breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 119,184 T→G 11.4% F47V (TTT→GTT)  marB → multiple antibiotic resistance protein MarB
RA NZ_CP022686 162,498 G→T 5.7% G77C (GGT→TGT)  ydgH → DUF1471 family protein YdgH
RA NZ_CP022686 263,831 A→C 10.2% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 277,453 T→G 8.6% H202P (CAC→CCC)  pheS ← phenylalanine‑‑tRNA ligase subunit alpha
RA NZ_CP022686 298,027 A→C 12.3% F60C (TTT→TGT)  chbC ← PTS N,N'‑diacetylchitobiose transporter subunit IIC
RA NZ_CP022686 329,133 C→T 6.8% intergenic (‑81/‑80) ydjA ← / → sppA NAD(P)H nitroreductase/signal peptide peptidase SppA
RA NZ_CP022686 349,106 A→C 11.7% T607P (ACC→CCC)  yeaG → protein kinase YeaG
RA NZ_CP022686 360,227 T→G 21.1% T35P (ACG→CCG)  yeaR ← DUF1971 domain‑containing protein YeaR
RA NZ_CP022686 372,574 A→C 15.6% N275T (AAC→ACC)  sdaA → L‑serine ammonia‑lyase
RA NZ_CP022686 418,374 T→G 8.2% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 487,559 T→G 11.8% T526T (ACA→ACC tar ← methyl‑accepting chemotaxis protein II
RA NZ_CP022686 531,408 A→C 14.0% K44N (AAA→AAC fliF → flagellar basal‑body MS‑ring/collar protein FliF
RA NZ_CP022686 556,942 G→T 12.3% intergenic (+126/‑722) zinT → / → CIW80_RS03080 metal‑binding protein ZinT/site‑specific integrase
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 596,637 T→G 13.3% G54G (GGT→GGG irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,295 T→G 24.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,713 A→C 11.2% R452R (CGT→CGG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,833 A→C 11.7% P243P (CCT→CCG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 651,848 A→C 12.3% R238R (CGT→CGG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 764,997 T→G 15.8% N41T (AAC→ACC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 766,763 A→T 13.3% Y288* (TAT→TAA wcaK ← colanic acid biosynthesis pyruvyl transferase WcaK
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,428 T→G 16.5% intergenic (‑17/+210) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,435 C→A 28.7% intergenic (‑24/+203) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,441 C→A 23.6% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,452 G→T 23.0% intergenic (‑41/+186) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 19.9% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 859,999 T→G 8.0% A620A (GCT→GCG yehM → DUF5682 family protein
RA NZ_CP022686 881,742 A→C 19.4% intergenic (+257/+130) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 24.7% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,054 C→G 7.9% intergenic (+123/‑134) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 12.0% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 13.8% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 1,000,431 T→G 30.8% D945A (GAC→GCC)  yfaL ← AIDA‑I family autotransporter adhesin YfaL/EhaC
RA NZ_CP022686 1,005,085 C→A 14.2% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,011,426 G→C 12.8% M1M (ATG→ATC) † glpA → anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
RA NZ_CP022686 1,064,076 A→C 6.2% T161P (ACG→CCG)  yfbR → 5'‑deoxynucleotidase
RA NZ_CP022686 1,065,974 G→C 9.3% A38G (GCA→GGA)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,126,401 A→C 8.1% A532A (GCT→GCG CIW80_RS05650 ← phase‑variable autotransporter adhesin UpaE
RA NZ_CP022686 1,137,229 A→C 7.1% R243R (CGT→CGG emrK ← multidrug efflux MFS transporter periplasmic adaptor subunit EmrK
RA NZ_CP022686 1,174,272 A→C 14.6% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,174,416 A→T 7.4% intergenic (+26/‑21) CIW80_RS05860 → / → CIW80_RS05865 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,197,189 T→G 5.1% V90G (GTG→GGG)  murQ → N‑acetylmuramic acid 6‑phosphate etherase
RA NZ_CP022686 1,272,651 T→G 5.6% N91T (AAC→ACC)  CIW80_RS06320 ← DUF823 domain‑containing adhesin
