breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 502 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_007779357,1040TC53.8% 1.3 / 36.0 26K271E (AAA→GAA) cynRtranscriptional regulator CynR
*NC_007779357,1140CA51.7% 1.7 / 33.3 29L267F (TTG→TTTcynRtranscriptional regulator CynR
*NC_0077791,273,4040AT48.7% 119.4 / 130.6 158V8E (GTA→GAA) chaAsodium‑potassium/proton antiporter ChaA
*NC_0077791,273,3990TG46.0% 196.6 / 70.3 151T10P (ACC→CCC) chaAsodium‑potassium/proton antiporter ChaA
*NC_0077794,434,6640TG44.9% 178.0 / 54.0 120L41V (TTG→GTG) cycAD‑serine/D‑alanine/glycine transporter
*NC_0077791,097,5800CT41.4% 31.9 / 114.8 87intergenic (+329/+45)Y75_RS05375/Y75_RS05385protein YcdU/RtT sRNA
*NC_0077791,273,4100TC40.8% 240.5 / 53.2 165E6G (GAG→GGG) chaAsodium‑potassium/proton antiporter ChaA
*NC_0077792,391,5320GA40.6% 128.0 / 108.0 123intergenic (+31/‑72)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NC_0077793,974,7130GT40.4% 251.5 / 81.2 152A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NC_007779344,5520CA40.4% 207.7 / 71.8 114intergenic (+337/‑76)Y75_RS01685/Y75_RS01690protein YahL/protein YahM
*NC_007779121,5070AT39.7% 179.5 / 64.0 116L15L (CTT→CTAaroParomatic amino acid transporter AroP
*NC_0077793,880,0310TG39.0% 108.0 / 40.7 95V289G (GTG→GGG) selAL‑seryl‑tRNA(Sec) selenium transferase
*NC_007779753,5470TA38.6% 214.7 / 70.1 141intergenic (‑330/+60)ybgD/gltAfimbrial protein/citrate synthase
*NC_007779357,0990AT38.5% 11.5 / 10.4 26N272K (AAT→AAAcynRtranscriptional regulator CynR
*NC_0077793,205,0910CT37.5% 144.7 / 100.4 147intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator TtdR/L(+)‑tartrate dehydratase subunit alpha
*NC_0077793,777,7540AC37.0% 78.8 / 29.2 93pseudogene (943/1617 nt)Y75_RS18735PTS sugar transporter subunit IIB
*NC_0077791,273,4220GT36.2% 329.3 / 61.7 160S2* (TCA→TAA) chaAsodium‑potassium/proton antiporter ChaA
*NC_0077791,555,7140TG36.1% 216.3 / 30.4 146Y557S (TAC→TCC) maeAmalate dehydrogenase
*NC_0077793,535,1740AC35.3% 286.2 / 23.8 138intergenic (‑183/+77)kdgT/sodA2‑keto‑3‑deoxygluconate transporter/superoxide dismutase [Mn]
*NC_0077793,796,1960AC35.0% 242.0 / 30.3 120F86V (TTT→GTT) adeDadenine deaminase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 4547362 =194 (1.020)8 (0.040) 8/448 10.4 4.3% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NC_007779 4547676 = 169 (0.920)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NC_007779 = 4547370205 (1.080)5 (0.030) 3/448 13.5 2.7% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NC_007779 = 4547666 169 (0.920)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NC_007779 = 2564595171 (0.900)3 (0.020) 3/450 13.5 1.8% coding (946/1404 nt) eutA ethanolamine ammonia‑lyase reactivating factor EutA
?NC_007779 = 2564610 163 (0.890)coding (931/1404 nt) eutA ethanolamine ammonia‑lyase reactivating factor EutA
* ? NC_007779 = 1374998240 (1.260)3 (0.020) 3/450 13.5 1.3% coding (63/882 nt) ycjO sugar ABC transporter permease
?NC_007779 = 1375006 232 (1.260)coding (71/882 nt) ycjO sugar ABC transporter permease
* ? NC_007779 1544936 =235 (1.240)3 (0.020) 3/466 13.8 1.7% coding (839/1389 nt) narU nitrate/nitrite transporter NarU
?NC_007779 = 3232082 110 (0.580)coding (1762/2019 nt) fadH NADPH‑dependent 2,4‑dienoyl‑CoA reductase