breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR333730_R1.good.fq7,727,786556,365,502100.0%72.0 bases72 bases94.8%
errorsSRR333730_R2.good.fq7,727,785556,365,430100.0%72.0 bases72 bases91.0%
total15,455,5711,112,730,932100.0%72.0 bases72 bases92.9%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionNC_0129674,629,812240.811.5100.0%Escherichia coli B str. REL606, complete sequence.
total4,629,812100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000020193
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000338
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.011

Junction Skew Score Calculation

reference sequencepr(no read start)
NC_0129670.27816

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 3
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 1

Read Alignment Evidence

optionvalue
ModeConsensus/Mixed Base
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff10
Polymorphism frequency cutoff0.2
Polymorphism minimum variant coverage each strandOFF
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs ofOFF
Skip base substitutions when they create a homopolymer flanked on each side byOFF

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input15:22:11 16 Jan 202315:25:35 16 Jan 20233 minutes 24 seconds
Read alignment to reference genome15:25:35 16 Jan 202315:46:44 16 Jan 202321 minutes 9 seconds
Preprocessing alignments for candidate junction identification15:46:44 16 Jan 202315:49:42 16 Jan 20232 minutes 58 seconds
Preliminary analysis of coverage distribution15:49:42 16 Jan 202315:55:35 16 Jan 20235 minutes 53 seconds
Identifying junction candidates15:55:35 16 Jan 202315:55:39 16 Jan 20234 seconds
Re-alignment to junction candidates15:55:39 16 Jan 202315:59:36 16 Jan 20233 minutes 57 seconds
Resolving best read alignments15:59:36 16 Jan 202316:03:49 16 Jan 20234 minutes 13 seconds
Creating BAM files16:03:49 16 Jan 202316:09:15 16 Jan 20235 minutes 26 seconds
Tabulating error counts16:09:15 16 Jan 202316:10:50 16 Jan 20231 minute 35 seconds
Re-calibrating base error rates16:10:50 16 Jan 202316:10:51 16 Jan 20231 second
Examining read alignment evidence16:10:51 16 Jan 202316:27:59 16 Jan 202317 minutes 8 seconds
Polymorphism statistics16:27:59 16 Jan 202316:28:00 16 Jan 20231 second
Output16:28:00 16 Jan 202316:28:28 16 Jan 202328 seconds
Total 1 hour 6 minutes 17 seconds