breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence seq id position mutation freq annotation gene description
RA NZ_CP022686 182,646 A→C 11.0% G68G (GGT→GGG malI ← mal regulon transcriptional regulator MalI
RA NZ_CP022686 192,380 T→A 9.4% R597R (CGT→CGA rsxC → electron transport complex subunit RsxC
RA NZ_CP022686 263,831 A→C 7.2% intergenic (+38/+19) fadK → / ← ppsA medium‑chain fatty‑acid‑‑CoA ligase/phosphoenolpyruvate synthase
RA NZ_CP022686 418,374 T→G 11.0% F48V (TTT→GTT)  znuB → zinc ABC transporter permease subunit ZnuB
RA NZ_CP022686 457,894 Δ1 bp 100% pseudogene (288/997 nt) CIW80_RS02445 ← DUF968 domain‑containing protein
RA NZ_CP022686 496,086 A→C 11.5% L399V (TTA→GTA)  otsA ← alpha,alpha‑trehalose‑phosphate synthase
RA NZ_CP022686 524,828 T→G 9.5% R198R (CGT→CGG yedE → selenium metabolism membrane protein YedE/FdhT
RA NZ_CP022686 560,880 C→T 100% intergenic (+62/+53) CIW80_RS03085 → / ← pilV hypothetical protein/shufflon system plasmid conjugative transfer pilus tip adhesin PilV
RA NZ_CP022686 601,295 T→G 17.5% V1607G (GTC→GGC)  irp1 → yersiniabactin polyketide synthase HMWP1
RA NZ_CP022686 601,302 T→G 20.9% R1609R (CGT→CGG irp1 → yersiniabactin polyketide synthase HMWP1
MC JC NZ_CP022686 628,478 Δ1,286 bp 100% [clbP] [clbP]
RA NZ_CP022686 636,713 A→C 11.1% R452R (CGT→CGG clbM ← precolibactin export MATE transporter ClbM
RA NZ_CP022686 651,833 A→C 9.4% P243P (CCT→CCG clbJ ← colibactin non‑ribosomal peptide synthetase ClbJ
RA NZ_CP022686 756,115 A→G 100% G181G (GGT→GGC galE ← UDP‑glucose 4‑epimerase GalE
RA NZ_CP022686 765,001 A→T 12.2% Y40N (TAC→AAC)  wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 765,014 A→T 11.9% V35V (GTT→GTA wcaM ← colanic acid biosynthesis protein WcaM
RA NZ_CP022686 794,460 C→T 100% A568V (GCG→GTG)  dgcE → diguanylate cyclase
RA NZ_CP022686 829,441 C→A 25.2% intergenic (‑30/+197) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 829,467 C→A 21.4% intergenic (‑56/+171) gatY ← / ← fbaB tagatose‑bisphosphate aldolase subunit GatY/class I fructose‑bisphosphate aldolase
RA NZ_CP022686 857,274 T→G 8.8% G78G (GGT→GGG yehL → MoxR family ATPase
RA NZ_CP022686 881,739 A→C 19.4% intergenic (+254/+133) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 881,760 T→G 18.4% intergenic (+275/+112) yohP → / ← dusC protein YohP/tRNA dihydrouridine(16) synthase DusC
RA NZ_CP022686 917,427:1 +TT 100% intergenic (+130/‑91) yeiP → / → yeiQ elongation factor P‑like protein YeiP/mannitol dehydrogenase family protein
RA NZ_CP022686 948,027 A→T 11.2% intergenic (+96/‑161) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,080 G→T 13.2% intergenic (+149/‑108) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,090 C→A 20.9% intergenic (+159/‑98) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 948,105 A→C 12.8% intergenic (+174/‑83) CIW80_RS04855 → / → CIW80_RS04860 terminase small subunit/hypothetical protein
RA NZ_CP022686 957,112 T→G 11.1% T89P (ACC→CCC)  ccmA ← cytochrome c biogenesis heme‑transporting ATPase CcmA
RA NZ_CP022686 1,005,085 C→A 11.9% T380T (ACC→ACA nrdA → ribonucleoside‑diphosphate reductase subunit alpha
RA NZ_CP022686 1,040,909 A→C 11.4% E232D (GAA→GAC rbn → ribonuclease BN
