Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	1,812,834	IS150 (+) +3 bp	coding (92‑94/549 nt)	ynjA →	hypothetical protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	664688 =	NA (NA)	83 (1.330)	51/150	0.1	100%	noncoding (1/1443 nt)	IS150	repeat region
	?	REL606	= 1812836	0 (0.000)					coding (94/549 nt)	ynjA	hypothetical protein
*	?	REL606	1812834 =	0 (0.000)	58 (0.960)	46/146	0.2	100%	coding (92/549 nt)	ynjA	hypothetical protein
	?	REL606	2774435 =	NA (NA)					noncoding (1443/1443 nt)	IS150	repeat region

AGCGTTTGCGCTCCAGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAAtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/1812908‑1812834 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACC  >  REL606/2774435‑2774511                                                                                                                                                          AGCGTTTGCGCTCCAGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAAT                                                                              >  1:1295778/1‑76   CGTTTGCGCTCCAGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGT                                                                            <  2:1914573/76‑1   CGTGTGCGCTCCGGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGT                                                                            <  1:706759/76‑1      TTGCGCTACAGCCAGCAAACAAACATCGACTCCAGATAACAGATAAACGGGATCCGCCCCCACCAGCGAATGTACT                                                                         <  1:35911/76‑1        GCGCTCCAGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGA                                                                       <  2:827447/76‑1            TCCAGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCC                                                                   >  2:1453440/1‑76               AGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAA                                                                >  2:1338596/1‑76                GCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAA                                                               <  1:1533437/76‑1                        ACAAACATCGACACCAGATAAAAGACAACCGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGAC                                                       <  1:315630/76‑1                        ACAAACANCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGAC                                                       >  2:1649912/1‑76                          AAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAG                                                     <  2:1965245/76‑1                            ACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTT                                                   <  1:416617/76‑1                             CATCGACACCAGATAAAATATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTA                                                  <  2:972482/76‑1                              ATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAA                                                 >  1:909891/1‑76                               TCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAA                                                >  1:457752/1‑76                               TCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAA                                                >  1:812510/1‑76                               TCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAA                                                >  1:553463/1‑76                                CGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGCCAAAC                                               <  1:1916620/76‑1                                 GACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACA                                              <  1:439114/76‑1                                  ACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACAC                                             >  1:784354/1‑76                                       AGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGC                                        >  1:1189602/1‑76                                         aaAAAAGAAAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                      <  1:904490/74‑1                                         aaAAAAAATAAAGGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCAT                                      <  1:887302/74‑1                                          aAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATA                                     <  1:473189/75‑1                                          TAAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATA                                     >  2:1198592/1‑76                                           AAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTGCTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATAT                                    <  2:1357480/76‑1                                           AAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATAT                                    <  2:642817/76‑1                                           AAAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATAT                                    <  1:927170/76‑1                                            AAAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATA                                   >  1:706466/1‑76                                             AAGATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAG                                  <  1:124982/76‑1                                                ATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                               >  1:1248857/1‑76                                                ATAAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTC                               <  2:1345062/76‑1                                                  AAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAATTTGGACAGTTAAACACGAGGCATATAGGTCTG                             <  2:1412956/76‑1                                                  AAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                             >  2:1079698/1‑76                                                  AAACGGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTG                             <  2:1433959/76‑1                                                      GGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                         >  1:547321/1‑76                                                      GGGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTC                         >  2:1336283/1‑76                                                       GGATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCC                        <  1:1816403/76‑1                                                        GATCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCG                       >  2:1198381/1‑76                                                          TCCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGAT                     <  1:812498/76‑1                                                           CCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATA                    <  1:1616136/76‑1                                                           CCGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATA                    >  1:962106/1‑76                                                            CGCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATAT                   <  2:513399/76‑1                                                             GCCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATT                  >  2:738120/1‑76                                                              CCCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTC                 <  2:1046386/76‑1                                                               CCCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCA                >  2:631184/1‑76                                                                CCCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAA               >  2:1148321/1‑76                                                                 CCACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAAT              <  1:1169305/76‑1                                                                  CACCAGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATT             <  1:1935558/76‑1                                                                      AGCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAG         >  2:851116/1‑76                                                                       GCGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGT        <  2:369067/76‑1                                                                        CGAATGTACTGACCCCAAGAAGATGGANAGNTANACTCGAGNCACATACGTCTGATTCCGATATTCAATTGGAGTC       <  1:1760410/76‑1                                                                        CGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC       >  1:148066/1‑76                                                                        CGAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTC       <  2:1289486/76‑1                                                                         GAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA      <  1:381089/76‑1                                                                         GAATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCA      >  1:885446/1‑76                                                                          AATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG     >  2:754070/1‑76                                                                          AATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAG     <  1:1336284/76‑1                                                                           ATGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGA    <  2:271761/76‑1                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   >  2:229123/1‑76                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   <  1:542090/76‑1                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   >  1:714913/1‑76                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   >  2:248633/1‑76                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   >  1:1097104/1‑76                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   <  2:1361974/76‑1                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   >  1:1477242/1‑76                                                                            TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   <  2:1065727/76‑1                                                                            TGTACTGACCCCAAAAAGTGGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGAC   <  2:549624/76‑1                                                                             GTACTGACCCCCAAAAGTTGGACAGTTAAGCACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACC  >  1:63546/1‑76                                                                             GTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACC  >  1:873899/1‑76                                                                             GTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACC  >  1:1274045/1‑76                                                                             GTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACC  >  1:1423160/1‑76                                                                             GTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACC  >  1:1514825/1‑76                                                                             GTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGCTCTGATTCCGATATTCCATTGGAGTCAGACC  >  2:1799097/1‑76                                                                                                                                                          AGCGTTTGCGCTCCAGCCAGCCAACAAACATCGACACCAGATAAAAGATAAACGGGATCCGCCCCCACCAGCGAAtg‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  REL606/1812908‑1812834 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGTACTGACCCCAAAAAGTTGGACAGTTAAACACGAGGCATATAGGTCTGATTCCGATATTCAATTGGAGTCAGACC  >  REL606/2774435‑2774511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.