breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Read File Information

read filereadsbasespassed filtersaveragelongestmapped
errorsSRR19973057_R1.good.fq5,205,379775,434,576100.0%149.0 bases149 bases96.6%
errorsSRR19973057_R2.good.fq5,205,379775,434,576100.0%149.0 bases149 bases96.5%
total10,410,7581,550,869,152100.0%149.0 bases149 bases96.6%

Reference Sequence Information

seq idlengthfit meanfit dispersion% mapped readsdescription
coveragedistributionCP0092734,631,469320.13.2100.0%Escherichia coli BW25113, complete genome.
total4,631,469100.0%

fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.

New Junction Evidence

Junction Candidates Tested

optionlimitactual
Number of alignment pairs examined for constructing junction candidates≤ 10000025403
Coverage evenness (position-hash) score of junction candidates≥ 2≥ 2
Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold100 ≤ n ≤ 5000107
Total length of all junction candidates (factor times the reference genome length)≤ 0.10.007

Junction Skew Score Calculation

reference sequencepr(no read start)
CP0092730.48025

pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.

Final Junction Predictions

optionvalue
Coverage evenness (position-hash) score of predicted junctions must be≥ 3
Minimum probablilty assigned that no mapped read will start at a given position and strand for junction prediction0.1
Junction allow suboptimal matchesFALSE
Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be≤ 0
Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction≥ 6

Read Alignment Evidence

optionvalue
ModeFull Polymorphism
Ploidy1 (haploid)
Consensus mutation E-value cutoff10
Consensus frequency cutoff0.75
Consensus minimum variant coverage each strandOFF
Consensus minimum total coverage each strandOFF
Consensus minimum variant coverageOFF
Consensus minimum total coverageOFF
Polymorphism E-value cutoff2
Polymorphism frequency cutoff0.05
Polymorphism minimum variant coverage each strand2
Polymorphism minimum total coverage each strandOFF
Polymorphism minimum variant coverageOFF
Polymorphism minimum total coverageOFF
Polymorphism bias cutoffOFF
Predict indel polymorphismsYES
Skip indel polymorphisms in homopolymers runs of ≥3 bases
Skip base substitutions when they create a homopolymer flanked on each side by ≥5 bases

Software Versions

programversion
bowtie22.2.6
R3.4.4

Execution Times

stepstartendelapsed
Read and reference sequence file input09:45:13 24 Jan 202309:47:50 24 Jan 20232 minutes 37 seconds
Read alignment to reference genome09:47:50 24 Jan 202310:07:12 24 Jan 202319 minutes 22 seconds
Preprocessing alignments for candidate junction identification10:07:12 24 Jan 202310:09:27 24 Jan 20232 minutes 15 seconds
Preliminary analysis of coverage distribution10:09:27 24 Jan 202310:13:39 24 Jan 20234 minutes 12 seconds
Identifying junction candidates10:13:39 24 Jan 202310:13:42 24 Jan 20233 seconds
Re-alignment to junction candidates10:13:42 24 Jan 202310:18:55 24 Jan 20235 minutes 13 seconds
Resolving best read alignments10:18:55 24 Jan 202310:22:28 24 Jan 20233 minutes 33 seconds
Creating BAM files10:22:28 24 Jan 202310:26:15 24 Jan 20233 minutes 47 seconds
Tabulating error counts10:26:15 24 Jan 202310:28:29 24 Jan 20232 minutes 14 seconds
Re-calibrating base error rates10:28:29 24 Jan 202310:28:30 24 Jan 20231 second
Examining read alignment evidence10:28:30 24 Jan 202312:11:46 24 Jan 20231 hour 43 minutes 16 seconds
Polymorphism statistics12:11:46 24 Jan 202312:11:50 24 Jan 20234 seconds
Output12:11:50 24 Jan 202312:13:11 24 Jan 20231 minute 21 seconds
Total 2 hours 27 minutes 58 seconds