RA NZ_CP022686 1,065,984 C→A 10.2% V35F (GTC→TTC)  yfbS ← SLC13 family permease
RA NZ_CP022686 1,065,997 A→C 14.0% A30A (GCT→GCG yfbS ← SLC13 family permease
RA NZ_CP022686 1,100,959 T→G 14.6% D182A (GAC→GCC)  aroC ← chorismate synthase
RA NZ_CP022686 1,174,272 A→C 13.5% intergenic (+1/‑43) CIW80_RS05855 → / → CIW80_RS05860 tRNA‑Val/tRNA‑Val
RA NZ_CP022686 1,237,935 A→C 12.3% G297G (GGT→GGG tmcA ← tRNA cytosine(34) acetyltransferase TmcA
RA NZ_CP022686 1,360,724 G→T 9.8% intergenic (‑59/+265) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,360,736 G→T 10.3% intergenic (‑71/+253) kgtP ← / ← rrf alpha‑ketoglutarate permease/5S ribosomal RNA
RA NZ_CP022686 1,370,556 T→G 14.6% P99P (CCA→CCC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,370,559 T→G 12.3% K98N (AAA→AAC rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase RluD
RA NZ_CP022686 1,460,615 A→C 13.0% K673N (AAA→AAC flhA → formate hydrogenlyase transcriptional activator FlhA
RA NZ_CP022686 1,479,607 A→C 12.4% intergenic (‑16/+177) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,479,619 T→A 9.6% intergenic (‑28/+165) ftsB ← / ← ygbE cell division protein FtsB/DUF3561 family protein
RA NZ_CP022686 1,504,257 A→T 14.3% S232C (AGT→TGT)  CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,289 T→G 9.3% R242R (CGT→CGG CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,310 G→T 9.6% M249I (ATG→ATT CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,354 C→A 11.7% A264D (GCT→GAT) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,504,355 T→A 9.8% A264A (GCT→GCA) ‡ CIW80_RS07520 → YgcG family protein
RA NZ_CP022686 1,547,096 T→A 12.4% intergenic (+8/‑26) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,547,110 G→C 20.9% intergenic (+22/‑12) CIW80_RS07710 → / → CIW80_RS07715 tRNA‑Met/tRNA‑Met
RA NZ_CP022686 1,564,569 A→C 10.8% K190N (AAA→AAC CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,564,589 A→C 11.8% N197T (AAC→ACC)  CIW80_RS07780 → ImcF‑related family protein
RA NZ_CP022686 1,632,480 T→G 7.9% V159G (GTC→GGC)  arcC → carbamate kinase
RA NZ_CP022686 1,633,901 T→G 8.5% T235P (ACG→CCG)  yqeB ← selenium‑dependent molybdenum cofactor biosynthesis protein YqeB
RA NZ_CP022686 1,674,224 T→G 19.5% K188N (AAA→AAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,244 T→G 16.9% T182P (ACC→CCC)  ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,674,248 T→G 14.7% Q180H (CAA→CAC ygfB ← UPF0149 family protein YgfB
RA NZ_CP022686 1,716,145 A→C 10.4% T42P (ACC→CCC)  rdgB → XTP/dITP diphosphatase
RA NZ_CP022686 1,828,113 A→C 8.9% P84P (CCT→CCG gspK ← type II secretion system minor pseudopilin GspK
RA NZ_CP022686 1,860,077 G→T 13.3% G116G (GGC→GGA CIW80_RS09255 ← YtfJ family protein
RA NZ_CP022686 1,872,518 A→C 5.1% G231G (GGT→GGG hybC ← hydrogenase 2 large subunit
RA NZ_CP022686 1,874,272 A→C 6.3% G38G (GGT→GGG hybB ← Ni/Fe‑hydrogenase cytochrome b subunit
RA NZ_CP022686 1,911,602 T→G 11.7% N224T (AAC→ACC)  CIW80_RS09520 ← MurR/RpiR family transcriptional regulator
RA NZ_CP022686 1,954,923 T→G 19.1% T92P (ACG→CCG)  ttdR ← DNA‑binding transcriptional activator TtdR
