BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	433,584	Δ16 bp	coding (79‑94/273 nt)	hupB →	HU, DNA‑binding transcriptional regulator, beta subunit

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	433584	433599	16	362 [0]	[2] 364	hupB	HU, DNA‑binding transcriptional regulator, beta subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 433583	0 (0.000)	358 (1.130)	126/150	0.1	99.7%	coding (78/273 nt)	hupB	HU, DNA‑binding transcriptional regulator, beta subunit
*	?	REL606	433600 =	2 (0.010)	358 (1.130)	126/150	0.1	99.7%	coding (95/273 nt)	hupB	HU, DNA‑binding transcriptional regulator, beta subunit

Only 100 of 362 total aligned reads displayed.

GAATAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGC  >  REL606/433508‑433657

gAATAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGAt                                                                            >  1:1531343/1‑76 (MQ=255)

 aaTAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATt                                                                           >  2:9460461‑M1/1‑75 (MQ=255)

 aaTAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATt                                                                           <  2:3983094‑M1/76‑2 (MQ=255)

   tAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaa                                                                         <  1:4075633‑M1/76‑4 (MQ=255)

    aaaTCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaac                                                                        >  1:7369698‑M1/1‑72 (MQ=255)

       tctcAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactga                                                                     <  1:5700826‑M1/76‑8 (MQ=255)

        ctcAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaa                                                                    >  1:1095743‑M1/1‑68 (MQ=255)

        ctcAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaa                                                                    <  2:1712103‑M1/76‑9 (MQ=255)

         tcAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaaatgaat                                                                   >  1:8093159‑M1/1‑67 (MQ=255)

          cAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatc                                                                  >  1:10005272‑M1/1‑66 (MQ=255)

          cAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatc                                                                  <  1:5671395‑M1/76‑11 (MQ=255)

          cAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatc                                                                  <  2:4798879‑M1/76‑11 (MQ=255)

            aTTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctc                                                                <  1:9427387‑M1/76‑13 (MQ=255)

            aTTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctc                                                                <  1:4916026‑M1/76‑13 (MQ=255)

            aTTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctc                                                                >  1:4334337‑M1/1‑64 (MQ=255)

              tGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctg                                                              >  2:4550456‑M1/1‑62 (MQ=255)

               gATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGGTAGATtaactgaatctctga                                                             >  2:9016653‑M1/1‑61 (MQ=255)

                aTCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaa                                                            <  1:3740449‑M1/76‑17 (MQ=255)

                aTCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaa                                                            <  1:9916356‑M1/76‑17 (MQ=255)

                aTCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaa                                                            <  1:2210281‑M1/76‑17 (MQ=255)

                aTCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaa                                                            <  2:3843813‑M1/76‑17 (MQ=255)

                 tCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaa                                                           <  1:77463‑M1/76‑18 (MQ=255)

                  cGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaag                                                          <  2:7134308‑M1/76‑19 (MQ=255)

                  cGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGGTAGATtaactgaatctctgaaag                                                          >  2:2872556‑M1/1‑58 (MQ=255)

                  cGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGGGCGTTAGATtaactgaatctctgaaag                                                          >  2:5329237‑M1/1‑58 (MQ=255)

                  cGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGGGCGTTAGATtaaatgaatctttgtaag                                                          >  1:2769038‑M1/1‑58 (MQ=255)

                   gACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaaga                                                         >  1:3598012‑M1/1‑57 (MQ=255)

                    aCAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaa                                                        <  2:1905647‑M1/76‑21 (MQ=255)

                    aCAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGGTAGATtaactgaatctctgaaagaa                                                        >  1:4507330‑M1/1‑56 (MQ=255)

                      aacattgCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaagg                                                      >  1:466353‑M1/4‑54 (MQ=255)

                       aGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATttactgaatctctgaaagaaggg                                                     >  2:9630877‑M1/1‑53 (MQ=255)

                        gATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaagggg                                                    <  1:6508972‑M1/76‑25 (MQ=255)

                        gATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaagggg                                                    <  1:3320912‑M1/76‑25 (MQ=255)

                          ttGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggat                                                  <  2:4885383‑M1/76‑27 (MQ=255)

                           tgctgcAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatg                                                 >  1:7533683‑M1/1‑49 (MQ=255)

                           tgctgcAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGGTAGATtaactgaatctctgaaagaaggggatg                                                 >  2:3210866‑M1/1‑49 (MQ=255)

                            gctgcAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatga                                                >  1:4881428‑M1/1‑48 (MQ=255)

                              tgcAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatg                                              <  2:6546482‑M1/76‑31 (MQ=255)

                               gcAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGGTAGGTtaactgaatctctgaaagaaggggatgatga                                             >  2:1025365‑M1/1‑45 (MQ=255)

                                cAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgta                                            >  2:9349643‑M1/1‑44 (MQ=255)

                                 aGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtag                                           <  2:1316849‑M1/76‑34 (MQ=255)

                                 aGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtag                                           <  2:9841942‑M1/76‑34 (MQ=255)

                                  ggggCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagc                                          <  1:9101491‑M1/76‑35 (MQ=255)

                                  ggggCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagc                                          <  2:1228153‑M1/76‑35 (MQ=255)

                                  ggggCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagc                                          <  1:5077300‑M1/76‑35 (MQ=255)

                                   gggCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagca                                         <  2:1063745‑M1/76‑36 (MQ=255)

                                    ggCTGATATCTCTAAAGCTGCGGCTGGCCGGGCGTTAGATtaactgaatctcttaaagaaggggatgatgaagccc                                        >  1:1722090‑M1/1‑40 (MQ=255)

