2103 nt) yhfK conserved inner membrane protein
* ? AP012306 = 2359634210 (0.670)5 (0.020) 5/288 14.1 2.4% coding (143/1827 nt) hycC hydrogenase 3, membrane subunit
?AP012306 = 2359655 205 (0.680)coding (122/1827 nt) hycC hydrogenase 3, membrane subunit
* ? AP012306 3111802 =373 (1.190)4 (0.010) 4/274 14.3 1.1% coding (554/972 nt) yhjC predicted DNA‑binding transcriptional regulator
?AP012306 3111814 = 345 (1.200)coding (566/972 nt) yhjC predicted DNA‑binding transcriptional regulator
* ? AP012306 = 3089215379 (1.210)3 (0.010) 3/248 14.4 0.92% intergenic (+235/‑642) gor/arsR glutathione oxidoreductase/DNA‑binding transcriptional regulator
?AP012306 3089646 = 333 (1.280)intergenic (+666/‑211) gor/arsR glutathione oxidoreductase/DNA‑binding transcriptional regulator