RA NZ_CP022686 2,016,170 A→C 5.8% noncoding (104/377 nt) rnpB ← RNase P RNA component class A
RA NZ_CP022686 2,027,390 A→C 7.2% K80N (AAA→AAC agaE → PTS N‑acetylgalactosamine transporter subunit IID
RA NZ_CP022686 2,049,089 T→G 13.4% N256T (AAC→ACC)  deaD ← ATP‑dependent RNA helicase DeaD
RA NZ_CP022686 2,110,462 C→A 7.8% V11L (GTA→TTA)  rplM ← 50S ribosomal protein L13
RA NZ_CP022686 2,123,241 A→C 7.3% F208V (TTT→GTT)  tldD ← metalloprotease TldD
RA NZ_CP022686 2,130,829 A→C 8.8% V237G (GTT→GGT)  mreC ← rod shape‑determining protein MreC
RA NZ_CP022686 2,148,115 T→G 14.8% L126R (CTG→CGG)  panF → sodium/pantothenate symporter
RA NZ_CP022686 2,159,652 A→C 10.7% N160T (AAC→ACC)  CIW80_RS10780 → amino acid ABC transporter substrate‑binding protein
RA NZ_CP022686 2,172,177 A→C 6.4% G87G (GGT→GGG smg ← DUF494 family protein Smg
RA NZ_CP022686 2,179,878 T→G 9.2% K35T (AAG→ACG)  rplQ ← 50S ribosomal protein L17
RA NZ_CP022686 2,185,075 A→C 11.3% V46G (GTT→GGT)  rpsE ← 30S ribosomal protein S5
RA NZ_CP022686 2,214,419 T→C 100% K43R (AAA→AGA)  rpsL ← 30S ribosomal protein S12
RA NZ_CP022686 2,226,139 A→C 6.7% D9A (GAC→GCC)  crp → cAMP‑activated global transcriptional regulator CRP
RA NZ_CP022686 2,320,775 C→T 100% G274S (GGC→AGC)  CIW80_RS11675 ← DNA‑processing protein DprA
RA NZ_CP022686 2,353,173 T→G 5.8% L108V (TTG→GTG)  yhhN → lysoplasmalogenase
RA NZ_CP022686 2,386,496 T→G 9.6% K417N (AAA→AAC prlC ← oligopeptidase A
RA NZ_CP022686 2,394,287 T→G 16.2% N115T (AAC→ACC)  chuS ← hematinate‑forming heme oxygenase ChuS
RA NZ_CP022686 2,408,861 Δ1 bp 100% pseudogene (1097/3115 nt) mdtF → multidrug efflux pump RND permease MdtF
RA NZ_CP022686 2,460,526 A→C 9.4% V18G (GTG→GGG)  yhjY ← autotransporter outer membrane beta‑barrel domain‑containing protein
RA NZ_CP022686 2,460,579 T→A 17.7% intergenic (‑1/‑157) yhjY ← / → tag autotransporter outer membrane beta‑barrel domain‑containing protein/DNA‑3‑methyladenine glycosylase I
RA NZ_CP022686 2,470,422 T→G 13.8% K72N (AAA→AAC glyS ← glycine‑‑tRNA ligase subunit beta
RA NZ_CP022686 2,474,839 A→C 6.6% V258G (GTC→GGC)  xylB ← xylulokinase
RA NZ_CP022686 2,516,872 A→C 7.2% K761T (AAA→ACA)  upaG → trimeric autotransporter adhesin UpaG
RA NZ_CP022686 2,546,394 A→C 10.9% V95G (GTT→GGT)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,546,400 A→C 12.2% L93R (CTG→CGG)  waaG ← glycosyltransferase family 4 protein
RA NZ_CP022686 2,550,305 T→G 8.2% K151N (AAA→AAC mutM ← bifunctional DNA‑formamidopyrimidine glycosylase/DNA‑(apurinic or apyrimidinic site) lyase
RA NZ_CP022686 2,555,399 T→A 13.8% intergenic (‑26/+40) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH
JC NZ_CP022686 2,590,563 +TTGG 100% coding (109/153 nt) CIW80_RS26455 → hypothetical protein
RA NZ_CP022686 2,638,855 C→A 11.9% intergenic (‑170/+70) yidB ← / ← gyrB YidB family protein/DNA topoisomerase (ATP‑hydrolyzing) subunit B
RA NZ_CP022686 2,677,673 T→G 6.0% E73D (GAA→GAC atpD ← F0F1 ATP synthase subunit beta
RA NZ_CP022686 2,736,578 C→A 7.6% T180T (ACC→ACA rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,736,586 A→C 8.0% Y183S (TAC→TCC)  rffA → dTDP‑4‑amino‑4,6‑dideoxygalactose transaminase
RA NZ_CP022686 2,769,179 G→C 12.0% L53V (CTG→GTG)  rarD ← EamA family transporter RarD
