BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	REL606	3,894,997	Δ6,934 bp	IS150‑mediated	rbsD–[yieO]	rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	REL606	3893608–3894994	3901930	6937–8323	123 [112]	[0] 313	[insJ‑5]–[yieO]	[insJ‑5],insK‑5,rbsD,rbsA,rbsC,rbsB,rbsK,rbsR,[yieO]

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 3652533	NA (NA)	299 (1.140)	113/146	0.1	100%	noncoding (1443/1443 nt)	IS150	repeat region
*	?	REL606	3901931 =	0 (0.000)	299 (1.140)	113/146	0.1	100%	coding (489/1428 nt)	yieO	predicted multidrug or homocysteine efflux system

Only 100 of 123 total aligned reads displayed.

AGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893532‑3893627

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  2:7127966/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  1:733592/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  1:4760441/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  2:6044550/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  2:6317555/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  1:3403360/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  2:7447277/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  1:2061573/76‑1 (MQ=255)

aGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaa                      <  2:7523372/76‑1 (MQ=255)

 ggTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCaaa                     <  1:5162139/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:3316686/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  2:8822574/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  2:1191472/76‑1 (MQ=255)

  gTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAg                    <  1:7561578/76‑1 (MQ=255)

   tCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGc                   <  2:7193121/76‑1 (MQ=255)

   tCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGc                   <  2:653556/76‑1 (MQ=255)

   tCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGc                   <  1:1280502/76‑1 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  >  1:3298018/1‑76 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  >  2:3978615/1‑76 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  >  1:4438236/1‑76 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  <  2:4487727/76‑1 (MQ=255)

    cGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGcc                  <  2:4247506/76‑1 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 >  2:1498163/1‑76 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  2:8141977/76‑1 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 <  1:7525020/76‑1 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 >  2:4477664/1‑76 (MQ=255)

     gAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCa                 >  1:250422/1‑76 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                <  1:130254/76‑1 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  2:1553707/1‑76 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                <  1:2643935/76‑1 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  1:8941049/1‑76 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  1:2296974/1‑76 (MQ=255)

      aaaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaa                >  2:8221382/1‑76 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  2:1328250/76‑1 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  2:8465339/76‑1 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  2:1797244/76‑1 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               >  2:928761/1‑76 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  1:4008604/76‑1 (MQ=255)

       aaTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaa               <  1:3180187/76‑1 (MQ=255)

        aTCTGACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaaa              <  1:4274556/76‑1 (MQ=255)

        aTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCaaaa              <  2:5074840/76‑1 (MQ=255)

        aTCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGCAATAGCCCCTAATATGTCAAAGCCaaaa              <  2:2315140/76‑1 (MQ=255)

         tCTAACGCCAGCCTGTACTGCTCCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  2:1934515/76‑1 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  2:7415529/76‑1 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  1:6141218/76‑1 (MQ=255)

         tCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAAt             <  1:8871229/76‑1 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            <  2:2456057/76‑1 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            >  1:3962562/1‑76 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            >  2:720488/1‑76 (MQ=255)

          cTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATa            <  1:1953072/76‑1 (MQ=255)

           tAACGCCAGACTGTAGTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           <  1:2232928/76‑1 (MQ=255)

           tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           >  1:6119502/1‑76 (MQ=255)

           tAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAc           >  1:1121715/1‑76 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGGTGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  2:3884530/1‑76 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          <  1:7685142/76‑1 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  1:6076065/1‑76 (MQ=255)

            aaCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAcc          >  2:480066/1‑76 (MQ=255)

             ccGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAccc         <  1:7480240/75‑1 (MQ=255)

             aCGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATAccc         <  2:8053590/76‑1 (MQ=255)

              cGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCt        <  1:8711122/76‑1 (MQ=40)

              cGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCt        <  1:4522809/76‑1 (MQ=40)

               gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       <  1:6579206/76‑1 (MQ=39)

               gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       <  1:8203348/76‑1 (MQ=39)

               gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       <  2:6849798/76‑1 (MQ=39)

               gCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtt       <  2:5086762/76‑1 (MQ=39)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      <  2:7086789/76‑1 (MQ=38)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      <  1:7239470/76‑1 (MQ=38)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      >  1:1489611/1‑76 (MQ=38)

                ccAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCttt      <  1:799875/76‑1 (MQ=38)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     >  1:5351860/1‑76 (MQ=35)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     <  2:3595890/76‑1 (MQ=35)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     <  2:2012502/76‑1 (MQ=35)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     <  1:8866878/76‑1 (MQ=35)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     <  1:4163632/76‑1 (MQ=35)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     <  1:1073110/76‑1 (MQ=35)

                 cAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCtttt     <  1:4560650/76‑1 (MQ=35)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  2:1168987/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  1:4619156/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  1:4871893/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  1:5481914/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  2:8385427/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  2:7754436/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    <  2:8071504/76‑1 (MQ=34)

                  aGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTg    >  2:2934415/1‑76 (MQ=34)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCTCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:8673549/1‑76 (MQ=17)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:2696593/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:8673550/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:1785125/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:8426553/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  1:1969959/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:7928465/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:3606535/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:4282597/1‑76 (MQ=32)

                   gACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGa   >  2:2598743/1‑76 (MQ=32)

                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  >  2:7536472/1‑76 (MQ=31)

                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:6770891/76‑1 (MQ=31)

                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  <  1:2002239/76‑1 (MQ=31)

                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  >  2:648243/1‑76 (MQ=31)

                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  >  2:2730965/1‑76 (MQ=31)

                    aCTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGaa  >  2:5096977/1‑76 (MQ=31)

AGGTCGAAATCTAACGCCAGACTGTACTGCACCCATTTTGTTGGACGATGAAATGGAATAGCCCCTAATATGTCAAAGCCAAAATACCCTTTTGAA  >  REL606/3893532‑3893627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`