RA NZ_CP022686 1,360,709 T→G 11.5% intergenic (‑44/+280) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,724 G→T 9.0% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,756 C→A 9.3% intergenic (‑91/+233) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,783 G→T 7.8% intergenic (‑118/+206) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 13.8% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,370,559 T→G 11.8% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,396,420 T→G 7.4% intergenic (+26/‑211) gabT → / → gabP 4‑aminobutyrate‑‑2‑oxoglutarate transaminase/GABA permease
RA NZ_CP022686 1,479,607 A→C 13.2% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,619 T→A 10.3% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,305 A→T 9.4% N248Y (AAT→TAT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,339 C→A 19.7% P259Q (CCA→CAA)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,351 A→C 12.0% N263T (AAC→ACC)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 11.1% A264A (GCT→GCA CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,000 G→C 8.2% intergenic (+22/‑12) CIW80_RS07705 → / → CIW80_RS07710 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 24.1% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,558,395 A→C 13.0% K464N (AAA→AAC vgrG → type VI secretion system Vgr family protein
RA NZ_CP022686 1,564,543 A→C 11.2% S182R (AGC→CGC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,480 T→G 7.3% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,632,501 T→G 7.1% V166G (GTT→GGT)  arcC → carbamate kinase
RA NZ_CP022686 1,633,846 T→G 29.1% N253T (AAC→ACC)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,644,964 T→G 6.8% G83G (GGT→GGG xanQ → xanthine/proton symporter XanQ
RA NZ_CP022686 1,674,244 T→G 15.4% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 12.3% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,705,974 T→G 15.8% intergenic (‑584/‑71) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,706,025 C→A 22.1% intergenic (‑635/‑20) yqgB ← / → metK acid stress response protein YqgB/methionine adenosyltransferase
RA NZ_CP022686 1,716,145 A→C 9.8% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,716,167 A→C 11.0% N49T (AAC→ACC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,828,113 A→C 9.6% P84P (CCT→CCG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,832,023 T→G 8.0% N355T (AAC→ACC)  gspE ← type II secretion system ATPase GspE
RA NZ_CP022686 1,925,332 A→C 8.7% N151T (AAC→ACC)  ygiB → DUF1190 family protein
RA NZ_CP022686 2,016,175 T→G 8.9% noncoding (99/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,016,225 T→G 8.0% noncoding (49/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,037,521 A→C 11.6% T508P (ACC→CCC)  lpoA → penicillin‑binding protein activator LpoA
RA NZ_CP022686 2,061,804 A→C 10.9% K391N (AAA→AAC argG → argininosuccinate synthase
RA NZ_CP022686 2,110,457 T→G 8.6% K12N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,465 T→G 6.9% T10P (ACC→CCC)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,110,472 T→G 7.4% K7N (AAA→AAC rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,130,829 A→C 8.2% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,115 T→G 10.4% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,122 T→G 7.9% S128R (AGT→AGG panF → sodium/pantothenate symporter
RA NZ_CP022686 2,148,139 C→G 10.5% A134G (GCG→GGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,150,098 T→G 8.0% intergenic (+15/‑314) prmA → / → dusB 50S ribosomal protein L11 methyltransferase/tRNA dihydrouridine synthase DusB
RA NZ_CP022686 2,185,075 A→C 6.1% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,192,062 A→C 6.8% R69R (CGT→CGG rplD ← 50S ribosomal protein L4
RA NZ_CP022686 2,194,054 G→T 7.9% Q444K (CAA→AAA)  gspA ← type II secretion system protein GspA
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,272,790 C→G 9.1% intergenic (+32/‑65) greB → / → yhgF transcription elongation factor GreB/Tex family protein
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,393,013 T→G 7.3% intergenic (+151/‑2) slp → / → dctR outer membrane lipoprotein Slp/LuxR C‑terminal‑related transcriptional regulator
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,448,912 G→T 7.5% A180S (GCG→TCG)  yhjV → aromatic amino acid transport family protein