RA NZ_CP022686 2,868,740 T→G 21.8% Q22P (CAG→CCG)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,868,753 T→G 14.3% T18P (ACC→CCC)  fdoH ← formate dehydrogenase O subunit beta
RA NZ_CP022686 2,871,422 T→G 9.8% N149T (AAC→ACC)  fdnG ← formate dehydrogenase‑N subunit alpha
RA NZ_CP022686 2,904,852 T→G 11.1% T239P (ACG→CCG)  ftsN ← cell division protein FtsN
RA NZ_CP022686 2,904,856 T→G 6.6% K237N (AAA→AAC ftsN ← cell division protein FtsN
RA NZ_CP022686 2,908,932 A→C 7.2% F36V (TTT→GTT)  priA ← primosomal protein N'
RA NZ_CP022686 2,920,404 T→A 6.3% V60V (GTT→GTA CIW80_RS14610 → bifunctional UDP‑sugar hydrolase/5'‑nucleotidase
RA NZ_CP022686 2,928,566 G→T 5.8% Q6K (CAA→AAA)  gldA ← bifunctional L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
RA NZ_CP022686 2,989,627 T→G 11.8% Q39H (CAA→CAC thiC ← phosphomethylpyrimidine synthase ThiC
RA NZ_CP022686 3,017,622 A→C 11.1% K839N (AAA→AAC metH → methionine synthase
RA NZ_CP022686 3,020,229 T→G 13.8% V133G (GTC→GGC)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,020,247 T→G 5.9% L139R (CTG→CGG)  yjbB → Na/Pi cotransporter family protein
RA NZ_CP022686 3,070,955 C→A 17.4% S43* (TCA→TAA)  CIW80_RS15290 → 2‑oxoglutarate dehydrogenase E1 component
RA NZ_CP022686 3,106,485 T→G 6.3% G67G (GGT→GGG nrfD → cytochrome c nitrite reductase subunit NrfD
RA NZ_CP022686 3,114,653 A→C 6.3% V36G (GTC→GGC)  CIW80_RS15500 ← ABC transporter ATP‑binding protein
RA NZ_CP022686 3,136,940 T→G 11.8% D161A (GAC→GCC)  phnN ← ribose 1,5‑bisphosphokinase
RA NZ_CP022686 3,150,241 T→G 7.0% V16G (GTT→GGT)  rdcB → YjcZ‑like family protein
RA NZ_CP022686 3,150,950 A→C 6.8% Q252H (CAA→CAC rdcB → YjcZ‑like family protein
RA NZ_CP022686 3,151,793 G→T 5.6% A153S (GCG→TCG)  proP → glycine betaine/L‑proline transporter ProP
JC NZ_CP022686 3,196,873 +GCGTAATCGGTT 100% coding (944/1122 nt) ampC ← BlaEC family class C beta‑lactamase
RA NZ_CP022686 3,252,198 C→A 13.5% E219* (GAA→TAA)  CIW80_RS16225 ← enoyl‑CoA hydratase/isomerase family protein
RA NZ_CP022686 3,281,062 T→G 11.1% V94G (GTG→GGG)  yjfF → sugar ABC transporter permease YjfF
RA NZ_CP022686 3,297,360 T→G 6.4% L837V (TTA→GTA)  mgtA → magnesium‑translocating P‑type ATPase
RA NZ_CP022686 3,348,342 A→T 6.7% intergenic (‑158/‑42) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,345 G→T 9.2% intergenic (‑161/‑39) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,348,371 T→G 8.7% intergenic (‑187/‑13) CIW80_RS16685 ← / → CIW80_RS26495 inovirus Gp2 family protein/hypothetical protein
RA NZ_CP022686 3,387,644 A→C 12.1% L222R (CTG→CGG)  sgcQ ← BtpA family protein SgcQ
RA NZ_CP022686 3,390,622 T→A 8.0% I141F (ATT→TTT)  sgcX ← M42 family metallopeptidase
RA NZ_CP022686 3,424,795 A→C 100% intergenic (‑41/+27) yjiJ ← / ← yjiK MFS transporter/YjiK family protein
RA NZ_CP022686 3,451,845 A→C 11.2% E278D (GAA→GAC lgoD → L‑galactonate oxidoreductase
RA NZ_CP022686 3,461,127 A→C 14.1% noncoding (65/87 nt) CIW80_RS17335 ← tRNA‑Leu
RA NZ_CP022686 3,461,231 C→G 14.9% noncoding (82/87 nt) CIW80_RS17340 ← tRNA‑Leu
RA NZ_CP022686 3,493,370 T→G 7.4% K195N (AAA→AAC arcA ← two‑component system response regulator ArcA
