| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | REL606 | 3,014,099 | (ACAGGC)3→4 | coding (680/1137 nt) | ECB_02815 → | Capsule polysaccharide export inner‑membrane protein kpsE |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 3014081 = | 20 (0.090) | 215 (1.210) | 96/124 | 0.0 | 96.3% | coding (662/1137 nt) | ECB_02815 | Capsule polysaccharide export inner‑membrane protein kpsE |
| ? | REL606 | = 3014086 | 0 (0.000) | coding (667/1137 nt) | ECB_02815 | Capsule polysaccharide export inner‑membrane protein kpsE | |||||
Only 100 of 560 total aligned reads displayed.
GCACGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAGCGGACCTGCGGA > REL606/3014011‑3014161 | cagcgtctggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacagg < 1:99775‑M1/6‑1 (MQ=255) aCGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGca < 2:5867978‑M2/76‑3 (MQ=255) cgtctggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcac > 1:5218519‑M1/68‑76 (MQ=255) cgtctggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcac < 2:1054911‑M1/9‑1 (MQ=255) gtctggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcaca > 2:6712956‑M1/67‑76 (MQ=255) tctggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacag > 2:396503‑M1/66‑76 (MQ=255) ctggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacagg > 1:5359024‑M1/65‑76 (MQ=255) ctggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacagg < 2:698682‑M1/12‑1 (MQ=255) cAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacagg > 1:6163106‑M2/1‑70 (MQ=255) tcgacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttcgggatccacaggcacaggcacaggc > 2:4827574‑M1/64‑76 (MQ=255) aGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggc < 2:5455646‑M2/76‑8 (MQ=255) aGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggc < 2:235314‑M2/76‑8 (MQ=255) ggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacaggca > 2:5363425‑M1/63‑76 (MQ=255) ggacgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacaggca > 1:5021304‑M1/63‑76 (MQ=255) gCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggca < 1:6896375‑M2/76‑9 (MQ=255) gTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcaca < 1:2723879‑M2/76‑11 (MQ=255) gTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcaca < 1:2184577‑M2/76‑11 (MQ=255) gTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcaca > 2:4904781‑M2/1‑66 (MQ=255) acgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacaggcaca > 1:5453390‑M1/61‑76 (MQ=255) cgccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacaggcacag > 1:4429636‑M1/60‑76 (MQ=255) gccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacaggcacagg > 1:3368879‑M1/59‑76 (MQ=255) gccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacaggcacagg < 2:3330469‑M1/18‑1 (MQ=255) gccagcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggcacaggcacaggcacagg > 2:6058578‑M1/59‑76 (MQ=255) gACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcac > 2:2624309‑M2/1‑61 (MQ=255) agcaaagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAggcgg > 1:491467‑M1/56‑76 (MQ=255) aCGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcaca > 1:5065229‑M2/1‑60 (MQ=255) cGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacag > 1:6278062‑M2/1‑59 (MQ=255) cGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacag > 2:1630743‑M2/1‑59 (MQ=255) gCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacagg > 1:6687628‑M2/1‑58 (MQ=255) ccAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggc < 1:547793‑M2/76‑20 (MQ=255) aagcggaattgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAg > 2:2238335‑M1/52‑76 (MQ=255) aGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcgg > 2:3687109‑M2/1‑55 (MQ=255) aaaGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcga < 1:4355940‑M2/76‑25 (MQ=255) aaaGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGAATCCACAGGcacaggcacaggcacacgcggcca < 2:5543491‑M2/76‑25 (MQ=255) aattgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGtt < 1:4543206‑M1/31‑1 (MQ=255) aattgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGtt > 1:1607124‑M1/46‑76 (MQ=255) aattgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGtt > 2:1213092‑M1/46‑76 (MQ=255) aaGCTGAATTGCTCTCTTATCAAGACAACAACAGCGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgag > 1:3757950‑M2/1‑51 (MQ=255) attgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTa > 1:2330230‑M1/45‑76 (MQ=255) ttgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAg > 2:257911‑M1/44‑76 (MQ=255) tgctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCacgtttgt < 1:2840816‑M1/34‑4 (MQ=255) gAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgt < 1:732857‑M2/76‑31 (MQ=255) ctctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGa < 1:2466445‑M1/36‑1 (MQ=255) tctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGaa < 1:5312216‑M1/37‑1 (MQ=255) tctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGaa > 1:6550503‑M1/40‑76 (MQ=255) tctcttatcaagacaacaacaacgttctggatccacaggCACAGGAACAGGCACAGGCGGCGAGCACGATAGTGaa < 1:1009294‑M1/37‑1 (MQ=255) ctcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAAt > 1:5839039‑M1/39‑76 (MQ=255) aTTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgtta > 2:2756145‑M2/1‑44 (MQ=255) ttGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttag > 1:2552761‑M2/1‑43 (MQ=255) tcttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATa > 1:2057575‑M1/38‑76 (MQ=255) cttatcaagacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATAc > 2:84693‑M1/37‑76 (MQ=255) ctctctTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtga < 1:3451089‑M2/76‑37 (MQ=255) tctctTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaa < 1:413830‑M2/76‑38 (MQ=255) ctctTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaat > 1:32286‑M2/1‑38 (MQ=255) ctctTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaat < 2:6747690‑M2/76‑39 (MQ=255) ctctTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaat > 2:215798‑M2/1‑38 (MQ=255) ctTATCAAGACAACAACAACGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatac > 1:6612648‑M2/1‑36 (MQ=255) agacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATg < 2:2567563‑M1/47‑1 (MQ=255) agacaacaacaacgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATg > 2:1889390‑M1/30‑76 (MQ=255) caacaacaacgttctggatccacaggCACAGGCACAGGTACAGGCGGCGAGCACGTTAGTGAATACGCTGGtgggg < 2:1831544‑M1/50‑2 (MQ=255) cAAGACAACAACAACGTTCTGGATCCACAGGcacaggcagaggcacaggcggcgagcacgttagtgaaaacgctga < 2:4334678‑M2/76‑46 (MQ=255) caacaacaaCGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggg < 2:3542355‑M2/76‑51 (MQ=255) aacaacaacGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggcc > 2:3187638‑M2/1‑25 (MQ=255) aacaacaacGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgcctatgggcc < 1:6331062‑M2/76‑52 (MQ=255) acgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGGCAGAAGAt < 2:4711198‑M1/58‑1 (MQ=255) acgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGAt > 2:6601632‑M1/19‑76 (MQ=255) acgttctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGAt > 1:6121282‑M1/19‑76 (MQ=255) acaacaacGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggcca < 1:3998554‑M2/76‑53 (MQ=255) aacaacGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccaga < 1:2958987‑M2/76‑55 (MQ=255) caacGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaag > 1:3836934‑M2/1‑20 (MQ=255) ctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGa < 1:6038654‑M1/63‑1 (MQ=255) ctggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAACAACCAGa < 1:2537530‑M1/63‑1 (MQ=255) aacGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaaga < 2:2934730‑M2/76‑58 (MQ=255) tggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGGTGGGCCAGAAGATCCAGAt < 1:6499170‑M1/64‑1 (MQ=255) tggagccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGAt > 1:3627512‑M1/13‑76 (MQ=255) ggatccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATg > 1:1964220‑M1/12‑76 (MQ=255) cGTTCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatc < 1:2144418‑M2/76‑60 (MQ=255) ttCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatcca < 2:2487332‑M2/76‑62 (MQ=255) ttCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatcca > 2:4567669‑M2/1‑15 (MQ=255) ttCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatcca < 2:6867912‑M2/76‑62 (MQ=255) ttCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagataca < 1:1845465‑M2/76‑62 (MQ=255) atccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGa > 1:1698630‑M1/10‑76 (MQ=255) tCTGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccag > 2:4067530‑M2/1‑14 (MQ=255) ccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGGAGATCCCgatgggag < 2:3123955‑M1/69‑4 (MQ=255) ccacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAg > 1:1352444‑M1/8‑76 (MQ=255) tGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagat > 1:3561800‑M2/1‑12 (MQ=255) tGGATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagat > 1:469107‑M2/1‑12 (MQ=255) cacaggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATAAGCTGATGGGCCAGAAGATCCAGATGGAAGc < 2:5751593‑M1/70‑1 (MQ=255) ggATCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatg > 1:1093796‑M2/1‑11 (MQ=255) aTCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgattggccagaagatcccgatgga < 2:1418111‑M2/76‑68 (MQ=255) aTCCACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatgga < 2:6215622‑M2/76‑68 (MQ=255) ggCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAGCGGAc > 2:3781843‑M1/3‑76 (MQ=255) ccACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaag > 2:4417213‑M2/1‑7 (MQ=255) ccACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaag > 2:2018899‑M2/1‑7 (MQ=255) ccACAGGcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaag > 1:5628868‑M2/1‑7 (MQ=255) CACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAGCGGACCt > 2:4157325‑M1/1‑76 (MQ=255) CACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAGCGGACCt > 1:4204431‑M1/1‑76 (MQ=255) caggcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcgg < 2:5122649‑M2/76‑73 (MQ=255) caggcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcgg > 2:1273002‑M2/1‑4 (MQ=255) ggcacaggcacaggcacaggcggcgagcacgttagtgaatacgctgatgggccagaagatccagatggaagcggac > 1:6811837‑M2/1‑2 (MQ=255) | GCACGCCAGCGTCTGGACGCCAGCAAAGCGGAATTGCTCTCTTATCAAGACAACAACAACGTTCTGGATCCACAGGCACAGGCACAGGCGGCGAGCACGTTAGTGAATACGCTGATGGGCCAGAAGATCCAGATGGAAGCGGACCTGCGGA > REL606/3014011‑3014161 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |