mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | R2_Lane7_BC12_R1.good.fq | 1,537,223 | 116,757,865 | 100.0% | 76.0 bases | 76 bases | 97.7% |
| errors | R2_Lane7_BC12_R2.good.fq | 1,537,223 | 116,757,865 | 100.0% | 76.0 bases | 76 bases | 94.4% |
| total | 3,074,446 | 233,515,730 | 100.0% | 76.0 bases | 76 bases | 96.0% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | REL606 | 4,629,812 | 47.9 | 2.7 | 100.0% | Escherichia coli strain REL606. |
| total | 4,629,812 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 7739 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 223 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.008 |
| reference sequence | pr(no read start) |
|---|---|
| REL606 | 0.74975 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 02:01:07 02 Feb 2019 | 02:01:39 02 Feb 2019 | 32 seconds |
| Read alignment to reference genome | 02:01:40 02 Feb 2019 | 02:06:05 02 Feb 2019 | 4 minutes 25 seconds |
| Preprocessing alignments for candidate junction identification | 02:06:05 02 Feb 2019 | 02:06:42 02 Feb 2019 | 37 seconds |
| Preliminary analysis of coverage distribution | 02:06:42 02 Feb 2019 | 02:07:58 02 Feb 2019 | 1 minute 16 seconds |
| Identifying junction candidates | 02:07:58 02 Feb 2019 | 02:08:00 02 Feb 2019 | 2 seconds |
| Re-alignment to junction candidates | 02:08:00 02 Feb 2019 | 02:09:09 02 Feb 2019 | 1 minute 9 seconds |
| Resolving best read alignments | 02:09:09 02 Feb 2019 | 02:10:04 02 Feb 2019 | 55 seconds |
| Creating BAM files | 02:10:04 02 Feb 2019 | 02:11:15 02 Feb 2019 | 1 minute 11 seconds |
| Tabulating error counts | 02:11:15 02 Feb 2019 | 02:11:35 02 Feb 2019 | 20 seconds |
| Re-calibrating base error rates | 02:11:35 02 Feb 2019 | 02:11:36 02 Feb 2019 | 1 second |
| Examining read alignment evidence | 02:11:36 02 Feb 2019 | 02:14:52 02 Feb 2019 | 3 minutes 16 seconds |
| Polymorphism statistics | 02:14:52 02 Feb 2019 | 02:14:52 02 Feb 2019 | 0 seconds |
| Output | 02:14:52 02 Feb 2019 | 02:15:03 02 Feb 2019 | 11 seconds |
| Total | 13 minutes 55 seconds | ||