RA NZ_CP022686 3,504,054 T→G 9.4% V204G (GTT→GGT)  tal → transaldolase
RA NZ_CP022686 3,516,073 C→G 9.9% G212G (GGC→GGG nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,516,093 T→G 11.0% V219G (GTG→GGG)  nhaA → Na+/H+ antiporter NhaA
RA NZ_CP022686 3,516,129 T→G 7.6% V231G (GTC→GGC)  nhaA → Na+/H+ antiporter NhaA
MC JC NZ_CP022686 3,559,837 Δ2,343 bp 100% coding (10‑2352/2352 nt) polB ← DNA polymerase II
RA NZ_CP022686 3,583,665 A→C 6.2% Q342H (CAA→CAC ilvI → acetolactate synthase 3 large subunit
RA NZ_CP022686 3,604,564 G→T 8.7% intergenic (+80/‑151) lpxC → / → secM UDP‑3‑O‑acyl‑N‑acetylglucosamine deacetylase/secA translation cis‑regulator SecM
RA NZ_CP022686 3,625,701 T→G 7.0% G156G (GGT→GGG aceE → pyruvate dehydrogenase (acetyl‑transferring), homodimeric type
RA NZ_CP022686 3,656,188 A→G 100% S549P (TCC→CCC)  htrE ← outer membrane usher protein
RA NZ_CP022686 3,714,300 T→G 8.5% W261G (TGG→GGG)  tilS → tRNA lysidine(34) synthetase TilS
RA NZ_CP022686 3,734,367 T→G 17.9% F225V (TTT→GTT)  yafD → endonuclease/exonuclease/phosphatase family protein
RA NZ_CP022686 3,770,524 T→G 6.7% intergenic (‑260/+203) CIW80_RS26265 ← / ← CIW80_RS18890 ATP‑binding protein/hypothetical protein
RA NZ_CP022686 3,792,805 A→G 100% Y421C (TAC→TGC)  fucP → L‑fucose:H+ symporter permease
RA NZ_CP022686 3,797,495 T→G 9.2% G134G (GGT→GGG CIW80_RS19065 → LacI family DNA‑binding transcriptional regulator
RA NZ_CP022686 3,800,981 A→C 8.1% pseudogene (1257/1773 nt) CIW80_RS19080 → ShlB/FhaC/HecB family hemolysin secretion/activation protein
MC JC NZ_CP022686 3,844,426 Δ1,036 bp 100% coding (2‑1037/1056 nt) dinB → DNA polymerase IV
RA NZ_CP022686 3,932,443 C→A 14.9% E184* (GAA→TAA)  lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,932,468 A→C 7.7% A175A (GCT→GCG lacA ← galactoside O‑acetyltransferase
RA NZ_CP022686 3,940,724 A→C 8.4% V308G (GTA→GGA)  frmA ← S‑(hydroxymethyl)glutathione dehydrogenase
RA NZ_CP022686 3,977,656 A→C 7.1% K88N (AAA→AAC phoR → phosphate regulon sensor histidine kinase PhoR
RA NZ_CP022686 3,981,933 T→G 13.0% intergenic (+70/‑86) proY → / → malZ proline‑specific permease ProY/maltodextrin glucosidase
RA NZ_CP022686 4,014,238 C→G 11.3% intergenic (+84/‑260) bolA → / → tig transcriptional regulator BolA/trigger factor
RA NZ_CP022686 4,056,962 C→A 23.6% F137L (TTC→TTA adk → adenylate kinase
RA NZ_CP022686 4,056,978 G→T 16.0% E143* (GAA→TAA)  adk → adenylate kinase
RA NZ_CP022686 4,056,983 C→A 9.3% G144G (GGC→GGA adk → adenylate kinase
RA NZ_CP022686 4,074,899 A→C 6.8% V24G (GTG→GGG)  CIW80_RS20380 ← hypothetical protein
RA NZ_CP022686 4,133,850 T→G 13.1% K113N (AAA→AAC ybdF ← MmcQ/YjbR family DNA‑binding protein
RA NZ_CP022686 4,213,260 T→G 6.2% C188W (TGT→TGG ubiF → 3‑demethoxyubiquinol 3‑hydroxylase
RA NZ_CP022686 4,412,302 T→G 9.9% I235L (ATC→CTC)  ybjT ← SDR family oxidoreductase
RA NZ_CP022686 4,440,297 T→A 10.4% V785D (GTT→GAT)  ftsK → DNA translocase FtsK
RA NZ_CP022686 4,440,334 A→T 23.1% Q797H (CAA→CAT ftsK → DNA translocase FtsK
RA NZ_CP022686 4,571,417 T→G 9.9% A66A (GCA→GCC pgaC ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine synthase
