New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_012967 1142669 =111 (0.590)3 (0.020) 3/134 14.1 1.8% coding (259/1536 nt) murJ murein biosynthesis integral membrane protein MurJ
?NC_012967 = 4310192 222 (1.250)coding (977/1503 nt) proP glycine betaine/L‑proline transporter ProP
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 240 total aligned reads displayed.

CGCCATTATGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACG  >  NC_012967/4310121‑4310263
                                                                       |                                                                       
cGCCATTATGATNGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTcc                                                                         <  1:720862/72‑1 (MQ=255)
  ccATTATGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTGCgc                                                                       >  2:2343389/1‑71 (MQ=255)
  ccATTATGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGt                                                                       >  1:3982235/1‑72 (MQ=255)
   cATTATGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGtt                                                                      <  2:48937/72‑1 (MQ=255)
    aTTATGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGttt                                                                     <  2:849903/72‑1 (MQ=255)
    aTTATGATCGGTATGCTGNTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGttt                                                                     <  1:2550904/72‑1 (MQ=255)
    aTTATGATCGGTATCCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGttt                                                                     >  2:494587/1‑72 (MQ=255)
      tgtGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTtgt                                                                   <  1:1089647/70‑1 (MQ=255)
      tATGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTtgt                                                                   <  1:375860/72‑1 (MQ=255)
      tATGATCGGTATGCGGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTtgt                                                                   <  2:2894791/72‑1 (MQ=255)
       aTGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTtgtg                                                                  <  2:1369578/72‑1 (MQ=255)
       aTGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTtgtg                                                                  <  1:3518447/72‑1 (MQ=255)
        tGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGc                                                                 <  2:1196093/72‑1 (MQ=255)
        tGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGc                                                                 <  1:195733/72‑1 (MQ=255)
         gATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTTAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCt                                                                >  1:5814470/1‑72 (MQ=255)
         gATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCAGTTTGTGCt                                                                >  2:3970778/1‑72 (MQ=255)
          ttCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTa                                                               <  1:2130652/71‑1 (MQ=255)
          aTCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTa                                                               <  1:963981/72‑1 (MQ=255)
           tctgTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTAc                                                              >  1:4603909/4‑72 (MQ=255)
            cGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTTCTACt                                                             >  1:5917775/1‑72 (MQ=255)
            cGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACt                                                             <  1:865503/72‑1 (MQ=255)
            cGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACt                                                             <  2:667313/72‑1 (MQ=255)
             ggTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACtt                                                            >  1:2845663/1‑72 (MQ=255)
             ggTATCCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGGGCTCCtt                                                            >  2:4351670/1‑72 (MQ=255)
               tATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTgg                                                          >  1:2471555/1‑72 (MQ=255)
                 tGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTa                                                        <  2:5371171/72‑1 (MQ=255)
                   cTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAgt                                                      <  2:3853209/72‑1 (MQ=255)
                   cTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAgt                                                      <  1:4098041/72‑1 (MQ=255)
                    tttttNTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAgtg                                                     <  2:591029/70‑1 (MQ=255)
                    tGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAgtg                                                     >  1:3194009/1‑72 (MQ=255)
                     gTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGTTAgtgt                                                    >  1:4697457/1‑72 (MQ=255)
                      tttGTCCAGCCGGTGATGGGCTTGCTGAGTNACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGtt                                                   <  2:417321/72‑1 (MQ=255)
                      tttGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGtt                                                   <  2:2035502/72‑1 (MQ=255)
                       ttGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTg                                                  >  1:2679816/1‑72 (MQ=255)
                        tGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGc                                                 <  1:4902391/72‑1 (MQ=255)
                        tGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGc                                                 >  2:6060461/1‑72 (MQ=255)
                        tGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGc                                                 <  2:6227361/72‑1 (MQ=255)
                         gTCCGGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGcc                                                <  2:1012540/72‑1 (MQ=255)
                          tCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGccc                                               <  2:3047831/72‑1 (MQ=255)
                           ccAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCt                                              >  2:5458083/1‑72 (MQ=255)
                           ccAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCt                                              <  1:4837069/72‑1 (MQ=255)
                           ccAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCt                                              <  1:5997016/72‑1 (MQ=255)
                           ccAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCt                                              <  2:2624684/72‑1 (MQ=255)
                            cAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCGCTg                                             >  1:6555887/1‑72 (MQ=255)
                             aGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGt                                            >  2:3209247/1‑72 (MQ=255)
                             aGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGt                                            >  1:1340146/1‑72 (MQ=255)
                                 ggTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTtgt                                        <  2:2092343/72‑1 (MQ=255)
                                 ggTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTtgn                                        >  2:2474702/1‑71 (MQ=255)
                                 ggTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTCGTAGTGTTGCCCTGTTtgt                                        >  1:5532408/1‑72 (MQ=255)