RA NZ_CP022686 2,460,514 G→C 6.4% A22G (GCC→GGC)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,576 C→A 11.6% M1M (ATG→ATT) † yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,581 A→C 16.3% intergenic (‑3/‑155) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,516,873 A→C 10.3% K761N (AAA→AAC upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 13.8% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 9.8% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,408 C→A 11.4% M90I (ATG→ATT waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,559,398 T→G 7.2% N461T (AAC→ACC)  ligB ← NAD‑dependent DNA ligase LigB
RA NZ_CP022686 2,572,705 A→C 10.6% G191G (GGT→GGG CIW80_RS12885 ← ROK family protein
RA NZ_CP022686 2,574,429 T→G 13.0% N230T (AAC→ACC)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
RA NZ_CP022686 2,574,451 T→G 14.0% T223P (ACT→CCT)  CIW80_RS12895 ← class II fructose‑bisphosphate aldolase family protein
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,717,385 T→G 9.8% F96V (TTT→GTT)  ilvA → threonine ammonia‑lyase, biosynthetic
RA NZ_CP022686 2,736,578 C→A 8.3% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,585 T→A 7.2% Y183N (TAC→AAC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 13.1% Y183S (TAC→TCC) ‡ rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,754,985 C→G 7.5% M1M (ATG→ATC) † cyaY ← iron donor protein CyaY
RA NZ_CP022686 2,758,109 A→C 6.8% N34T (AAC→ACC)  yifL → lipoprotein
RA NZ_CP022686 2,758,113 A→C 7.2% A35A (GCA→GCC yifL → lipoprotein
RA NZ_CP022686 2,758,128 A→C 10.8% K40N (AAA→AAC yifL → lipoprotein
RA NZ_CP022686 2,758,137 G→C 7.7% E43D (GAG→GAC yifL → lipoprotein
RA NZ_CP022686 2,758,144 A→C 10.0% T46P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,153 A→C 8.0% T49P (ACG→CCG)  yifL → lipoprotein
RA NZ_CP022686 2,758,168 A→C 7.2% N54H (AAC→CAC)  yifL → lipoprotein
RA NZ_CP022686 2,801,817 T→G 6.1% Y32S (TAC→TCC)  CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,801,864 T→G 7.4% L16F (TTA→TTC CIW80_RS14030 ← 2‑dehydro‑3‑deoxy‑6‑phosphogalactonate aldolase
RA NZ_CP022686 2,834,886 A→C 9.3% K597N (AAA→AAC polA → DNA polymerase I
RA NZ_CP022686 2,868,740 T→G 23.6% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,745 C→G 7.0% A20A (GCG→GCC fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 8.0% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 9.5% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,920,428 A→C 10.6% K68N (AAA→AAC CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,981,612 A→C 8.0% T567P (ACC→CCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,981,625 A→C 6.8% D571A (GAC→GCC)  rpoC → DNA‑directed RNA polymerase subunit beta'
RA NZ_CP022686 2,989,627 T→G 9.4% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,020,229 T→G 15.9% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,080,461 T→G 7.0% R158R (CGT→CGG CIW80_RS15320 → Ldh family oxidoreductase
RA NZ_CP022686 3,098,847 A→C 8.0% intergenic (+17/+22) CIW80_RS15415 → / ← actP LrgB family protein/cation/acetate symporter ActP
RA NZ_CP022686 3,136,940 T→G 9.5% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,184,651 A→C 11.1% intergenic (‑98/+18) cutA ← / ← dcuA divalent cation tolerance protein CutA/anaerobic C4‑dicarboxylate transporter DcuA
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,252,190 T→G 12.6% K221N (AAA→AAC CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,265,121 G→T 6.7% Q65K (CAA→AAA)  ytfJ ← YtfJ family protein
RA NZ_CP022686 3,297,333 T→G 10.0% F828V (TTT→GTT)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,334 T→G 6.0% intergenic (‑150/‑50) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,342 A→T 11.2% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,357 T→G 7.3% intergenic (‑173/‑27) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,357,446 C→A 6.6% T10K (ACG→AAG)  CIW80_RS16765 → DUF932 domain‑containing protein
RA NZ_CP022686 3,387,644 A→C 12.3% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,625 C→A 12.0% D140Y (GAT→TAT)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,411,341 T→G 5.5% V114G (GTT→GGT)  fimH → type 1 fimbria D‑mannose specific adhesin FimH
