New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_012967 2527115 =296 (1.180)5 (0.020) 5/128 17.4 1.9% coding (28/651 nt) hyfE hydrogenase 4 membrane subunit
?NC_012967 = 4020488 249 (1.110)coding (123/270 nt) yihD YihD family protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

Only 100 of 284 total aligned reads displayed.

CAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGAT  >  NC_012967/4020417‑4020559
                                                                       |                                                                       
cAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGaa                                                                         <  1:5559763/72‑1 (MQ=255)
cAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGaa                                                                         >  1:8571764/1‑72 (MQ=255)
 aaaaaGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAAc                                                                        >  1:5753961/1‑72 (MQ=255)
 aaaaaGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAAc                                                                        <  2:6899675/72‑1 (MQ=255)
 aaaaaGACCCAGCCCTTAACCGGCTGC‑ATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACt                                                                       <  2:7744088/72‑1 (MQ=255)
  aaaaGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACt                                                                       <  1:3249691/72‑1 (MQ=255)
  aaaaGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACt                                                                       <  2:2966062/72‑1 (MQ=255)
   aaaGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTg                                                                      <  2:7902672/72‑1 (MQ=255)
    aaGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTgg                                                                     >  2:2684417/1‑72 (MQ=255)
     agagCAAGACCTTAACCTGCTGCAATTTTTGCAGAAANNGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGc                                                                    <  1:3348651/72‑1 (MQ=255)
       agCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCgg                                                                  >  2:7774089/1‑72 (MQ=255)
        gCCAGACCTTAACCTGCTGCAATTTTTGCAGAAATTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGa                                                                 >  1:8052974/1‑72 (MQ=255)
          cAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGAtt                                                               >  2:8308284/1‑72 (MQ=255)
          cAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGAtt                                                               <  1:6160707/72‑1 (MQ=255)
          cAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGAtt                                                               >  2:3521645/1‑72 (MQ=255)
             aCCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGa                                                            >  1:2740167/1‑72 (MQ=255)
              ccTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGAc                                                           <  1:8694064/72‑1 (MQ=255)
              ccTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGAc                                                           <  1:3842712/72‑1 (MQ=255)
               cTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACg                                                          <  2:5756228/72‑1 (MQ=255)
                 tAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGTGGATTTGAAggg                                                        >  2:4343061/1‑71 (MQ=255)
                  aaCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGAGGGCGAACTGGCGGATTTGACGGAt                                                       <  1:3576605/72‑1 (MQ=255)
                  aaCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTTGCGGATTTGACGGAt                                                       <  2:4173033/72‑1 (MQ=255)
                  aaCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGAt                                                       <  1:7917126/72‑1 (MQ=255)
                  aaCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGAt                                                       >  1:1472445/1‑72 (MQ=255)
                  aaCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGAt                                                       >  2:7942306/1‑72 (MQ=255)
                   aCCTGCTGCAATTTTTGCAGAAACTGGNGAAAGAGTCAGGTNTTGACGGCGAACTGGCGGATTTGACGGATg                                                      >  1:439622/1‑72 (MQ=255)
                   aCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATg                                                      >  1:8257076/1‑72 (MQ=255)
                   aCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATg                                                      <  2:7181582/72‑1 (MQ=255)
                   aCCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATg                                                      <  1:1903757/72‑1 (MQ=255)
                    ccTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGa                                                     <  2:2510014/72‑1 (MQ=255)
                     ctgctgCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGAc                                                    <  1:4021874/72‑1 (MQ=255)
                        ctgcAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACAtt                                                 >  1:6717316/1‑72 (MQ=255)
                          gcAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCt                                               >  1:4205383/1‑72 (MQ=255)
                          gcAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCt                                               <  2:3835543/72‑1 (MQ=255)
                           cAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTg                                              >  1:3910194/1‑72 (MQ=255)
                           cAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTg                                              >  1:8000050/1‑72 (MQ=255)
                           cAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTg                                              <  1:1624461/72‑1 (MQ=255)
                           cAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTg                                              <  1:1366263/72‑1 (MQ=255)
                           cAATTTGTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTg                                              <  2:3686550/72‑1 (MQ=255)
                            aaTTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTgg                                             <  1:3043642/72‑2 (MQ=255)
                            aaTTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGa                                             <  1:4818828/72‑1 (MQ=255)
                            aaTTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGa                                             >  2:7339004/1‑72 (MQ=255)
                             aTTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGAt                                            <  1:3077538/72‑1 (MQ=255)
                              tttttGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATc                                           <  2:2805234/72‑1 (MQ=255)
                              tttttGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATc                                           <  1:1047544/72‑1 (MQ=255)
                                tttGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTa                                         <  1:5070629/72‑1 (MQ=255)
                                 ttGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTAt                                        <  1:7007697/72‑1 (MQ=255)
                                 ttGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGAGGATTTGACGGATGACATTCTGATCTAt                                        <  1:8347278/72‑1 (MQ=255)
                                  tGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATc                                       <  2:7102680/72‑1 (MQ=255)
                                   gCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACCATCTGATCTATCa                                      >  2:123971/1‑72 (MQ=255)
                                   gCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCa                                      <  2:3198374/72‑1 (MQ=255)
                                   gCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCa                                      <  1:7405991/72‑1 (MQ=255)
                                    cAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCAc                                     <  1:5946818/72‑1 (MQ=255)
                                    aaGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCAc                                     <  2:1046837/71‑1 (MQ=255)
                                      gAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCt                                   <  1:6278685/72‑1 (MQ=255)
                                      gAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCt                                   >  1:6394273/1‑72 (MQ=255)
                                       aaaCTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGAGCTCTCACCtn                                  >  2:3288139/1‑71 (MQ=255)
                                        aaCTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGa                                 >  2:7516813/1‑72 (MQ=255)
                                          cTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGaaa                               >  1:7335355/1‑72 (MQ=255)
                                          cTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGaaa                               <  1:2615708/72‑1 (MQ=255)
                                          cTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGaaa                               <  2:6592066/72‑1 (MQ=255)
                                           tGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGaaaa                              >  2:4442081/1‑72 (MQ=255)
                                           tGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGaaaa                              <  1:4756094/72‑1 (MQ=255)
                                           tGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGaaaa                              >  1:2890090/1‑72 (MQ=255)
                                           tGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGaaaa                              <  2:3457867/72‑1 (MQ=255)
                                           tGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACACTCTGATCTTTCACCTGaaaa                              >  2:3631577/1‑72 (MQ=255)
                                            ggCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATATATCACCTGGAAAt                             >  2:8033603/1‑72 (MQ=255)
                                             gggAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATg                            <  2:218918/70‑1 (MQ=255)
                                             gCGAAAGAGTCAGGTTTTGACGGCGAACTAGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATg                            >  1:1433168/1‑72 (MQ=255)
                                              cGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGGTTTGACGGGTGACATTCTGATCTATCACCTGAAAAAGc                           >  2:838525/1‑72 (MQ=255)
                                              cGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGc                           >  2:1686965/1‑72 (MQ=255)
                                               gAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGct                          >  2:207611/1‑71 (MQ=255)
                                               gAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCg                          >  2:8456024/1‑72 (MQ=255)
                                                  agagTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGTGTGa                       >  2:1903344/1‑72 (MQ=255)
                                                   gagTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGAt                      >  2:5979069/1‑72 (MQ=255)
                                                    agTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGAtt                     >  1:6673270/1‑72 (MQ=255)
                                                      tCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTcc                   >  1:5209263/1‑72 (MQ=255)
                                                       cAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTctg                  >  1:1850396/1‑70 (MQ=255)
                                                       cAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCcg                  >  2:6072143/1‑72 (MQ=255)
                                                       cAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCcg                  >  2:1774767/1‑72 (MQ=255)
                                                        aGGTTTTGACGGCGAACTGGCGGATTTGACTGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCcgc                 <  1:460154/72‑1 (MQ=255)
                                                        aGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCcgc                 <  1:2368018/72‑1 (MQ=255)
                                                        aGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCcgc                 >  2:6289151/1‑72 (MQ=255)
                                                          gTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTTATTCCGCGa               <  2:5916817/72‑1 (MQ=255)
                                                          gTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGa               >  2:4603931/1‑72 (MQ=255)
                                                          gTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGa               >  2:5623495/1‑72 (MQ=255)
                                                             ttGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATGCCGCGAAAg            <  1:3147477/72‑1 (MQ=255)
                                                               gACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCATCTGAAAATGCGTGATTCCGCGAAAGAt          <  2:4886554/72‑1 (MQ=255)
                                                               gACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGAt          <  2:1323142/72‑1 (MQ=255)
                                                               gACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGAt          <  1:7197169/72‑1 (MQ=255)
                                                               gACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGAt          <  2:1654128/72‑1 (MQ=255)
                                                                aCGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATg         >  2:4830697/1‑72 (MQ=255)
                                                                 cGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGc        <  1:6200765/72‑1 (MQ=255)
                                                                 cGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGc        <  2:1878529/72‑1 (MQ=255)
                                                                 cGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGc        <  2:8139426/72‑1 (MQ=255)
                                                                     gAACTGGCGGGTTTGTCGGATGACGTTCTGATCTATCACCTGAAAATGCGTGATTCCGCGGACGCTGCGGTg    >  2:5503275/1‑72 (MQ=255)
                                                                     gAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTg    <  1:1986946/72‑1 (MQ=255)
                                                                       aCTGGCGGATTTTACGGGTGACATTCTGATCTATCACCTGGAAATTCGTGATTCCGCGGAAAATGCGGTGAt  >  2:1138279/1‑72 (MQ=255)
                                                                       aCTGGCGGATTTGACGGGTGACATTCTGATCTATCACCCGAAAAAGCGTGATTTCGCGAAAGATGCGGTggt  >  2:1522325/1‑70 (MQ=255)
                                                                       aCTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGAt  <  2:5065566/72‑1 (MQ=255)
                                                                       |                                                                       
CAAAAAGAGCCAGACCTTAACCTGCTGCAATTTTTGCAGAAACTGGCGAAAGAGTCAGGTTTTGACGGCGAACTGGCGGATTTGACGGATGACATTCTGATCTATCACCTGAAAATGCGTGATTCCGCGAAAGATGCGGTGAT  >  NC_012967/4020417‑4020559

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.