BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	AP012306	1,322,972	Δ2,926 bp		[yneK]–marC	[yneK], ydeA, marC

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	AP012306	1322972	1325897	2926	397 [1]	[1] 396	[yneK]–marC	[yneK],ydeA,marC

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	AP012306	= 1322971	1 (0.000)	395 (1.170)	219/298	0.0	99.8%	coding (382/1116 nt)	yneK	predicted protein
*	?	AP012306	1325898 =	1 (0.000)	395 (1.170)	219/298	0.0	99.8%	intergenic (‑163/‑49)	marC/marR	predicted transporter/DNA‑binding transcriptional repressor

Only 100 of 396 total aligned reads displayed.

CCTGTGTATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGT  >  AP012306/1325749‑1326046

cacgctatacaatgaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttc                                                                                                                                                      <  1:3254609‑M2/1‑1 (MQ=255)

 acgctatacaatgaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttcc                                                                                                                                                     <  2:4256899‑M2/2‑1 (MQ=255)

 acgctatacaatgaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttcc                                                                                                                                                     <  1:2229391‑M2/2‑1 (MQ=255)

  cgctatacaatgaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCt                                                                                                                                                    <  2:1917548‑M2/3‑1 (MQ=255)

      atacaatgaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGc                                                                                                                                                >  2:3192089‑M2/144‑150 (MQ=255)

          aatgaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAata                                                                                                                                            <  2:639833‑M2/11‑1 (MQ=255)

            tgaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAAtatt                                                                                                                                          >  1:2844290‑M2/138‑150 (MQ=255)

             gaaaaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAAtatta                                                                                                                                         >  1:1225287‑M2/137‑150 (MQ=255)

                aaggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATcc                                                                                                                                      >  1:41417‑M2/134‑150 (MQ=255)

                  ggttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATcccc                                                                                                                                    <  2:1167759‑M2/19‑1 (MQ=255)

                   gttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCt                                                                                                                                   <  2:3777783‑M2/20‑1 (MQ=255)

                   gttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCt                                                                                                                                   >  2:2596616‑M2/131‑150 (MQ=255)

                    ttcagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTg                                                                                                                                  >  1:783508‑M2/130‑150 (MQ=255)

                      cagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCa                                                                                                                                >  2:3469514‑M2/128‑150 (MQ=255)

                      cagtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCa                                                                                                                                >  2:286642‑M2/128‑150 (MQ=255)

                       agtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCaa                                                                                                                               >  1:4345587‑M2/127‑150 (MQ=255)

                       agtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCaa                                                                                                                               >  1:1525222‑M2/127‑150 (MQ=255)

                        gtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAAc                                                                                                                              >  2:409156‑M2/126‑150 (MQ=255)

                        gtttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAAc                                                                                                                              <  1:3343504‑M2/25‑1 (MQ=255)

                         tttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACt                                                                                                                             >  2:4415823‑M2/125‑150 (MQ=255)

                         tttaaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACt                                                                                                                             >  1:3305793‑M2/125‑150 (MQ=255)

                            aaactcacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAAt                                                                                                                          <  2:2788018‑M2/29‑1 (MQ=255)

                                 catttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACtt                                                                                                                     <  2:679177‑M2/34‑1 (MQ=255)

                                 cacttatcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACtt                                                                                                                     <  2:3394660‑M2/34‑1 (MQ=255)

                                       tcccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAgg                                                                                                               <  2:2451867‑M2/40‑1 (MQ=255)

                                         ccgccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGc                                                                                                             <  2:17341‑M2/42‑1 (MQ=255)

                                           gccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCaa                                                                                                           <  2:4284648‑M2/44‑1 (MQ=255)

                                            ccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAAc                                                                                                          >  2:3579541‑M2/106‑150 (MQ=255)

                                            ccaggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAAc                                                                                                          <  2:2950038‑M2/45‑1 (MQ=255)

                                              aggaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTa                                                                                                        >  2:3078087‑M2/104‑150 (MQ=255)

                                                gaatggaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAAt                                                                                                      <  2:1458847‑M2/49‑1 (MQ=255)

                                                     gaagacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGaa                                                                                                 >  1:1930695‑M2/97‑150 (MQ=255)

                                                       agacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGaaaa                                                                                               <  2:327504‑M2/56‑1 (MQ=255)

                                                       agacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGaaaa                                                                                               >  1:3905180‑M2/95‑150 (MQ=255)

                                                        gacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAg                                                                                              <  1:1958785‑M2/57‑1 (MQ=255)

                                                        gacacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAg                                                                                              <  1:4069720‑M2/57‑1 (MQ=255)

                                                          cacaaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTa                                                                                            <  2:3676942‑M2/59‑1 (MQ=255)

                                                            caaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTAcc                                                                                          >  1:558114‑M2/90‑150 (MQ=255)

                                                             aaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTCCTTGCCAGGGCAACTAATGTGAAAAGTACCa                                                                                         <  2:1796401‑M2/62‑1 (MQ=255)

                                                             aaagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCa                                                                                         >  1:1364915‑M2/89‑150 (MQ=255)

                                                              aagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAg                                                                                        >  1:835207‑M2/88‑150 (MQ=255)

                                                              aagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAg                                                                                        >  1:3054346‑M2/88‑150 (MQ=255)

                                                              aagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAg                                                                                        <  1:2678311‑M2/63‑1 (MQ=255)

                                                              aagaaaatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAg                                                                                        <  1:4176728‑M2/63‑1 (MQ=255)

                                                                   aatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATc                                                                                   <  1:3793158‑M2/68‑1 (MQ=255)

                                                                   aatattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATc                                                                                   >  1:604337‑M2/83‑150 (MQ=255)

                                                                    atattgaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCt                                                                                  >  2:2116383‑M2/82‑150 (MQ=255)