RA NZ_CP022686 4,632,182 T→G 10.0% R276R (CGT→CGG mchF → microcin export transporter peptidase/ATP‑binding subunit MchF
RA NZ_CP022686 4,638,444 A→C 5.6% intergenic (+17/‑355) CIW80_RS23235 → / → focA S/F1C fimbrial major subunit operon transcriptional regulator/F1C fimbrial major subunit FocA
RA NZ_CP022686 4,678,047 A→C 24.3% intergenic (+45/‑76) agn43 → / → CIW80_RS23450 autotransporter adhesin Ag43/hypothetical protein
RA NZ_CP022686 4,764,877 T→G 11.9% intergenic (‑57/‑204) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,764,904 G→T 12.2% intergenic (‑84/‑177) ycfT ← / → lolC acyltransferase family protein/lipoprotein‑releasing ABC transporter permease subunit LolC
RA NZ_CP022686 4,804,041 C→A 7.1% A614E (GCA→GAA)  CIW80_RS24225 → phage terminase large subunit family protein
RA NZ_CP022686 4,809,441 T→G 10.7% G50G (GGT→GGG CIW80_RS24260 → head‑tail joining protein
RA NZ_CP022686 4,814,990 T→G 12.9% F71V (TTT→GTT)  CIW80_RS24295 → phage tail protein
RA NZ_CP022686 4,815,879 T→G 10.4% F23V (TTC→GTC)  CIW80_RS24305 → C40 family peptidase
MC JC NZ_CP022686 4,844,868 Δ1,662 bp 100% [umuD][umuC] [umuD], [umuC]
RA NZ_CP022686 4,870,912 Δ1 bp 100% intergenic (‑108/‑120) dhaK ← / → dhaR dihydroxyacetone kinase subunit DhaK/dihydroxyacetone kinase operon transcriptional regulator DhaR
RA NZ_CP022686 4,888,297 T→G 7.4% F78V (TTT→GTT)  chaC → glutathione‑specific gamma‑glutamylcyclotransferase
RA NZ_CP022686 4,910,213 A→C 8.7% F48V (TTT→GTT)  purU ← formyltetrahydrofolate deformylase
RA NZ_CP022686 4,951,512 G→T 15.9% D36Y (GAT→TAT)  topA → type I DNA topoisomerase
RA NZ_CP022686 5,000,034 T→G 10.6% L162R (CTC→CGC)  CIW80_RS25310 → ABC transporter ATP‑binding protein
RA NZ_CP022686 5,041,191 G→A 6.7% A274A (GCG→GCA CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,289 T→C 10.5% N640N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,042,304 T→C 11.1% N645N (AAT→AAC CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,043,347 A→G 6.0% N993S (AAT→AGT)  CIW80_RS25525 → EntS/YbdA MFS transporter
RA NZ_CP022686 5,044,014 T→A 13.9% V1215V (GTT→GTA CIW80_RS25525 → EntS/YbdA MFS transporter
RA pHH‑29_RBS 1,534 G→A 100% W12* (TGG→TGA Aldafermin (NGM282) →

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_MW240712 189–2618 3085–2956 339–2897 14387 [13396] [14111] 14327 JNG15_RS00005–mobA JNG15_RS00005,JNG15_RS00010,JNG15_RS00015,mobA
* * ÷ pHH‑29_RBS 2775–2841 5511–3105 265–2737 6651 [6576] [6534] 6637 putative HTH domain protein (MarR‑like)–hypothetical protein putative HTH domain protein (MarR‑like),putative HTH domain protein,uncharacterized protein,hypothetical protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP022686 262466 =1 (0.010)172 (0.880) 71/248 NT 99.4% coding (320/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1524130 NA (NA)intergenic (+8/+232) CIW80_RS07590/syd IS3‑like element ISEc52 family transposase/SecY‑interacting protein
* ? NZ_CP022686 = 2624681 (0.010)173 (0.880) 80/248 NT 99.4% coding (322/1647 nt) fadK medium‑chain fatty‑acid‑‑CoA ligase
?NZ_CP022686 = 1761697 NA (NA)intergenic (‑72/‑70) CIW80_RS08710/CIW80_RS08720 IS3‑like element ISEc52 family transposase/IS3‑like element IS600 family transposase