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,444,785 T→G 5.3% T503P (ACC→CCC)  btsT ← pyruvate/proton symporter BtsT
RA NZ_CP022686 3,461,229 T→G 18.7% noncoding (84/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 9.5% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,461,363 A→C 14.6% noncoding (65/87 nt) CIW80_RS17345 ← tRNA‑Leu
JC NZ_CP022686 3,509,100 Δ12 bp 45.1% coding (1567‑1578/1917 nt) dnaK → molecular chaperone DnaK
RA NZ_CP022686 3,583,665 A→C 6.4% Q342H (CAA→CAC ilvI → acetolactate synthase 3 large subunit
RA NZ_CP022686 3,625,683 T→G 10.8% A150A (GCT→GCG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,625,704 T→G 7.8% R157R (CGT→CGG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,634,614 A→C 7.3% K267N (AAA→AAC acnB → bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
RA NZ_CP022686 3,652,079 T→G 9.3% intergenic (‑88/+24) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,652,090 T→G 8.1% intergenic (‑99/+13) panB ← / ← CIW80_RS18255 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase/fimbrial‑like adhesin
RA NZ_CP022686 3,655,540 A→C 7.6% F765V (TTT→GTT)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,660,810 C→A 11.2% R245L (CGC→CTC)  pcnB ← polynucleotide adenylyltransferase PcnB
RA NZ_CP022686 3,665,907 A→C 5.1% Q462H (CAA→CAC hrpB → ATP‑dependent helicase HrpB
RA NZ_CP022686 3,665,943 A→C 8.9% K474N (AAA→AAC hrpB → ATP‑dependent helicase HrpB
RA NZ_CP022686 3,703,648 A→C 9.4% T120P (ACG→CCG)  fabZ → 3‑hydroxyacyl‑ACP dehydratase FabZ
RA NZ_CP022686 3,731,466 T→C 100% intergenic (+20/‑35) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,470 C→T 100% intergenic (+24/‑31) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,731,473 Δ1 bp 100% intergenic (+27/‑28) rrf → / → CIW80_RS18620 5S ribosomal RNA/tRNA‑Asp
RA NZ_CP022686 3,734,367 T→G 22.5% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,734,380 T→G 19.8% L229R (CTC→CGC)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,481 T→G 16.4% L130V (TTA→GTA)  CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,798,080 T→G 7.3% R329R (CGT→CGG CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,826,780 A→C 5.0% E638D (GAA→GAC CIW80_RS19160 → type I secretion system permease/ATPase
RA NZ_CP022686 3,852,459 T→G 10.0% N178T (AAC→ACC)  phoE ← phosphoporin PhoE
RA NZ_CP022686 3,868,846 T→G 10.3% T383P (ACG→CCG)  ecpC ← fimbrial usher EcpC
RA NZ_CP022686 3,885,457 A→C 13.0% N1404T (AAC→ACC)  fdeC → inverse autotransporter adhesin FdeC
RA NZ_CP022686 3,885,499 A→C 8.7% intergenic (+2/+123) fdeC → / ← CIW80_RS19455 inverse autotransporter adhesin FdeC/helix‑turn‑helix domain‑containing protein
RA NZ_CP022686 3,925,289 A→C 13.4% N156T (AAC→ACC)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,304 A→C 12.3% Q161P (CAG→CCG)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,925,309 G→T 12.6% E163* (GAA→TAA)  prpC → 2‑methylcitrate synthase
RA NZ_CP022686 3,928,369 T→G 12.8% W285G (TGG→GGG)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,928,376 T→G 9.5% V287G (GTA→GGA)  prpE → propionate‑‑CoA ligase
RA NZ_CP022686 3,935,324 T→G 11.2% N705T (AAC→ACC)  lacZ ← beta‑galactosidase
RA NZ_CP022686 3,940,707 A→C 11.3% F314V (TTT→GTT)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,942,556 T→G 6.1% T121P (ACG→CCG)  yaiO ← YaiO family outer membrane beta‑barrel protein
RA NZ_CP022686 3,981,933 T→G 14.6% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 9.5% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,018,211 T→G 6.2% intergenic (+146/‑42) clpX → / → lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
RA NZ_CP022686 4,032,292 A→C 16.0% E106D (GAA→GAC glnK → P‑II family nitrogen regulator
RA NZ_CP022686 4,056,962 C→A 13.8% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,079,329 A→C 6.0% S170A (TCA→GCA)  ybbO ← NADP(+)‑dependent aldehyde reductase
RA NZ_CP022686 4,133,839 T→G 11.6% K117T (AAA→ACA)  ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,196,276 T→G 16.1% E15D (GAA→GAC holA ← DNA polymerase III subunit delta
RA NZ_CP022686 4,249,121 A→C 10.2% V35G (GTC→GGC)  kdpD ← two‑component system sensor histidine kinase KdpD
