Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	REL606	4,163,890	Δ9 bp	coding (3035‑3043/4029 nt)	rpoB →	DNA‑directed RNA polymerase subunit beta

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	REL606	= 4163889	1 (0.020)	57 (0.970)	45/136	0.2	98.4%	coding (3034/4029 nt)	rpoB	DNA‑directed RNA polymerase subunit beta
	?	REL606	4163899 =	1 (0.020)					coding (3044/4029 nt)	rpoB	DNA‑directed RNA polymerase subunit beta

GAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4163808‑4163889 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagctggCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCG  >  REL606/4163899‑4163973                                                                                                                                                               GAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATC                                                                                   <  1:106683/76‑1      TGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTG                                                                              >  2:117121/1‑76      TGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTG                                                                              >  1:730363/1‑76       GAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGG                                                                             <  1:1114924/76‑1         GAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCT                                                                           <  2:877604/76‑1         GAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCT                                                                           <  2:678342/76‑1         GAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCT                                                                           >  2:257920/1‑76          AAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTG                                                                          <  2:542241/76‑1             CTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGC                                                                       <  2:1866441/76‑1                 ACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTA                                                                   >  2:708697/1‑76                 ACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTA                                                                   >  2:1797913/1‑76                  CAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTAT                                                                  >  1:1247271/1‑76                  CAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTAT                                                                  >  2:1270860/1‑76                   AAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATG                                                                 <  2:2053177/76‑1                     ACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGAC                                                               <  1:264622/76‑1                           GCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTG                                                         <  1:418224/76‑1                           GCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTG                                                         >  2:1552859/1‑76                             GCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAA                                                       >  2:1312063/1‑76                              CGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAA                                                      >  1:1202184/1‑76                              CGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAA                                                      <  1:669237/76‑1                                  CGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACG                                                  <  1:942641/76‑1                                   GCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGA                                                 >  1:1132176/1‑76                                    CTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAG                                                <  2:1807536/76‑1                                    CTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAG                                                >  2:623544/1‑76                                      GGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTT                                              >  1:98867/1‑76                                        CTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCG                                            <  2:450257/76‑1                                        CTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCG                                            <  2:1544188/76‑1                                        CTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCG                                            >  1:1530732/1‑76                                         TGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGA                                           >  1:1534396/1‑76                                         TGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGA                                           >  1:198418/1‑76                                          GGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAG                                          >  1:744699/1‑76                                           GAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGA                                         <  2:885222/76‑1                                               TGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAA                                     >  1:1233105/1‑76                                               TGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAA                                     >  2:1894167/1‑76                                                GGGCCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAA                                    <  1:780953/76‑1                                                   CCTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTC                                 >  1:1857983/1‑76                                                    CTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCG                                <  2:802476/76‑1                                                    CTGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCG                                >  1:2047062/1‑76                                                     TGACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGA                               >  2:383868/1‑76                                                       ACCGACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAG                             >  2:1239056/1‑76                                                          GACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGA                          <  1:883758/76‑1                                                          GACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGA                          >  2:572251/1‑76                                                           ACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAn                         <  1:563113/76‑2                                                           ACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAA                         >  1:1250999/1‑76                                                           ACGAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAA                         <  2:1692537/76‑1                                                             GAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAAC                       <  1:836072/76‑1                                                             GAAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAAC                       >  1:147732/1‑76                                                              AAGAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACG                      >  2:1967597/1‑76                                                                GAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCC                    <  2:578069/76‑1                                                                GAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCC                    <  2:415802/76‑1                                                                GAGAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACACGAAGCGAAGCGCC                    <  2:1526683/76‑1                                                                  GAAACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGC                  >  1:872556/1‑76                                                                    AACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAA                >  2:283612/1‑76                                                                     ACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAA               >  1:810703/1‑76                                                                     ACAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAA               >  1:461657/1‑76                                                                      CAAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAA              <  2:1249276/76‑1                                                                       AAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAAT             <  2:1351121/76‑1                                                                       AAAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAAT             <  2:211855/76‑1                                                                        AAATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATC            >  2:1579710/1‑76                                                                          ATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCAC          >  1:456341/1‑76                                                                          ATCAGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCAC          >  2:1194608/1‑76                                                                             AGCTGGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCA       <  1:2030199/76‑1                                                                                 GGCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGC   >  1:568192/1‑76                                                                                  GCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCG  >  2:1432480/1‑76                                                                                  GCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCG  >  1:1308388/1‑76                                                                                                                                                               GAAGCTGAGAAGCTCGACAAACTGCCGCGCGATCGCTGGCTGGAGCTGGGCCTGACCGACGAAGAGAAACAAAATCAGCTGG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  REL606/4163808‑4163889 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cagctggCTGAGCAGTATGACGAACTGAAACACGAGTTCGAGAAGAAACTCGAAGCGAAACGCCGCAAAATCACCCAGGGCG  >  REL606/4163899‑4163973

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.