                                  gTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTtgtg                                       <  2:1754100/72‑1 (MQ=255)
                                   tGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTgtgt                                      >  1:1569614/1‑72 (MQ=255)
                                   tGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTgtgt                                      <  1:1134981/72‑1 (MQ=255)
                                    gATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGtt                                     <  2:5190338/72‑1 (MQ=255)
                                     aTGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTg                                    >  2:5119662/1‑72 (MQ=255)
                                     aTGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTg                                    <  1:3987257/72‑1 (MQ=255)
                                      tGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTgg                                   >  1:2632549/1‑72 (MQ=255)
                                      tGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTgg                                   <  2:6397973/72‑1 (MQ=255)
                                      tGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTgg                                   <  1:1933894/72‑1 (MQ=255)
                                        ggCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCg                                 <  2:5249761/72‑1 (MQ=255)
                                        ggCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCg                                 <  1:5405246/72‑1 (MQ=255)
                                         gCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGa                                <  2:3599912/72‑1 (MQ=255)
                                         gCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGa                                >  1:6123360/1‑72 (MQ=255)
                                         gCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGNTGGCgc                                >  2:459433/1‑71 (MQ=255)
                                          cTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGAt                               <  2:6629864/72‑1 (MQ=255)
                                           ttGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGGGagt                              >  2:2836226/1‑70 (MQ=255)
                                           ttGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATc                              <  2:4789142/72‑1 (MQ=255)
                                           ttGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATc                              >  1:6601618/1‑72 (MQ=255)
                                           ttGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATc                              <  1:4340605/72‑1 (MQ=255)
                                            tGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATcc                             >  2:4282621/1‑72 (MQ=255)
                                             gCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGTCGATccc                            <  2:2066625/72‑1 (MQ=255)
                                             gCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATccc                            <  1:5102351/72‑1 (MQ=255)
                                             gCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATccc                            <  2:1600236/72‑1 (MQ=255)
                                              cTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCg                           <  2:6278332/72‑1 (MQ=255)
                                               tgggTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCgg                          <  1:834342/69‑1 (MQ=255)
                                               tGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCACTGTTTGTGTCGGGGATTCCgg                          >  2:881818/1‑72 (MQ=255)
                                                gAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGc                         <  1:3035234/72‑1 (MQ=255)
                                                  gTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGt                       >  1:1003417/1‑72 (MQ=255)
                                                    gACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGttt                     <  2:4490461/72‑1 (MQ=255)
                                                    gACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGgtt                     >  2:2247228/1‑72 (MQ=255)
                                                     tgcGTTTTGGCCGGCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTa                    <  2:5768674/70‑1 (MQ=255)
                                                       cGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTAtt                  <  2:2288062/72‑1 (MQ=255)
                                                       cGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTAtt                  >  1:4661459/1‑72 (MQ=255)
                                                        gTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTTTTc                 >  1:3843102/1‑72 (MQ=255)
                                                        gTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTAttt                 >  1:519187/1‑71 (MQ=255)
                                                        gTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTc                 <  1:6202350/72‑1 (MQ=255)
                                                         ttttGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGGGTTTATTCt                >  1:2940573/1‑72 (MQ=255)
                                                         ttttGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCt                <  1:3120345/72‑1 (MQ=255)
                                                          tttGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTg               <  1:5452045/72‑1 (MQ=255)
                                                           ttGGCCGTCGTCCGTTTGTGCTACTTNGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGa              <  2:713663/72‑1 (MQ=255)
                                                           ttGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGAGTTGGCGATCCCGGCGTTTTTTCttg              >  1:2336400/1‑70 (MQ=255)
                                                             ggCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTTTTTTTAtt            >  2:650963/1‑72 (MQ=255)
                                                             ggCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGAtt            >  1:1397206/1‑72 (MQ=255)
                                                               ccGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTTTGATTaa          >  1:2069302/1‑72 (MQ=255)
                                                                 gtcgtcCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTNGGCGATCCCGGCGTTTATTCTGATTAACa        <  2:559435/72‑1 (MQ=255)
                                                                  tcgtcCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAg       >  2:3274314/1‑72 (MQ=255)
                                                                  tcgtcCGTCTGTGCTACTTGGTAGTGGTGCCCTGTTTGTGTTGTCGGTCCCGGCGTTTATTCTGATTAACAg       <  2:1310144/72‑1 (MQ=255)
                                                                   cgtcCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGt      >  2:6268579/1‑72 (MQ=255)
                                                                    gtcCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTa     >  2:3406012/1‑72 (MQ=255)
                                                                     tcCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTaa    <  1:4135868/72‑1 (MQ=255)
                                                                       cGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACg  <  2:5390757/72‑1 (MQ=255)
                                                                       |                                                                       
CGCCATTATGATCGGTATGCTGTTTGTCCAGCCGGTGATGGGCTTGCTGAGTGACCGTTTTGGCCGTCGTCCGTTTGTGCTACTTGGTAGTGTTGCCCTGTTTGTGTTGGCGATCCCGGCGTTTATTCTGATTAACAGTAACG  >  NC_012967/4310121‑4310263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.