Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	REL606	474,423	IS1 (+) +8 bp	coding (876‑883/1677 nt)	ybaL ←	predicted transporter with NAD(P)‑binding Rossmann‑fold domain

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	474423 =	4 (0.020)	359 (1.490)	114/150	0.1	98.8%	coding (883/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	589555 =	NA (NA)					noncoding (768/768 nt)	IS1	repeat region
*	?	REL606	= 474430	4 (0.020)	327 (1.360)	113/150	0.1	98.7%	coding (876/1677 nt)	ybaL	predicted transporter with NAD(P)‑binding Rossmann‑fold domain
	?	REL606	= 547699	NA (NA)					noncoding (1/768 nt)	IS1	repeat region

TGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTACCCTGATACTGAGCAGCAAAGT  >  REL606/547626‑547770                                                                          |                                                                        tGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATcaccac                                                                       >  1:1430615/1‑76 (MQ=31) tGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATcaccac                                                                       >  1:1893343/1‑76 (MQ=31)         aCAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACCGAAACCa                                                               >  2:7734717/1‑76 (MQ=40)             aaGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACCGAAACCATCAc                                                           >  1:3551495/1‑76 (MQ=255)                  aCTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTg                                                      >  1:5185050/1‑76 (MQ=255)                     ggAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCt                                                   >  1:1793698/1‑76 (MQ=255)                                            tACACTAAATCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCAtt                            >  1:7529056/1‑76 (MQ=255)                                                 tAAATCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTAc                       >  1:4643775/1‑76 (MQ=255)                                                  aaaTCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTAcc                      >  1:3181687/1‑76 (MQ=255)                                                  aaaTCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTAcc                      >  1:7843180/1‑76 (MQ=255)                                                   aaTCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTAccc                     >  1:1454109/1‑76 (MQ=255)                                                         tAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTACCCTGATAc               >  2:2104162/1‑76 (MQ=255)                                                                      tCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTACCCTGATACTGAGCAGCAAAGt  >  1:4373229/1‑76 (MQ=255)                                                                          |                                                                        TGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCACCGAAACCATCACCATTGGCTTCAGTTACGTCAGTGCGGTCATTTTTACCCTGATACTGAGCAGCAAAGT  >  REL606/547626‑547770

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.