breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 57,069 G→T A14D (GCC→GAC)  lptD ← LPS assembly protein LptD
RA 874,860 G→A M221I (ATG→ATA yliI → aldose sugar dehydrogenase YliI
RA 1,431,900 G→A Q1048* (CAG→TAG)  nifJ ← pyruvate:ferredoxin (flavodoxin) oxidoreductase
RA 1,913,385 T→G V540G (GTT→GGT)  yebT → lipid‑binding membrane homeostasis protein YebT
JC 3,300,211 (CGCACGACC)1→2 coding (1009/1890 nt) deaD ← ATP‑dependent RNA helicase DeaD
RA 3,961,365 (A)7→6 coding (90/1104 nt) wecA → UDP‑N‑acetylglucosamine‑‑undecaprenyl‑phosphate N‑acetylglucosaminephosphotransferase
RA 4,323,527 G→T R81S (CGC→AGC)  basR ← two‑component system response regulator BasR

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 3624595 3646446–3645390 20796–21852 65 [0] [24] 25 [yhiJ]–[BW25113_RS18160] [yhiJ],BW25113_RS18065,yhiM,yhiN,pitA,uspB,uspA,BW25113_RS26225,BW25113_RS26230,dtpB,rsmJ,prlC,rlmJ,gorA,dinQ,arsR,arsB,arsC,BW25113_RS18155,[BW25113_RS18160]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 360104 =29 (0.520)33 (0.600) 31/494 1.6 50.0% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 37 (0.670)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =8 (0.140)41 (0.790) 40/462 0.6 84.1% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 8 (0.150)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032618 (0.140)43 (0.830) 43/462 0.4 84.8% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 8 (0.150)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 36245940 (0.000)65 (1.180) 55/494 0.1 100% coding (1357/1623 nt) yhiJ YhiJ/YhiL family protein
?NZ_CP009273 3645396 = NA (NA)pseudogene (746/747 nt) BW25113_RS18155 hypothetical protein