                                     gCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcact                                       >  2:8728511‑M1/1‑39 (MQ=255)

                                     gCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcact                                       >  2:5202839‑M1/1‑39 (MQ=255)

                                      cTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactg                                      >  2:7486553‑M1/1‑38 (MQ=255)

                                      cTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactg                                      >  2:618587‑M1/1‑38 (MQ=255)

                                      cTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactg                                      <  1:4271378‑M1/76‑39 (MQ=255)

                                      cTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactg                                      >  2:2756174‑M1/1‑38 (MQ=255)

                                       tGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactgg                                     >  2:7751152‑M1/1‑37 (MQ=255)

                                       tGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactgg                                     >  2:4969848‑M1/1‑37 (MQ=255)

                                       tGATATCTCTAAAGCTGCGGCTGGCCGCGCGTTAGATtaactgtatctctgaaagaaggggatgttgttgcgcttg                                     >  2:8407670‑M1/1‑37 (MQ=255)

                                        gATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggt                                    >  1:10180309‑M1/1‑36 (MQ=255)

                                        gATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggt                                    >  2:7664150‑M1/1‑36 (MQ=255)

                                        gATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggt                                    >  1:9507905‑M1/1‑36 (MQ=255)

                                          tatCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtag                                  <  1:6093570‑M1/76‑43 (MQ=255)

                                              tctAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttt                              <  2:4614879‑M1/76‑47 (MQ=255)

                                               ctAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggtttt                             <  1:8796611‑M1/76‑48 (MQ=255)

                                               ctAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggtttt                             <  1:5781868‑M1/76‑48 (MQ=255)

                                               ctAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtagggttt                             >  1:6627935‑M1/1‑29 (MQ=255)

                                                tAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttg                            <  2:7883515‑M1/76‑49 (MQ=255)

                                                tAAAGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtagggtttg                            >  1:6305229‑M1/1‑28 (MQ=255)

                                                 aaaGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttgg                           >  2:2307217‑M1/1‑27 (MQ=255)

                                                 aaaGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtagggtttgg                           >  1:4164591‑M1/1‑27 (MQ=255)

                                                 aaaGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactgggaggggttgg                           >  2:5616672‑M1/1‑27 (MQ=255)

                                                  aaGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggt                          <  1:3523096‑M1/76‑51 (MQ=255)

                                                  aaGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggt                          <  2:9131784‑M1/76‑51 (MQ=255)

                                                   aGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggta                         <  1:5442114‑M1/76‑52 (MQ=255)

                                                   aGCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggta                         <  1:2403388‑M1/76‑52 (MQ=255)

                                                    gCTGCGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtagggtttggtac                        >  2:1446299‑M1/1‑24 (MQ=255)

                                                        cGGCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtactttt                    <  1:2307217‑M1/76‑57 (MQ=255)

                                                          gCTGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgc                  <  2:837517‑M1/76‑59 (MQ=255)

                                                            tGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggctgatgtagcactggtaggctttggtacttttgccc                >  2:4324239‑M1/1‑16 (MQ=255)

                                                            tGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccg                <  1:9255972‑M1/76‑61 (MQ=255)

                                                            tGGCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccg                >  1:2699257‑M1/1‑16 (MQ=255)

                                                             ggCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgt               >  1:184370‑M1/1‑15 (MQ=255)

                                                              gCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgtt              <  2:1570263‑M1/76‑63 (MQ=255)

                                                              gCCGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgtt              >  2:6983145‑M1/1‑14 (MQ=255)

                                                                cGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaa            <  1:3180481‑M1/76‑65 (MQ=255)

                                                                cGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaa            >  2:6457570‑M1/1‑12 (MQ=255)

                                                                cGTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaa            >  2:3262294‑M1/1‑12 (MQ=255)

                                                                 gTGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaa           >  1:9198262‑M1/1‑11 (MQ=255)

                                                                  tGCGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaag          <  1:8632212‑M1/76‑67 (MQ=255)

                                                                    cGTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagag        <  2:95791‑M1/76‑69 (MQ=255)

                                                                     gTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagc       >  1:8245184‑M1/1‑7 (MQ=255)

                                                                     gTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagc       <  1:7927494‑M1/76‑70 (MQ=255)

                                                                     gTTAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagc       >  2:3729019‑M1/1‑7 (MQ=255)

                                                                      ttAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcg      >  1:9653584‑M1/1‑6 (MQ=255)

                                                                      ttAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcg      <  1:10301440‑M1/76‑71 (MQ=255)

                                                                       tAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcgt     <  2:577954‑M1/76‑72 (MQ=255)

                                                                       tAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcgt     <  1:5275706‑M1/76‑72 (MQ=255)

                                                                       tAGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcgt     >  2:1977446‑M1/1‑5 (MQ=255)

                                                                        aGATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcgtg    >  2:10090524‑M1/1‑4 (MQ=255)

                                                                         gATtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcgtgc   <  1:7069624‑M1/76‑74 (MQ=255)

                                                                          aTtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcgtgct  >  2:9182500‑M1/1‑2 (MQ=255)

                                                                          aTtaactgaatctctgaaagaaggggatgatgtagcactggtaggttttggtacttttgccgttaaagagcgtgct  >  2:2521230‑M1/1‑2 (MQ=255)

GAATAAATCTCAATTGATCGACAAGATTGCTGCAGGGGCTGATATCTCTAAAGCTGCGGCTGGCCGTGCGTTAGATGCTATTATTGCTTCCGTAACTGAATCTCTGAAAGAAGGGGATGATGTAGCACTGGTAGGTTTTGGTACTTTTGC  >  REL606/433508‑433657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`