                                                                         gaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCa                                                                             >  1:3967216‑M2/77‑150 (MQ=255)

                                                                         gaatttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCa                                                                             >  1:3097932‑M2/77‑150 (MQ=255)

                                                                           atttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAAt                                                                           >  1:1138693‑M2/75‑150 (MQ=255)

                                                                            tttcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGAACCAGCGATCTGTTCATTg                                                                          >  1:3189198‑M2/74‑150 (MQ=255)

                                                                              tcaggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGaa                                                                        >  2:745176‑M2/72‑150 (MQ=255)

                                                                               caggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGaaa                                                                       >  2:417093‑M2/71‑150 (MQ=255)

                                                                                aggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAat                                                                      <  1:4488997‑M2/81‑1 (MQ=255)

                                                                                 ggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAatt                                                                     <  1:1092889‑M2/82‑1 (MQ=255)

                                                                                 ggtagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAatt                                                                     <  2:2388972‑M2/82‑1 (MQ=255)

                                                                                   tagtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAattat                                                                   >  1:457573‑M2/67‑150 (MQ=255)

                                                                                    agtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAattatt                                                                  >  1:1561695‑M2/66‑150 (MQ=255)

                                                                                    agtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAattatt                                                                  >  1:1605679‑M2/66‑150 (MQ=255)

                                                                                    agtaataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAattatt                                                                  <  2:4510314‑M2/85‑1 (MQ=255)

                                                                                        ataaatttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCAt                                                              <  1:2750543‑M2/89‑1 (MQ=255)

                                                                                            atttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTggg                                                          >  1:3852705‑M2/58‑150 (MQ=255)

                                                                                             tttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGt                                                         <  1:2431210‑M2/94‑1 (MQ=255)

                                                                                              ttaagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTc                                                        >  2:574935‑M2/56‑150 (MQ=255)

                                                                                               taagccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCg                                                       <  2:1341266‑M2/96‑1 (MQ=255)

                                                                                                  gccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCtt                                                    >  1:1815612‑M2/52‑150 (MQ=255)

                                                                                                  gccctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCtt                                                    >  1:2149500‑M2/52‑150 (MQ=255)

                                                                                                    cctatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTaa                                                  <  1:3407894‑M2/101‑1 (MQ=255)

                                                                                                      tatttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATc                                                <  1:1741995‑M2/103‑1 (MQ=255)

                                                                                                       atttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATcc                                               <  1:4288928‑M2/104‑1 (MQ=255)

                                                                                                        tttatttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCa                                              <  1:3618399‑M2/105‑1 (MQ=255)

                                                                                                           atttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                                           <  1:4019958‑M2/108‑1 (MQ=255)

                                                                                                             ttagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATgg                                         >  1:2525054‑M2/41‑150 (MQ=255)

                                                                                                              tagataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGt                                        >  2:3360237‑M2/40‑150 (MQ=255)

                                                                                                               agataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGtt                                       <  2:2248993‑M2/112‑1 (MQ=255)

                                                                                                               agataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGtt                                       >  1:128003‑M2/39‑150 (MQ=255)

                                                                                                                gataattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTa                                      <  2:120540‑M2/113‑1 (MQ=255)

                                                                                                                   aattttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATc                                   >  1:3815351‑M2/35‑150 (MQ=255)

                                                                                                                    attttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCa                                  >  2:2042597‑M2/34‑150 (MQ=255)

                                                                                                                    attttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCa                                  >  2:4003122‑M2/34‑150 (MQ=255)

                                                                                                                     ttttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCag                                 >  2:437978‑M2/33‑150 (MQ=255)

                                                                                                                      tttcgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCaga                                >  1:547234‑M2/32‑150 (MQ=255)

                                                                                                                         cgccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCagaaga                             >  1:2875337‑M2/29‑150 (MQ=255)

                                                                                                                          gccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAgaagaa                            <  1:1004084‑M2/123‑1 (MQ=255)

                                                                                                                          gccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAgaagaa                            >  1:1445735‑M2/28‑150 (MQ=255)

                                                                                                                          gccatgatggagaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAgaagaa                            >  1:2295304‑M2/28‑150 (MQ=255)

                                                                                                                           ccatgatggagaaattaatatgttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGaagaaa                           <  1:984076‑M2/124‑1 (MQ=255)

                                                                                                                                    agaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCTGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTg                  <  1:626129‑M2/133‑1 (MQ=255)

                                                                                                                                    agaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTg                  <  2:4444125‑M2/133‑1 (MQ=255)

                                                                                                                                     gaaattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGc                 >  2:1624150‑M2/17‑150 (MQ=255)

                                                                                                                                       aattaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCtt               <  2:476086‑M2/136‑1 (MQ=255)

                                                                                                                                        attaatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTa              >  1:3438732‑M2/14‑150 (MQ=255)

                                                                                                                                          taatattttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAAc            <  1:3660725‑M2/139‑1 (MQ=255)

                                                                                                                                              attttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGt        <  2:3838059‑M2/143‑1 (MQ=255)

                                                                                                                                              attttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGt        <  2:869604‑M2/143‑1 (MQ=255)

                                                                                                                                               ttttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTa       <  1:3752634‑M2/144‑1 (MQ=255)

                                                                                                                                                tttttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTAt      <  1:354965‑M2/145‑1 (MQ=255)

                                                                                                                                                   ttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTg   >  1:332240‑M2/3‑150 (MQ=255)

                                                                                                                                                   ttCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTg   >  1:209299‑M2/3‑150 (MQ=255)

                                                                                                                                                    tCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGt  >  2:89538‑M2/2‑150 (MQ=255)

CCTGTGTATCTGGGTTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGT  >  AP012306/1325749‑1326046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`