RA NZ_CP022686 4,368,341 T→G 16.0% D47A (GAC→GCC)  ybiY ← glycyl‑radical enzyme activating protein
RA NZ_CP022686 4,485,614 T→G 10.6% F139V (TTT→GTT)  gloC → hydroxyacylglutathione hydrolase GloC
RA NZ_CP022686 4,547,558 A→C 10.4% T144P (ACC→CCC)  torD → molecular chaperone TorD
RA NZ_CP022686 4,571,439 T→G 9.3% H59P (CAC→CCC)  pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,583,784 G→C 8.4% R78S (AGG→AGC CIW80_RS22900 → YfjI family protein
RA NZ_CP022686 4,587,091 T→G 11.9% N609T (AAC→ACC)  CIW80_RS22915 ← enterotoxin
RA NZ_CP022686 4,632,182 T→G 10.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,678,047 A→C 34.9% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,712,763 A→C 16.4% A52A (GCT→GCG yceB ← lipoprotein
RA NZ_CP022686 4,764,880 T→G 12.6% intergenic (‑60/‑201) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,780,176 A→C 17.4% intergenic (‑75/+95) phoP ← / ← purB two‑component system response regulator PhoP/adenylosuccinate lyase
RA NZ_CP022686 4,809,441 T→G 11.3% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,990 T→G 10.3% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,886 T→G 10.5% V25G (GTG→GGG)  CIW80_RS24305 → C40 family peptidase
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,910,213 A→C 5.4% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 4,947,296 T→G 12.8% V29G (GTG→GGG)  rluB → 23S rRNA pseudouridine(2605) synthase RluB
RA NZ_CP022686 4,976,169 A→C 8.3% N826T (AAC→ACC)  CIW80_RS25180 → efflux RND transporter permease subunit
RA NZ_CP022686 5,001,164 A→C 21.8% N163T (AAC→ACC)  ompG → monomeric porin OmpG
RA NZ_CP022686 5,042,304 T→C 11.0% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,558 T→C 6.1% S1063S (AGT→AGC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,648 G→C 7.3% V1093V (GTG→GTC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,855 T→A 13.6% G1162G (GGT→GGA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,641 C→T 5.3% N1424N (AAC→AAT CIW80_RS25525 → EntS/YbdA MFS transporter
RA pMut‑Ompa‑D5‑IL2wt‑His6 2,031 G→A 100% intergenic (‑235/‑1500) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,465 +GGCTAGCTCA 13.1% intergenic (‑1669/‑66) putative rom (RNA I inhibition modulator protein) ← / → ompA –/–
JC pMut‑Ompa‑D5‑IL2wt‑His6 3,595 Δ15 bp 100% [IL2wt no signal] [IL2wt no signal]
JC pMut‑Ompa‑D5‑IL2wt‑His6 4,019 +CAT 7.8% intergenic (+12/+37) IL2wt no signal → / ← annotated as hok_sok locus –/–

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 188–2618 3086–2957 340–2899 8957 [8714] [8827] 9052 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pMut‑Ompa‑D5‑IL2wt‑His6 4746–4889 5478–5074 186–733 4090 [3947] [0] NA [putative HTH domain protein] [putative HTH domain protein]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 = 1524130NA (NA)43 (0.010) 30/250 NT 5.2% intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
?pMut‑Ompa‑D5‑IL2wt‑His6 3633 = 791 (0.120)coding (25/399 nt) IL2wt no signal
* ? NZ_CP022686 = 1761697NA (NA)96 (0.030) 50/250 NT 10.8% intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 3633 = 791 (0.120)coding (25/399 nt) IL2wt no signal
* ? NZ_CP022686 = 1761699NA (NA)22 (0.010)
+24 bp
16/202 NT 77.3% intergenic (‑74/‑68) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 8 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3393988 =NA (NA)85 (0.030)
+23 bp
41/204 NT 92.9% intergenic (‑51/+11) CIW80_RS16975/CIW80_RS16980 hypothetical protein/IS3‑like element ISEc52 family transposase
?pMut‑Ompa‑D5‑IL2wt‑His6 = 3594 8 (0.000)intergenic (+1/‑15) ompA/IL2wt no signal –/–
* ? NZ_CP022686 3899666 =204 (0.980)23 (0.130) 18/218 NT 11.2% intergenic (‑40/+290) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 3899944 = 186 (1.020)intergenic (‑318/+12) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
* ? NZ_CP022686 = 3899681195 (0.930)34 (0.190) 22/218 NT 16.0% intergenic (‑55/+275) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA
?NZ_CP022686 = 3899927 186 (1.020)intergenic (‑301/+29) hyxR/ipbA LuxR family transcriptional regulator HyxR/tyrosine‑type DNA invertase IpbA