| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | REL606 | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 1733288 = | 44 (0.260) | 167 (1.060) | 62/68 | 0.1 | 83.4% | coding (324/1413 nt) | pykF | pyruvate kinase |
| ? | REL606 | = 1733295 | 25 (0.160) | coding (331/1413 nt) | pykF | pyruvate kinase | |||||
Only 100 of 348 total aligned reads displayed.
GTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCG > REL606/1733259‑1733331 | gTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATg < 2:893899/37‑1 (MQ=255)gTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATg > 2:7795733/1‑37 (MQ=255)gTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATg > 2:10981319/1‑37 (MQ=255)caacagcgaaatggttgcggtaacgtatgaacgtatg < 1:8226252‑M1/8‑1 (MQ=255)caacagcgaaatggttgcggtaacgtatgaacgtatg > 2:4603369‑M1/30‑37 (MQ=255)caacagagaaatggttgcggtaacgtatgaacgtatg < 2:1934179‑M1/8‑1 (MQ=255) aacagcgaaatggttgcggtaacgtatgaacgtatga < 1:8926781‑M1/9‑1 (MQ=255) aacagcgaaatggttgcggtaacgtatgaacgtatga < 1:9039265‑M1/9‑1 (MQ=255) aacagcgaaatggttgcggtaacgtatgaacgtatga > 2:2994915‑M1/29‑37 (MQ=255) tATCGGCAACAGCGAAATGGTTGCGGTAACGTATTaa > 1:2937640/1‑37 (MQ=255) cagcgaaatggttgcggtaacgtatgAACGTATGAAg < 2:9879965‑M1/11‑1 (MQ=255) cagcgaaatggttgcggtaacgtatgAACGTATGAAg > 2:1536981‑M1/27‑37 (MQ=255) aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGaac > 2:3513104‑M2/1‑34 (MQ=255) aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGaac > 2:3982839‑M2/1‑34 (MQ=255) aTCGGCAACAGCGAAATGGTTGCGGTAACGTATGaac < 2:6889966‑M2/37‑4 (MQ=255) tCGGCAACAGCGAAATGGTTGCGGTAACGTATGaacg < 2:7592355‑M2/37‑5 (MQ=255) tCGGCAACAGCGAAATGGTTGCGGGAACGCACGaacg < 1:1031916‑M2/37‑5 (MQ=255) tCGGCAAAAGCGAAATGGTTGCGGTAACGTATGaacg < 1:9005068‑M2/37‑5 (MQ=255) agcgaaatggttgcggtaacgtatgAACGTATGAAgg < 1:6221250‑M1/12‑1 (MQ=255) gcgaaatggttgcggtaacgtatgAACGTATGAAGGt < 1:9546815‑M1/13‑1 (MQ=255) ggCAACAGCGAAATGGTTGCGGTAACGTATGaacgta > 2:1165708‑M2/1‑31 (MQ=255) ggCAACAGCGAAATGGTTGCGGTAACGTATGaacgta > 1:10178986‑M2/1‑31 (MQ=255) ggCAACAGCGAAATGGTTGAGGTAACGTATGaacgta < 1:249950‑M2/37‑7 (MQ=255) cgaaatggttgcggtaacgtatgAACGTATGAAGGtt < 2:3596166‑M1/14‑1 (MQ=255) gaaatggttgcggtaacgtatgAACGTATGAAGGttt > 2:4707994‑M1/23‑37 (MQ=255) gaaatggttgcggtaacgtatgAACGTATGAAGGttt > 2:8126965‑M1/23‑37 (MQ=255) gCAACAGCGAAATGGTTGCGGTAACGTATGaacgtat > 1:6160069‑M2/1‑30 (MQ=255) cAACAGCGAAATGGTTGCGGTAACGTATGaacgtatg < 1:7661643‑M2/37‑9 (MQ=255) cAACAGCGAAATGGTTGCGGTAACGTATGaacgtatg < 1:8862834‑M2/37‑9 (MQ=255) aatggttgcggtaacgtatgAACGTATGAAGGTTTCa < 2:5520566‑M1/17‑1 (MQ=255) aaCAGCGAAATGGTTGCGGTAACGTATGaacgtatga > 2:413987‑M2/1‑28 (MQ=255) atggttgcggtaacgtatgAACGTATGAAGGTTTCac < 2:6948048‑M1/18‑1 (MQ=255) aCAGCGAAATGGTTGCGGTAACGTATGaacgtatgaa < 1:6601678‑M2/37‑11 (MQ=255) cAGCGAAATGGTTGCGGTAACGTATGaacgtatgaag < 1:6836907‑M2/37‑12 (MQ=255) cAGCGAAATGGTTGCGGTAACGTATGaacgtatgaag > 1:6875851‑M2/1‑26 (MQ=255) cAGCGAAATGGTTGCGGTAACGTATGaacgtatgaag < 1:1161049‑M2/37‑12 (MQ=255) cAGCGAAATGGTTGCGGTAACGTATGaacgtatgaag > 2:659777‑M2/1‑26 (MQ=255) ggttgcggtaacgtatgAACGTATGAAGGTTTCacta < 1:6345213‑M1/20‑1 (MQ=255) aGCGAAATGGTTGCGGTAACGTATGaacgtatgaagg > 2:2527260‑M2/1‑25 (MQ=255) gttgcggtaacgtatgAACGTATGAAGGTTTCactac < 2:4404044‑M1/21‑1 (MQ=255) cGAAATGGGTGCGGTAACGTATGaaagtatggaggtt < 1:9982849‑M2/37‑15 (MQ=255) tgcggtaacgtatgAACGTATGAAGGTTTCACTACTg < 1:4459071‑M1/23‑1 (MQ=255) gAAATGGTTGCGGTAACGTATGaacgtatgaaggttt < 2:3267398‑M2/37‑16 (MQ=255) gcggtaacgtatgAACGTATGAAGGTTTCACTACTGa > 2:2191455‑M1/14‑37 (MQ=255) cggtaacgtatgAACGTATGAAGGTTTCACTACTGAc > 2:3815429‑M1/13‑37 (MQ=255) cggtaacgtatgAACGTATGAAGGTTTCACTACTGAc > 1:5472403‑M1/13‑37 (MQ=255) aaTGGTTGCGGTAACGTATGaacgtatgaaggtttca > 2:5544082‑M2/1‑20 (MQ=255) aaTGGTTGCGGTAACGTATGaacgtatgaaggtttca > 1:410851‑M2/1‑20 (MQ=255) ggtaacgtatgAACGTATGAAGGTTTCACTACTGAcc > 2:10970601‑M1/12‑37 (MQ=255) ggtaacgtatgAACGTATGAAGGTTTCACTACTGAcc < 1:8802019‑M1/26‑1 (MQ=255) ggtaacgtatgAACGTATGAAGGTTTCACTACTGAcc > 1:114275‑M1/12‑37 (MQ=255) tGGTTGCGGTAACGTATGaacgtatgaaggtttcact < 2:8532045‑M2/37‑20 (MQ=255) gtaacgtatgAACGTATGAAGGTTTCACTACTGACct > 1:2228402‑M1/11‑37 (MQ=255) taacgtatgAACGTATGAAGGTTTCACTACTGACctg < 1:7027845‑M1/28‑1 (MQ=255) taacgtatgAACGTATGAAGGTTTCACGACTGACctg < 1:558380‑M1/28‑1 (MQ=255) gTTGCGGTAACGTATGaacgtatgaaggtttcactac > 1:4272196‑M2/1‑16 (MQ=255) gTTGCGGTAACGTATGaacgtatgaaggtttcactac < 2:9587296‑M2/37‑22 (MQ=255) aacgtatgAACGTATGAAGGTTTCACTACTGACctgt < 1:994375‑M1/29‑1 (MQ=255) aacgtatgAACGTATGAAGGTTTCACTACTGACctgt < 1:799820‑M1/29‑1 (MQ=255) aacgtatgAACGTATGAAGGTTTCACTACTGACctgt < 1:4804349‑M1/29‑1 (MQ=255) ttGCGGTAACGTATGaacgtatgaaggtttcactact < 1:4382645‑M2/37‑23 (MQ=255) acgtatgAACGTATGAAGGTTTCACTACTGACctgtc < 1:7461672‑M1/30‑1 (MQ=255) tGCGGTAACGTATGaacgtatgaaggtttcactactg > 2:10481213‑M2/1‑14 (MQ=255) gtatgAACGTATGAAGGTTTCACTACTGACctgtctg > 2:2060781‑M1/6‑37 (MQ=255) gtatgAACGTATGAAGGTTTCACTACTGACctgtctg < 1:3824335‑M1/32‑1 (MQ=255) gCGGTAACGTATGaacgtatgaaggtttcactactga < 1:2424569‑M2/37‑25 (MQ=255) gCGGTAACGTATGaacgtatgaaggtttcactactga > 1:4572057‑M2/1‑13 (MQ=255) tatgAACGTATGAAGGTTTCACTACTGACctgtctgt > 2:520126‑M1/5‑37 (MQ=255) tatgAACGTATGAAGGTTTCACTACTGACctgtctgt < 1:2988229‑M1/33‑1 (MQ=255) tatgAACGTATGAAGGTTTCACTACTGACctgtctgt < 2:7104240‑M1/33‑1 (MQ=255) tatgAACGTATGAAGGTTTCACTACTGACctgtctgt > 2:4860502‑M1/5‑37 (MQ=255) cGGTAACGTATGaacgtattaaggtttcactactgac > 1:3588490‑M2/1‑12 (MQ=255) cGGTAACGTATGaacgtatgaaggtttcactactgac < 1:7910199‑M2/37‑26 (MQ=255) ggTAACGTATGaacgtatgaaggtttcactactgacc < 1:4838142‑M2/37‑27 (MQ=255) ggTAACGTATGaacgtatgaaggtttcactactgacc > 2:3394677‑M2/1‑11 (MQ=255) atgAACGTATGAAGGTTTCACTACTGACCTGTCTGtt < 1:4656034‑M1/34‑1 (MQ=255) atgAACGTATGAAGGTTTCACTACTGACCTGTCTGtt < 1:10139953‑M1/34‑1 (MQ=255) tgAACGTATGAAGGTTTCACTACTGACCTGTCTGTTg > 1:7840657‑M1/3‑37 (MQ=255) gTAGCGTATGaacgtatgaaggtttcactactgacct > 2:6758301‑M2/1‑10 (MQ=255) gTAACGTATGaacgtatgaaggtttcactactgacct > 1:9076634‑M2/1‑10 (MQ=255) gTAACGTATGaacgtatgaaggtttcactactgacct > 1:8444204‑M2/1‑10 (MQ=255) gTAACGTATGaacgtatgaaggtttcactactgacct > 2:1573589‑M2/1‑10 (MQ=255) tAACGTATGaacgtatgaaggtttcactactgacctg > 2:10057260‑M2/1‑9 (MQ=255) acgtatgaacgtatgaaggtttcactactgacctgtc > 2:4571714‑M2/1‑7 (MQ=255) cgtatgaacgtatgaaggtttcactactgacctgtct > 1:9259183‑M2/1‑6 (MQ=255) cGTATGAAGGTTTCACTACTGACCTGTCTGTTTGCaa < 2:5649499/37‑1 (MQ=255) cGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCaa < 2:7191409/37‑1 (MQ=255) tatgaacgtatgaaggtttcactactgacctgtctgt < 2:10177934‑M2/37‑34 (MQ=255) atgaacgtatgaaggtttcactactgacctgtctgtt < 1:5173324‑M2/37‑35 (MQ=255) atgaacgtatgaaggtttcactactgacctgtctgtt < 2:4966823‑M2/37‑35 (MQ=255) atgaacgtatgaaggtttcactactgacctgtctgtt < 2:2231288‑M2/37‑35 (MQ=255) atgaacgtatgaaggtttcactactgacctgtctgtt < 1:7302042‑M2/37‑35 (MQ=255) aTGAAGGTTTCACTACTGACCTGTCTGTTGGCAacac < 1:3054294/37‑1 (MQ=255) tgaacttatgaaggtttccctactgacctgtctgttg > 2:5711624‑M2/1‑2 (MQ=255) tgaacgtatgaaggtttcactactgacctgtctgttg > 2:8354281‑M2/1‑2 (MQ=255) tGAAGTTTTCACTACTGACCTGTCTGTTGGCAACAcc < 2:5136902/37‑1 (MQ=255) tGAAGGTTTCACTACTGACCTGTCTGTTGGCAACAcc > 1:9832079/1‑37 (MQ=255) gaacgtatgaaggtttcactactgacctgtctgttgg > 1:314251‑M2/1‑1 (MQ=255) gaacgtatgaaggtttcactactgacctgtctgttgg > 1:7091124‑M2/1‑1 (MQ=255) gAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCg < 1:1747676/37‑1 (MQ=255) | GTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCG > REL606/1733259‑1733331 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |